Restriction map for pF1KB5403
 (Linear) (Six-base) MAPSORT of: KIBB5403.seq  Check: 3772  from: 1  to: 2739



KIBB5403.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2030    2739 
   Size:       2030     709
 
AccI GT'mk_AC

Cuts at:      0    2054    2739 
   Size:       2054     685
 
Acc65I G'GTAC_C

Cuts at:      0    1745    2739 
   Size:       1745     994
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527    2739 
   Size:        527    2212
 
AfeI AGC'GCT

Cuts at:      0    1462    2739 
   Size:       1462    1277
 
AflIII A'CryG_T

Cuts at:      0    1696    2205    2426    2739 
   Size:       1696     509     221     313

  Fragments arranged by size: 

                1696     509     313     221
 
AgeI A'CCGG_T

Cuts at:      0      19    2739 
   Size:         19    2720
 
AhdI GACnn_n'nnGTC

Cuts at:      0      33    1908    2739 
   Size:         33    1875     831

  Fragments arranged by size: 

                1875     831      33
 
AleI CACnn'nnGTG

Cuts at:      0    1837    2739 
   Size:       1837     902
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1525    1855    2739 
   Size:       1525     330     884

  Fragments arranged by size: 

                1525     884     330
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1311    1947    2175    2562    2739 
   Size:       1311     636     228     387     177

  Fragments arranged by size: 

                1311     636     387     228     177
 
ApaLI G'TGCA_C

Cuts at:      0    2452    2739 
   Size:       2452     287
 
ApoI r'AATT_y

Cuts at:      0    1360    2739 
   Size:       1360    1379
 
AseI AT'TA_AT

Cuts at:      0    2480    2739 
   Size:       2480     259
 
AvaI C'yCGr_G

Cuts at:      0     487    2308    2739 
   Size:        487    1821     431

  Fragments arranged by size: 

                1821     487     431
 
BamHI G'GATC_C

Cuts at:      0    1688    2739 
   Size:       1688    1051
 
BanI G'GyrC_C

Cuts at:      0     777    1745    2000    2635    2739 
   Size:        777     968     255     635     104

  Fragments arranged by size: 

                 968     777     635     255     104
 
BanII G_rGCy'C

Cuts at:      0      65     254    1585    2546    2739 
   Size:         65     189    1331     961     193

  Fragments arranged by size: 

                1331     961     193     189      65
 
BbeI G_GCGC'C

Cuts at:      0    2004    2739 
   Size:       2004     735
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     692    2254    2739 
   Size:        692    1562     485

  Fragments arranged by size: 

                1562     692     485
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478     512    2739 
   Size:        478      34    2227

  Fragments arranged by size: 

                2227     478      34
 
BclI T'GATC_A

Cuts at:      0    2126    2474    2739 
   Size:       2126     348     265

  Fragments arranged by size: 

                2126     348     265
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2224    2705    2739 
   Size:       2224     481      34

  Fragments arranged by size: 

                2224     481      34
 
BlpI GC'TnA_GC

Cuts at:      0     112    2739 
   Size:        112    2627
 
Bme1580I G_kGCm'C

Cuts at:      0    1483    2456    2638    2739 
   Size:       1483     973     182     101

  Fragments arranged by size: 

                1483     973     182     101
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     699    2739 
   Size:        699    2040
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1602    2739 
   Size:       1602    1137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1489    2056    2065    2098    2739 
   Size:       1489     567       9      33     641

  Fragments arranged by size: 

                1489     641     567      33       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     744    2739 
   Size:        744    1995
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2651    2739 
   Size:       2651      88
 
BsaAI yAC'GTr

Cuts at:      0    1407    2739 
   Size:       1407    1332
 
BsaBI GATnn'nnATC

Cuts at:      0    1228    2739 
   Size:       1228    1511
 
BsaHI Gr'CG_yC

Cuts at:      0    1106    2001    2739 
   Size:       1106     895     738

  Fragments arranged by size: 

                1106     895     738
 
BsaWI w'CCGG_w

Cuts at:      0      19     191    1685    2739 
   Size:         19     172    1494    1054

  Fragments arranged by size: 

                1494    1054     172      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1091    1121    1378    1408    1636    1666    2404    2434 
   Size:       1091      30     257      30     228      30     738      30

Cuts at:   2434    2739 
   Size:        305

  Fragments arranged by size: 

                1091     738     305     257     228      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     187     538     742     886     889     892    1486 
   Size:         73     114     351     204     144       3       3     594

Cuts at:   1486    1504    1575    2500    2739 
   Size:         18      71     925     239

  Fragments arranged by size: 

                 925     594     351     239     204     144     114      73
                  71      18       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1845    2178    2552    2586    2739 
   Size:       1845     333     374      34     153

  Fragments arranged by size: 

                1845     374     333     153      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1276    1477    1507    1717    1834    1861    2008    2739 
   Size:       1276     201      30     210     117      27     147     731

  Fragments arranged by size: 

                1276     731     210     201     147     117      30      27
            
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     254     418    1415    2456    2739 
   Size:         65     189     164     997    1041     283

  Fragments arranged by size: 

                1041     997     283     189     164      65
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2407    2739 
   Size:       2407     332
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     254     418    1415    1483    1585    2456    2546 
   Size:         65     189     164     997      68     102     871      90

Cuts at:   2546    2638    2739 
   Size:         92     101

  Fragments arranged by size: 

                 997     871     189     164     102     101      92      90
                  68      65
 
BspEI T'CCGG_A

Cuts at:      0     191    2739 
   Size:        191    2548
 
BspHI T'CATG_A

Cuts at:      0    1769    2739 
   Size:       1769     970
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1298    1517    2030    2739 
   Size:       1298     219     513     709

  Fragments arranged by size: 

                1298     709     513     219
 
BsrBI CCG'CTC

Cuts at:      0    1998    2739 
   Size:       1998     741
 
BsrFI r'CCGG_y

Cuts at:      0      19    2739 
   Size:         19    2720
 
BssSI C'ACGA_G

Cuts at:      0    1016    2739 
   Size:       1016    1723
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     864    1190    1915    2076    2235    2409    2739 
   Size:        237     627     326     725     161     159     174     330

  Fragments arranged by size: 

                 725     627     330     326     237     174     161     159
            
 
BstYI r'GATC_y

Cuts at:      0     431    1004    1688    1817    2081    2345    2439    2739 
   Size:        431     573     684     129     264     264      94     300

  Fragments arranged by size: 

                 684     573     431     300     264     264     129      94
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1577    2739 
   Size:       1577    1162
 
BtgI C'CryG_G

Cuts at:      0    1320    1835    2700    2739 
   Size:       1320     515     865      39

  Fragments arranged by size: 

                1320     865     515      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1119    2123    2739 
   Size:       1119    1004     616

  Fragments arranged by size: 

                1119    1004     616
 
BtsI GCAGTG_nn'

Cuts at:      0     156    1309    2394    2546    2739 
   Size:        156    1153    1085     152     193

  Fragments arranged by size: 

                1153    1085     193     156     152
 
DraIII CAC_nnn'GTG

Cuts at:      0     233    1315    2739 
   Size:        233    1082    1424

  Fragments arranged by size: 

                1424    1082     233
 
EaeI y'GGCC_r

Cuts at:      0    1917    2093    2576    2739 
   Size:       1917     176     483     163

  Fragments arranged by size: 

                1917     483     176     163
 
EarI CTCTTCn'nnn_

Cuts at:      0     236     546    1271    2348    2380    2739 
   Size:        236     310     725    1077      32     359

  Fragments arranged by size: 

                1077     725     359     310     236      32
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     932    1852    2739 
   Size:        932     920     887

  Fragments arranged by size: 

                 932     920     887
 
EcoICRI GAG'CTC

Cuts at:      0      63     252    2739 
   Size:         63     189    2487

  Fragments arranged by size: 

                2487     189      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527    1489    2056    2065    2098    2739 
   Size:        527     962     567       9      33     641

  Fragments arranged by size: 

                 962     641     567     527      33       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      80     868    1099    1876    2739 
   Size:         80     788     231     777     863

  Fragments arranged by size: 

                 863     788     777     231      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1944    2010    2182    2298    2366    2739 
   Size:       1944      66     172     116      68     373

  Fragments arranged by size: 

                1944     373     172     116      68      66
 
EcoRV GAT'ATC

Cuts at:      0    1230    2739 
   Size:       1230    1509
 
FspI TGC'GCA

Cuts at:      0    1516    1957    2739 
   Size:       1516     441     782

  Fragments arranged by size: 

                1516     782     441
 
HaeII r_GCGC'y

Cuts at:      0     864    1464    2004    2739 
   Size:        864     600     540     735

  Fragments arranged by size: 

                 864     735     600     540
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      25      51      57     442     474     646     678 
   Size:         19       6      26       6     385      32     172      32

Cuts at:    678     706     738     838     870    1091    1123    1378    1410 
   Size:         28      32     100      32     221      32     255      32

Cuts at:   1410    1894    1926    2111    2143    2404    2436    2739 
   Size:        484      32     185      32     261      32     303

  Fragments arranged by size: 

                 484     385     303     261     255     221     185     172
                 100      32      32      32      32      32      32      32
                  32      32      28      26      19       6       6
 
KasI G'GCGC_C

Cuts at:      0    2000    2739 
   Size:       2000     739
 
KpnI G_GTAC'C

Cuts at:      0    1749    2739 
   Size:       1749     990
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     743    1239    2739 
   Size:         60     683     496    1500

  Fragments arranged by size: 

                1500     683     496      60
 
MslI CAynn'nnrTG

Cuts at:      0    1768    1837    2233    2407    2739 
   Size:       1768      69     396     174     332

  Fragments arranged by size: 

                1768     396     332     174      69
 
MspA1I CmG'CkG

Cuts at:      0    1084    1566    1845    2151    2574    2702    2739 
   Size:       1084     482     279     306     423     128      37

  Fragments arranged by size: 

                1084     482     423     306     279     128      37
 
NarI GG'CG_CC

Cuts at:      0    2001    2739 
   Size:       2001     738
 
NcoI C'CATG_G

Cuts at:      0    1835    2739 
   Size:       1835     904
 
NdeI CA'TA_TG

Cuts at:      0     267    2739 
   Size:        267    2472
 
NspI r_CATG'y

Cuts at:      0    1700    1801    2209    2430    2697    2739 
   Size:       1700     101     408     221     267      42

  Fragments arranged by size: 

                1700     408     267     221     101      42
 
PciI A'CATG_T

Cuts at:      0    1696    2205    2426    2739 
   Size:       1696     509     221     313

  Fragments arranged by size: 

                1696     509     313     221
 
PfoI T'CCnGG_A

Cuts at:      0    2075    2348    2739 
   Size:       2075     273     391

  Fragments arranged by size: 

                2075     391     273
 
PpuMI rG'GwC_Cy

Cuts at:      0    2010    2182    2298    2366    2739 
   Size:       2010     172     116      68     373

  Fragments arranged by size: 

                2010     373     172     116      68
 
PstI C_TGCA'G

Cuts at:      0    1967    2573    2739 
   Size:       1967     606     166

  Fragments arranged by size: 

                1967     606     166
 
PvuII CAG'CTG

Cuts at:      0    1566    1845    2151    2574    2739 
   Size:       1566     279     306     423     165

  Fragments arranged by size: 

                1566     423     306     279     165
 
SacI G_AGCT'C

Cuts at:      0      65     254    2739 
   Size:         65     189    2485

  Fragments arranged by size: 

                2485     189      65
 
SacII CC_GC'GG

Cuts at:      0    2703    2739 
   Size:       2703      36
 
SanDI GG'GwC_CC

Cuts at:      0    2182    2739 
   Size:       2182     557
 
SapI GCTCTTCn'nnn_

Cuts at:      0     546    2739 
   Size:        546    2193
 
SbfI CC_TGCA'GG

Cuts at:      0    1967    2739 
   Size:       1967     772
 
SexAI A'CCwGG_T

Cuts at:      0    2057    2739 
   Size:       2057     682
 
SfcI C'TryA_G

Cuts at:      0    1009    1963    2382    2569    2739 
   Size:       1009     954     419     187     170

  Fragments arranged by size: 

                1009     954     419     187     170
 
SfoI GGC'GCC

Cuts at:      0    2002    2739 
   Size:       2002     737
 
SmlI C'TyrA_G

Cuts at:      0     759    2739 
   Size:        759    1980
 
SphI G_CATG'C

Cuts at:      0    1801    2739 
   Size:       1801     938
 
SspI AAT'ATT

Cuts at:      0    1269    2739 
   Size:       1269    1470
 
StuI AGG'CCT

Cuts at:      0    1871    2739 
   Size:       1871     868
 
StyI C'CwwG_G

Cuts at:      0     155     404     956    1670    1835    1866    1877    1925 
   Size:        155     249     552     714     165      31      11      48

Cuts at:   1925    2222    2739 
   Size:        297     517

  Fragments arranged by size: 

                 714     552     517     297     249     165     155      48
                  31      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     795     988    2227    2739 
   Size:        795     193    1239     512

  Fragments arranged by size: 

                1239     795     512     193
 
TatI w'GTAC_w

Cuts at:      0    1658    2739 
   Size:       1658    1081
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1301    1333    1636    1668    2739 
   Size:       1301      32     303      32    1071

  Fragments arranged by size: 

                1301    1071     303      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     568    2320    2418    2739 
   Size:        568    1752      98     321

  Fragments arranged by size: 

                1752     568     321      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285    1842    1932    2739 
   Size:        285    1557      90     807

  Fragments arranged by size: 

                1557     807     285      90
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI     BclI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BssSI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII
    Hin4I     KasI     KpnI     MmeI     MslI   MspA1I     NarI     NcoI
     NdeI     NspI     PciI     PfoI    PpuMI     PstI    PvuII     SacI
    SacII    SanDI     SapI     SbfI    SexAI     SfcI     SfoI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AloI     ApaI     AscI    AsiSI    AvrII
     BaeI    BbvCI    BciVI    BfrBI    BglII    BmgBI     BmtI   Bpu10I
    BsiEI    BsiWI     BsmI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI    EcoRI
     FalI     FseI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     MscI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI     ScaI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     XbaI     XhoI
     XmaI     XmnI     ZraI
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