Restriction map for pF1KB6301
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BseRI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        TseI        
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI          232  GTCTAC               GT^[AC][GT]AC
AciI          251  GCGG                 CCGC(-3/-1)
AcsI          704  GAATTT               [GA]^AATT[CT]
ApoI          704  GAATTT               [GA]^AATT[CT]
Asp700I       585  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          136  CCCGGG               C^[CT]CG[GA]G
BglI          314  GCCCCTATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         292  ACCGGA               [AT]^CCGG[AT]
BsgI          221  CTGCAC               GTGCAG(16/14)
BsoBI         136  CCCGGG               C^[CT]CG[GA]G
BstF5I         61  GGATG                GGATG(2/0)
BstXI          84  CCAGCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Fnu4HI        251  GCGGC                GC^[ACGT]GC
FokI           61  GGATG                GGATG(9/13)
ItaI          251  GCGGC                GC^[ACGT]GC
MaeIII        713  GTGAC                ^GT[ACGT]AC
MspA1I        249  CAGCGG               C[AC]G^C[GT]G
NspI          386  GCATGC               [GA]CATG^[CT]
PflMI         268  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI          603  AGTACT               AGT^ACT
SmaI          136  CCCGGG               CCC^GGG
SmlI           56  CTCAAG               C^T[CT][GA]AG
SphI          386  GCATGC               GCATG^C
Tsp45I        713  GTGAC                ^GT[GC]AC
Van91I        268  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         136  CCCGGG               C^CCGGG
XmaI          136  CCCGGG               C^CCGGG
XmnI          585  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          181  GGCGCC               G[GA]^CG[CT]C
              322  GGCGCC              
AfaI          604  GTAC                 GT^AC
              660  GTAC                
AluI          204  AGCT                 AG^CT
              527  AGCT                
AspHI         194  GAGCAC               G[AT]GC[AT]^C
              215  GAGCAC              
BbeI          181  GGCGCC               GGCGC^C
              322  GGCGCC              
BsaHI         181  GGCGCC               G[GA]^CG[CT]C
              322  GGCGCC              
BsiHKAI       194  GAGCAC               G[AT]GC[AT]^C
              215  GAGCAC              
BspMI         219  ACCTGC               ACCTGC(4/8)
              356  ACCTGC              
DraII         127  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              259  GGGGCCT             
EcoO109I      127  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              259  GGGGCCT             
HaeII         181  GGCGCC               [GA]GCGC^[CT]
              322  GGCGCC              
Hin1I         181  GGCGCC               G[GA]^CG[CT]C
              322  GGCGCC              
HinfI         230  GAGTC                G^A[ACGT]TC
              506  GAGTC               
KasI          181  GGCGCC               G^GCGCC
              322  GGCGCC              
MboII         550  GAAGA                GAAGA(8/7)
              271  TCTTC               
MseI          476  TTAA                 T^TAA
              674  TTAA                
NarI          181  GGCGCC               GG^CGCC
              322  GGCGCC              
PleI          230  GAGTC                GAGTC(4/5)
              506  GAGTC               
RsaI          604  GTAC                 GT^AC
              660  GTAC                
SfaNI          25  GATGC                GCATC(5/9)
              643  GATGC               
SfoI          181  GGCGCC               GGC^GCC
              322  GGCGCC              
Tru9I         476  TTAA                 T^TAA
              674  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

BanI        BcnI        BmyI        BpmI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        MnlI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com