Restriction map for pF1KB5454
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsiHKAI     BsiWI       BsmI        
BspDI       BspEI       BspLU11I    BssHII      BssSI       
Bst1107I    BstAPI      BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        Eco81I      
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NarI        NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SgrAI       SmlI        SnaBI       SphI        
Sse8387I    SwaI        Tru9I       Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AatII        1495  GACGTC               GACGT^C
AccI          891  GTCTAC               GT^[AC][GT]AC
AccII         354  CGCG                 CG^CG
AcyI         1495  GACGTC               G[GA]^CG[CT]C
AhdI         1000  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1057  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        74  AGCGCT               AGC^GCT
AspEI        1000  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           87  CCCGGG               C^[CT]CG[GA]G
BcgI          182  GCAAGACAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          556  GCCGTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          717  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      717  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1495  GACGTC               G[GA]^CG[CT]C
BsaI         1176  GGTCTC               GGTCTC(1/5)
BsiEI         856  CGGCCG               CG[GA][CT]^CG
BsoBI          87  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      484  TGTACA               T^GTACA
BspHI         683  TCATGA               T^CATGA
BsrDI         825  CATTGC               GCAATG(2/0)
BsrFI        1211  GCCGGC               [GA]^CCGG[CT]
BsrGI         484  TGTACA               T^GTACA
BstBI        1217  TTCGAA               TT^CGAA
BstEII       1530  GGTCACC              G^GT[ACGT]ACC
BstPI        1530  GGTCACC              G^GT[ACGT]ACC
BstUI         354  CGCG                 CG^CG
BstYI           9  GGATCT               [GA]^GATC[CT]
CelII         717  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1211  GCCGGC               [GA]^CCGG[CT]
DraIII       1078  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          856  CGGCCG               C^GGCCG
Eam1105I     1000  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1024  GAAGAG               CTCTTC(1/4)
EclXI         856  CGGCCG               C^GGCCG
Eco47III       74  AGCGCT               AGC^GCT
Eco52I        856  CGGCCG               C^GGCCG
EcoNI         606  CCTGGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1530  GGTCACC              G^GT[ACGT]ACC
HaeII          74  AGCGCT               [GA]GCGC^[CT]
Hin1I        1495  GACGTC               G[GA]^CG[CT]C
HindIII      1126  AAGCTT               A^AGCTT
Ksp632I      1024  GAAGAG               CTCTTC(1/4)
MaeII        1496  ACGT                 A^CGT
MflI            9  GGATCT               [GA]^GATC[CT]
MspA1I        103  CAGCTG               C[AC]G^C[GT]G
MvnI          354  CGCG                 CG^CG
NaeI         1211  GCCGGC               GCC^GGC
NcoI          921  CCATGG               C^CATGG
NdeI         1270  CATATG               CA^TATG
NgoMIV       1211  GCCGGC               G^CCGGC
NspV         1217  TTCGAA               TT^CGAA
PflMI        1056  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         103  CAGCTG               CAG^CTG
RcaI          683  TCATGA               T^CATGA
ScaI          500  AGTACT               AGT^ACT
SexAI         515  ACCTGGT              A^CC[AT]GGT
SfuI         1217  TTCGAA               TT^CGAA
SmaI           87  CCCGGG               CCC^GGG
SpeI           93  ACTAGT               A^CTAGT
SspBI         484  TGTACA               T^GTACA
SspI         1087  AATATT               AAT^ATT
StuI         1139  AGGCCT               AGG^CCT
Van91I       1056  CCAGTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1238  CCAAGAAGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           9  GGATCT               [GA]^GATC[CT]
XmaCI          87  CCCGGG               C^CCGGG
XmaI           87  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI         1401  GATCC                GGATC(4/5)
                9  GGATC               
Asp700I        72  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1368  GAAGGAGTTC          
BanII         915  GGGCTC               G[GA]GC[CT]^C
             1152  GGGCTC              
BfaI           94  CTAG                 C^TAG
             1504  CTAG                
BmyI          915  GGGCTC               G[AGT]GC[ACT]^C
             1152  GGGCTC              
BpmI         1135  CTGGAG               CTGGAG(16/14)
             1321  CTGGAG              
BseRI         322  CTCCTC               GAGGAG(10/8)
             1045  GAGGAG              
BsgI          415  CTGCAC               GTGCAG(16/14)
              835  CTGCAC              
BsmBI          61  GAGACG               CGTCTC(1/5)
             1493  GAGACG              
Bsp1286I      915  GGGCTC               G[AGT]GC[ACT]^C
             1152  GGGCTC              
BspMI         866  ACCTGC               ACCTGC(4/8)
              895  ACCTGC              
BsrBI         358  GAGCGG               CCGCTC(-3/-3)
              379  GAGCGG              
DpnII          10  GATC                 ^GATC
             1401  GATC                
EaeI          856  CGGCCG               [CT]^GGCC[GA]
              924  TGGCCG              
EcoT14I       641  CCTTGG               C^C[AT][AT]GG
              921  CCATGG              
MaeI           94  CTAG                 C^TAG
             1504  CTAG                
MboI           10  GATC                 ^GATC
             1401  GATC                
NdeII          10  GATC                 ^GATC
             1401  GATC                
PstI          272  CTGCAG               CTGCA^G
             1516  CTGCAG              
Sau3AI         10  GATC                 ^GATC
             1401  GATC                
SfcI          272  CTGCAG               C^T[GA][CT]AG
             1516  CTGCAG              
StyI          641  CCTTGG               C^C[AT][AT]GG
              921  CCATGG              
Tsp509I      1189  AATT                 ^AATT
             1364  AATT                
XmnI           72  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1368  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
DsaI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau96I      ScrFI       SfaNI       
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com