Restriction map for pF1KE2363
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflIII      AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AviII       BamHI       BbvCI       BcgI        BciVI       
BclI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        Ksp632I     KspI        MfeI        
MluI        MroI        MunI        MvnI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          349  GTCTAC               GT^[AC][GT]AC
AcyI          278  GGCGCC               G[GA]^CG[CT]C
AflII        1495  CTTAAG               C^TTAAG
AgeI          781  ACCGGT               A^CCGGT
AlwNI        1264  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1078  CCTCAGG              CC^T[ACGT]AGG
AvrII        1110  CCTAGG               C^CTAGG
BbeI          278  GGCGCC               GGCGC^C
BbrPI         199  CACGTG               CAC^GTG
BbsI          153  GAAGAC               GAAGAC(2/6)
BfrI         1495  CTTAAG               C^TTAAG
BglI           32  GCCTCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1247  AGATCT               A^GATCT
BlnI         1110  CCTAGG               C^CTAGG
BlpI           27  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I       27  GCTTAGC              GC^T[ACGT]AGC
BpuAI         153  GAAGAC               GAAGAC(2/6)
BsaAI         199  CACGTG               [CT]AC^GT[GA]
BsaBI         223  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         278  GGCGCC               G[GA]^CG[CT]C
BsaI          367  GAGACC               GGTCTC(1/5)
BsaWI         781  ACCGGT               [AT]^CCGG[AT]
BsmAI         367  GAGAC                GTCTC(1/5)
BsrI          948  CCAGT                ACTGG(1/-1)
Bsu36I       1078  CCTCAGG              CC^T[ACGT]AGG
CelII          27  GCTTAGC              GC^T[ACGT]AGC
Eco81I       1078  CCTCAGG              CC^T[ACGT]AGG
EcoNI         669  CCTCATCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         278  GGCGCC               [GA]GCGC^[CT]
Hin1I         278  GGCGCC               G[GA]^CG[CT]C
KasI          278  GGCGCC               G^GCGCC
MaeII         200  ACGT                 A^CGT
MamI          223  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          305  GCCGGC               GCC^GGC
NarI          278  GGCGCC               GG^CGCC
NgoMIV        305  GCCGGC               G^CCGGC
NspI          903  GCATGC               [GA]CATG^[CT]
PflMI         750  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         781  ACCGGT               A^CCGGT
PmaCI         199  CACGTG               CAC^GTG
PmlI          199  CACGTG               CAC^GTG
PpuMI         268  GGGTCCC              [GA]G^G[AT]CC[CT]
SexAI        1318  ACCAGGT              A^CC[AT]GGT
SfoI          278  GGCGCC               GGC^GCC
SphI          903  GCATGC               GCATG^C
StuI         1499  AGGCCT               AGG^CCT
Tsp509I      1233  AATT                 ^AATT
Van91I        750  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AfaI          441  GTAC                 GT^AC
             1371  GTAC                
Asp700I       153  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              384  GAAGCCTTTC          
BanI           15  GGCACC               G^G[CT][GA]CC
              278  GGCGCC              
BanII         520  GAGCCC               G[GA]GC[CT]^C
              867  GAGCCC              
BfaI          331  CTAG                 C^TAG
             1111  CTAG                
BsgI         1216  GTGCAG               GTGCAG(16/14)
             1261  GTGCAG              
BsmFI        1396  GGGAC                GGGAC(10/14)
              270  GTCCC               
BspMI        1429  ACCTGC               ACCTGC(4/8)
              733  GCAGGT              
BsrBI         298  GAGCGG               CCGCTC(-3/-3)
              337  GAGCGG              
BsrFI         305  GCCGGC               [GA]^CCGG[CT]
              781  ACCGGT              
BstEII        211  GGTGACC              G^GT[ACGT]ACC
             1521  GGTCACC             
BstPI         211  GGTGACC              G^GT[ACGT]ACC
             1521  GGTCACC             
BstXI         479  CCATCCCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              788  CCACCGAGGTGG        
BstYI        1247  AGATCT               [GA]^GATC[CT]
             1353  GGATCT              
Cfr10I        305  GCCGGC               [GA]^CCGG[CT]
              781  ACCGGT              
DraII         268  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1074  GGGGCCT             
EcoO109I      268  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1074  GGGGCCT             
EcoO65I       211  GGTGACC              G^GT[ACGT]ACC
             1521  GGTCACC             
HincII        169  GTCAAC               GT[CT]^[GA]AC
              763  GTTGAC              
HindII        169  GTCAAC               GT[CT]^[GA]AC
              763  GTTGAC              
MaeI          331  CTAG                 C^TAG
             1111  CTAG                
MflI         1247  AGATCT               [GA]^GATC[CT]
             1353  GGATCT              
MseI         1454  TTAA                 T^TAA
             1496  TTAA                
MspA1I        112  CAGCTG               C[AC]G^C[GT]G
              818  CAGCTG              
NcoI          274  CCATGG               C^CATGG
              485  CCATGG              
PaeR7I        364  CTCGAG               C^TCGAG
             1006  CTCGAG              
PvuII         112  CAGCTG               CAG^CTG
              818  CAGCTG              
RsaI          441  GTAC                 GT^AC
             1371  GTAC                
SmaI          248  CCCGGG               CCC^GGG
             1349  CCCGGG              
Tru9I        1454  TTAA                 T^TAA
             1496  TTAA                
XhoI          364  CTCGAG               C^TCGAG
             1006  CTCGAG              
XhoII        1247  AGATCT               [GA]^GATC[CT]
             1353  GGATCT              
XmaCI         248  CCCGGG               C^CCGGG
             1349  CCCGGG              
XmaI          248  CCCGGG               C^CCGGG
             1349  CCCGGG              
XmnI          153  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              384  GAAGCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BalI        BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsoBI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        
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