Restriction map for pF1KE0203
 (Linear) (Six-base) MAPSORT of: KIEE0203.seq  Check: 3921  from: 1  to: 903



KIEE0203.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     903 
   Size:        311     592
 
AflII C'TTAA_G

Cuts at:      0     240     903 
   Size:        240     663
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     887     903 
   Size:        887      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169     903 
   Size:        137      32     734

  Fragments arranged by size: 

                 734     137      32
 
ApoI r'AATT_y

Cuts at:      0     265     425     903 
   Size:        265     160     478

  Fragments arranged by size: 

                 478     265     160
 
AseI AT'TA_AT

Cuts at:      0     214     903 
   Size:        214     689
 
AvrII C'CTAG_G

Cuts at:      0     672     903 
   Size:        672     231
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     776     903 
   Size:        299     477     127

  Fragments arranged by size: 

                 477     299     127
 
Bme1580I G_kGCm'C

Cuts at:      0     333     708     903 
   Size:        333     375     195

  Fragments arranged by size: 

                 375     333     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     336     903 
   Size:        336     567
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68      77     903 
   Size:         68       9     826

  Fragments arranged by size: 

                 826      68       9
 
BsaBI GATnn'nnATC

Cuts at:      0     307     737     903 
   Size:        307     430     166

  Fragments arranged by size: 

                 430     307     166
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      54      84     137     167     422     452     903 
   Size:         54      30      53      30     255      30     451

  Fragments arranged by size: 

                 451     255      54      53      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     763     903 
   Size:        763     140
 
BsiHKAI G_wGCw'C

Cuts at:      0     150     326     903 
   Size:        150     176     577

  Fragments arranged by size: 

                 577     176     150
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     326     333     708     903 
   Size:        150     176       7     375     195

  Fragments arranged by size: 

                 375     195     176     150       7
 
BspHI T'CATG_A

Cuts at:      0     282     903 
   Size:        282     621
 
BstYI r'GATC_y

Cuts at:      0     522     903 
   Size:        522     381
 
BtsI GCAGTG_nn'

Cuts at:      0     829     903 
   Size:        829      74
 
DraI TTT'AAA

Cuts at:      0     419     815     849     903 
   Size:        419     396      34      54

  Fragments arranged by size: 

                 419     396      54      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     584     713     815     903 
   Size:        125     459     129     102      88

  Fragments arranged by size: 

                 459     129     125     102      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68      77     311     903 
   Size:         68       9     234     592

  Fragments arranged by size: 

                 592     234      68       9
 
EcoRI G'AATT_C

Cuts at:      0     425     903 
   Size:        425     478
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     422     454     648     680     862     894 
   Size:        118      32     272      32     194      32     182      32

Cuts at:    894     903 
   Size:          9

  Fragments arranged by size: 

                 272     194     182     118      32      32      32      32
                   9
 
NspI r_CATG'y

Cuts at:      0      52     250     903 
   Size:         52     198     653

  Fragments arranged by size: 

                 653     198      52
 
PasI CC'CwG_GG

Cuts at:      0     701     903 
   Size:        701     202
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169     903 
   Size:        137      32     734

  Fragments arranged by size: 

                 734     137      32
 
PstI C_TGCA'G

Cuts at:      0     356     903 
   Size:        356     547
 
SapI GCTCTTCn'nnn_

Cuts at:      0     584     903 
   Size:        584     319
 
SfcI C'TryA_G

Cuts at:      0     352     903 
   Size:        352     551
 
SmlI C'TyrA_G

Cuts at:      0     240     903 
   Size:        240     663
 
SspI AAT'ATT

Cuts at:      0     888     903 
   Size:        888      15
 
StyI C'CwwG_G

Cuts at:      0     629     672     903 
   Size:        629      43     231

  Fragments arranged by size: 

                 629     231      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     714     903 
   Size:        714     189
 
XmnI GAAnn'nnTTC

Cuts at:      0      65     773     903 
   Size:         65     708     130

  Fragments arranged by size: 

                 708     130      65
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     AloI     ApoI     AseI    AvrII     BbsI
 Bme1580I     BmrI     BpmI    BsaBI    BsaXI    BseRI  BsiHKAI Bsp1286I
    BspHI    BstYI     BtsI     DraI     EarI  Eco57MI    EcoRI    Hin4I
     NspI     PasI     PpiI     PstI     SapI     SfcI     SmlI     SspI
     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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