Restriction map for pF1KE2300
 (Linear) (Six-base) MAPSORT of: KIEE2300.seq  Check: 7082  from: 1  to: 2571



KIEE2300.orf

 With 182 enzymes: SgfI * 

                        November 10, 2010 10:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1437    2571 
   Size:       1437    1134
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1520    2438    2571 
   Size:       1087     433     918     133

  Fragments arranged by size: 

                1087     918     433     133
 
AfeI AGC'GCT

Cuts at:      0     290    2571 
   Size:        290    2281
 
AleI CACnn'nnGTG

Cuts at:      0    2507    2571 
   Size:       2507      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1563    2571 
   Size:       1563    1008
 
ApoI r'AATT_y

Cuts at:      0     119     332     421     555     603     768     975    1512 
   Size:        119     213      89     134      48     165     207     537

Cuts at:   1512    1568    2263    2375    2457    2571 
   Size:         56     695     112      82     114

  Fragments arranged by size: 

                 695     537     213     207     165     134     119     114
                 112      89      82      56      48
 
AseI AT'TA_AT

Cuts at:      0    2213    2571 
   Size:       2213     358
 
AvaI C'yCGr_G

Cuts at:      0     144    2571 
   Size:        144    2427
 
BanII G_rGCy'C

Cuts at:      0    2354    2571 
   Size:       2354     217
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      95     865    1081    2087    2571 
   Size:         95     770     216    1006     484

  Fragments arranged by size: 

                1006     770     484     216      95
 
BfrBI ATG'CAT

Cuts at:      0    1550    2571 
   Size:       1550    1021
 
BglII A'GATC_T

Cuts at:      0    2286    2571 
   Size:       2286     285
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     817    2571 
   Size:        817    1754
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1388    2085    2571 
   Size:       1388     697     486

  Fragments arranged by size: 

                1388     697     486
 
Bpu10I CC'TnA_GC

Cuts at:      0    2348    2571 
   Size:       2348     223
 
BsaAI yAC'GTr

Cuts at:      0     385    2571 
   Size:        385    2186
 
BsaBI GATnn'nnATC

Cuts at:      0    2249    2571 
   Size:       2249     322
 
BsaWI w'CCGG_w

Cuts at:      0     646     764    1227    1703    2571 
   Size:        646     118     463     476     868

  Fragments arranged by size: 

                 868     646     476     463     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604    2571 
   Size:        604    1967
 
BsiHKAI G_wGCw'C

Cuts at:      0    2354    2571 
   Size:       2354     217
 
BsmI GAATG_Cn'

Cuts at:      0     919    1552    1979    2571 
   Size:        919     633     427     592

  Fragments arranged by size: 

                 919     633     592     427
 
Bsp1286I G_dGCh'C

Cuts at:      0    2354    2571 
   Size:       2354     217
 
BspEI T'CCGG_A

Cuts at:      0    1227    2571 
   Size:       1227    1344
 
BspHI T'CATG_A

Cuts at:      0    2253    2571 
   Size:       2253     318
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1437    1470    2571 
   Size:       1437      33    1101

  Fragments arranged by size: 

                1437    1101      33
 
BstEII G'GTnAC_C

Cuts at:      0     760    2571 
   Size:        760    1811
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     191    1921    2449    2571 
   Size:        191    1730     528     122

  Fragments arranged by size: 

                1730     528     191     122
 
BstYI r'GATC_y

Cuts at:      0     937    2286    2571 
   Size:        937    1349     285

  Fragments arranged by size: 

                1349     937     285
 
Bsu36I CC'TnA_GG

Cuts at:      0    2157    2571 
   Size:       2157     414
 
BtgI C'CryG_G

Cuts at:      0    1861    2571 
   Size:       1861     710
 
DraI TTT'AAA

Cuts at:      0      81     251     560     980    1583    2315    2571 
   Size:         81     170     309     420     603     732     256

  Fragments arranged by size: 

                 732     603     420     309     256     170      81
 
EaeI y'GGCC_r

Cuts at:      0     309     401    1455    2402    2571 
   Size:        309      92    1054     947     169

  Fragments arranged by size: 

                1054     947     309     169      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     810    2571 
   Size:        810    1761
 
EcoICRI GAG'CTC

Cuts at:      0    2352    2571 
   Size:       2352     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1388    1520    2085    2438    2571 
   Size:       1087     301     132     565     353     133

  Fragments arranged by size: 

                1087     565     353     301     133     132
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2322    2571 
   Size:       2322     249
 
EcoRI G'AATT_C

Cuts at:      0     332     768    2457    2571 
   Size:        332     436    1689     114

  Fragments arranged by size: 

                1689     436     332     114
 
EcoRV GAT'ATC

Cuts at:      0    2339    2571 
   Size:       2339     232
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1437    2571 
   Size:       1437    1134
 
HaeII r_GCGC'y

Cuts at:      0     292    2571 
   Size:        292    2279
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1327    1359    2137    2169    2199    2231    2571 
   Size:       1327      32     778      32      30      32     340

  Fragments arranged by size: 

                1327     778     340      32      32      32      30
 
HincII GTy'rAC

Cuts at:      0     156     828    1445    2394    2571 
   Size:        156     672     617     949     177

  Fragments arranged by size: 

                 949     672     617     177     156
 
HindIII A'AGCT_T

Cuts at:      0     108    2571 
   Size:        108    2463
 
HpaI GTT'AAC

Cuts at:      0     156     828    2394    2571 
   Size:        156     672    1566     177

  Fragments arranged by size: 

                1566     672     177     156
 
MfeI C'AATT_G

Cuts at:      0      35     281    2571 
   Size:         35     246    2290

  Fragments arranged by size: 

                2290     246      35
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1782    2571 
   Size:       1782     789
 
MscI TGG'CCA

Cuts at:      0     311     403    1457    2404    2571 
   Size:        311      92    1054     947     167

  Fragments arranged by size: 

                1054     947     311     167      92
 
MslI CAynn'nnrTG

Cuts at:      0    1326    1795    1801    1919    2366    2507    2571 
   Size:       1326     469       6     118     447     141      64

  Fragments arranged by size: 

                1326     469     447     141     118      64       6
 
MspA1I CmG'CkG

Cuts at:      0     579    2571 
   Size:        579    1992
 
NcoI C'CATG_G

Cuts at:      0    1861    2571 
   Size:       1861     710
 
NsiI A_TGCA'T

Cuts at:      0    1552    2571 
   Size:       1552    1019
 
NspI r_CATG'y

Cuts at:      0     738    2236    2571 
   Size:        738    1498     335

  Fragments arranged by size: 

                1498     738     335
 
PmlI CAC'GTG

Cuts at:      0     385    2571 
   Size:        385    2186
 
PpuMI rG'GwC_Cy

Cuts at:      0    2322    2571 
   Size:       2322     249
 
PvuII CAG'CTG

Cuts at:      0     579    2571 
   Size:        579    1992
 
SacI G_AGCT'C

Cuts at:      0    2354    2571 
   Size:       2354     217
 
SanDI GG'GwC_CC

Cuts at:      0    2322    2571 
   Size:       2322     249
 
SfcI C'TryA_G

Cuts at:      0     134     506    2505    2571 
   Size:        134     372    1999      66

  Fragments arranged by size: 

                1999     372     134      66
 
SspI AAT'ATT

Cuts at:      0    1818    2571 
   Size:       1818     753
 
StyI C'CwwG_G

Cuts at:      0    1373    1861    2405    2571 
   Size:       1373     488     544     166

  Fragments arranged by size: 

                1373     544     488     166
 
TatI w'GTAC_w

Cuts at:      0    1787    2171    2571 
   Size:       1787     384     400

  Fragments arranged by size: 

                1787     400     384
 
XbaI T'CTAG_A

Cuts at:      0     904    2571 
   Size:        904    1667
 
XmnI GAAnn'nnTTC

Cuts at:      0     290     957    1572    2571 
   Size:        290     667     615     999

  Fragments arranged by size: 

                 999     667     615     290
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AlfI     ApoI     AseI     AvaI
    BanII     BbsI    BfrBI    BglII     BmrI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaWI    BseRI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI   BstEII    BstXI    BstYI   Bsu36I     BtgI     DraI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NsiI     NspI     PmlI    PpuMI    PvuII     SacI
    SanDI     SfcI     SspI     StyI     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI
     FseI     FspI    FspAI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI     ZraI
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