Restriction map for pF1KB3692
 (Linear) (Six-base) MAPSORT of: KIBB3692.seq  Check: 335  from: 1  to: 384



KIBB3692.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:      0     224     384 
   Size:        224     160
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45     384 
   Size:         45     339
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     384 
   Size:        255     129
 
BsaHI Gr'CG_yC

Cuts at:      0     221     384 
   Size:        221     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35     384 
   Size:          5      30     349

  Fragments arranged by size: 

                 349      30       5
 
BseYI C'CCAG_C

Cuts at:      0     166     384 
   Size:        166     218
 
BsrDI GCAATG_nn'

Cuts at:      0     204     384 
   Size:        204     180
 
BtsI GCAGTG_nn'

Cuts at:      0      85     384 
   Size:         85     299
 
EcoO109I rG'GnC_Cy

Cuts at:      0     273     384 
   Size:        273     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37     363     384 
   Size:          5      32     326      21

  Fragments arranged by size: 

                 326      32      21       5
 
MfeI C'AATT_G

Cuts at:      0     126     384 
   Size:        126     258
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     384 
   Size:        172     212
 
PpuMI rG'GwC_Cy

Cuts at:      0     273     384 
   Size:        273     111
 
PstI C_TGCA'G

Cuts at:      0      80     384 
   Size:         80     304
 
SfcI C'TryA_G

Cuts at:      0      76     384 
   Size:         76     308
 
SmlI C'TyrA_G

Cuts at:      0     234     384 
   Size:        234     150
 
StyI C'CwwG_G

Cuts at:      0       9     384 
   Size:          9     375
 
ZraI GAC'GTC

Cuts at:      0     222     384 
   Size:        222     162
 

 Enzymes that do cut:

    AatII     BbsI    BpuEI    BsaHI    BsaXI    BseYI    BsrDI     BtsI
 EcoO109I    Hin4I     MfeI     MmeI    PpuMI     PstI     SfcI     SmlI
     StyI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI
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