Restriction map for
pF1KE0702
(Circular) (Six-base) MAPSORT of: pF1KE0702.seq Check: 7347 from: 1 to: 4331
pF1KE0702 4331 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 915 915
Size: 4331
AatII G_ACGT'C
Cuts at: 194 194
Size: 4331
AccI GT'mk_AC
Cuts at: 749 1369 749
Size: 620 3711
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1289 2156 2588 3335 3603 589
Size: 700 867 432 747 268 1317
Fragments arranged by size:
1317 867 747 700 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 825 1636 3203 825
Size: 811 1567 1953
Fragments arranged by size:
1953 1567 811
ApaI G_GGCC'C
Cuts at: 1267 1267
Size: 4331
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 574 871 1351 574
Size: 297 480 3554
Fragments arranged by size:
3554 480 297
BamHI G'GATC_C
Cuts at: 930 1356 930
Size: 426 3905
BanI G'GyrC_C
Cuts at: 809 1148 1347 1761 2009 2044 809
Size: 339 199 414 248 35 3096
Fragments arranged by size:
3096 414 339 248 199 35
BanII G_rGCy'C
Cuts at: 214 1267 1345 2375 214
Size: 1053 78 1030 2170
Fragments arranged by size:
2170 1053 1030 78
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 395 395
Size: 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 729 763 1147 1181 729
Size: 34 384 34 3879
Fragments arranged by size:
3879 384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 839 1851 839
Size: 1012 3319
BglI GCCn_nnn'nGGC
Cuts at: 1245 1245
Size: 4331
BglII A'GATC_T
Cuts at: 564 1846 3883 564
Size: 1282 2037 1012
Fragments arranged by size:
2037 1282 1012
BlpI GC'TnA_GC
Cuts at: 284 953 1443 284
Size: 669 490 3172
Fragments arranged by size:
3172 669 490
Bme1580I G_kGCm'C
Cuts at: 1187 1267 1956 2049 3105 1187
Size: 80 689 93 1056 2413
Fragments arranged by size:
2413 1056 689 93 80
BmgBI CAC'GTC
Cuts at: 120 120
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 156 1197 1543 1624 1959 4103 156
Size: 1041 346 81 335 2144 384
Fragments arranged by size:
2144 1041 384 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1299 2698 1299
Size: 1399 2932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 897 328
Size: 569 3762
Bpu10I CC'TnA_GC
Cuts at: 299 1075 299
Size: 776 3555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 518 4035 518
Size: 3517 814
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 191 1762 2010 2712 4208 191
Size: 1571 248 702 1496 314
Fragments arranged by size:
1571 1496 702 314 248
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 895 925 838
Size: 30 27 30 4244
Fragments arranged by size:
4244 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 325 1288 218
Size: 107 963 3261
Fragments arranged by size:
3261 963 107
BseYI C'CCAG_C
Cuts at: 1320 1756 2514 3091 3587 1320
Size: 436 758 577 496 2064
Fragments arranged by size:
2064 758 577 496 436
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 214 1345 2123 2313 3105 214
Size: 1131 778 190 792 1440
Fragments arranged by size:
1440 1131 792 778 190
BsmI GAATG_Cn'
Cuts at: 1113 1113
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 214 1187 1267 1345 1956 2049 2123 2313 2375
Size: 973 80 78 611 93 74 190 62
Cuts at: 2375 3105 214
Size: 730 1440
Fragments arranged by size:
1440 973 730 611 190 93 80 78
74 62
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 915 1381 1595 1897 2278 915
Size: 466 214 302 381 2968
Fragments arranged by size:
2968 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 288 2623 2677 4161 4326 50
Size: 238 2335 54 1484 165 55
Fragments arranged by size:
2335 1484 238 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 361 812 1235 2329 2510 2719 361
Size: 451 423 1094 181 209 1973
Fragments arranged by size:
1973 1094 451 423 209 181
BssSI C'ACGA_G
Cuts at: 250 2602 2960 250
Size: 2352 358 1621
Fragments arranged by size:
2352 1621 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 114 806 114
Size: 692 3639
BstYI r'GATC_y
Cuts at: 1 564 605 698 821 930 1356 1823 1846
Size: 563 41 93 123 109 426 467 23
Cuts at: 1846 2181 2427 3439 3525 3537 3883 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 563 467 449 426 346 335 246
123 109 93 86 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 185 935 185
Size: 750 3581
BtgI C'CryG_G
Cuts at: 713 713
Size: 4331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 533 1046 1574 533
Size: 513 528 3290
Fragments arranged by size:
3290 528 513
ClaI AT'CG_AT
Cuts at: 389 389
Size: 4331
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 246 363 1093 1916 2090 2481 2508 3853 246
Size: 117 730 823 174 391 27 1345 724
Fragments arranged by size:
1345 823 730 724 391 174 117 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 1265 2354 2564 1265
Size: 1089 210 3032
Fragments arranged by size:
3032 1089 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 2539 2849 2995 4141 211
Size: 2328 310 146 1146 401
Fragments arranged by size:
2328 1146 401 310 146
EcoICRI GAG'CTC
Cuts at: 212 1343 212
Size: 1131 3200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 589 897 1289 2156 2588 3335 3603 328
Size: 261 308 392 867 432 747 268 1056
Fragments arranged by size:
1056 867 747 432 392 308 268 261
EcoNI CCTnn'n_nnAGG
Cuts at: 352 352
Size: 4331
EcoO109I rG'GnC_Cy
Cuts at: 674 960 1122 1264 1470 674
Size: 286 162 142 206 3535
Fragments arranged by size:
3535 286 206 162 142
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 694 1765 2013 3035 4014 694
Size: 1071 248 1022 979 1011
Fragments arranged by size:
1071 1022 1011 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 895 927 2173 2203 2205 2235 895
Size: 32 1246 30 2 30 2991
Fragments arranged by size:
2991 1246 32 30 30 2
HincII GTy'rAC
Cuts at: 750 1370 750
Size: 620 3711
HindIII A'AGCT_T
Cuts at: 655 655
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 633 3002 3186 633
Size: 2369 184 1778
Fragments arranged by size:
2369 1778 184
MscI TGG'CCA
Cuts at: 248 2092 248
Size: 1844 2487
MslI CAynn'nnrTG
Cuts at: 841 2320 2447 841
Size: 1479 127 2725
Fragments arranged by size:
2725 1479 127
MspA1I CmG'CkG
Cuts at: 283 306 952 1026 1442 1756 2116 3129 3374
Size: 23 646 74 416 314 360 1013 245
Cuts at: 3374 283
Size: 1240
Fragments arranged by size:
1240 1013 646 416 360 314 245 74
23
NaeI GCC'GGC
Cuts at: 363 2512 363
Size: 2149 2182
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 713 713
Size: 4331
NgoMIV G'CCGG_C
Cuts at: 361 2510 361
Size: 2149 2182
NspI r_CATG'y
Cuts at: 1384 1884 2791 1384
Size: 500 907 2924
Fragments arranged by size:
2924 907 500
PasI CC'CwG_GG
Cuts at: 679 1243 679
Size: 564 3767
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 736 736
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 674 1122 674
Size: 448 3883
PspOMI G'GGCC_C
Cuts at: 1263 1263
Size: 4331
PstI C_TGCA'G
Cuts at: 926 1378 2063 926
Size: 452 685 3194
Fragments arranged by size:
3194 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 283 306 952 1756 2116 283
Size: 23 646 804 360 2498
Fragments arranged by size:
2498 804 646 360 23
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 214 1345 214
Size: 1131 3200
SalI G'TCGA_C
Cuts at: 748 1368 748
Size: 620 3711
SanDI GG'GwC_CC
Cuts at: 674 1122 674
Size: 448 3883
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SexAI A'CCwGG_T
Cuts at: 496 496
Size: 4331
SfcI C'TryA_G
Cuts at: 33 922 964 1374 2059 3052 3243 33
Size: 889 42 410 685 993 191 1121
Fragments arranged by size:
1121 993 889 685 410 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 1245 1245
Size: 4331
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SgrAI Cr'CCGG_yG
Cuts at: 812 812
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 1486 2401 2893 3155 1486
Size: 915 492 262 2662
Fragments arranged by size:
2662 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SspI AAT'ATT
Cuts at: 582 582
Size: 4331
StyI C'CwwG_G
Cuts at: 713 1465 1819 713
Size: 752 354 3225
Fragments arranged by size:
3225 752 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 743 1096 1914 2047 2506 2715 743
Size: 353 818 133 459 209 2359
Fragments arranged by size:
2359 818 459 353 209 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 643 733 1826 643
Size: 90 1093 3148
Fragments arranged by size:
3148 1093 90
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
ZraI GAC'GTC
Cuts at: 192 192
Size: 4331
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SnaBI
SrfI StuI SwaI XhoI XmnI