Restriction map for pF1KB7967
 (Linear) (Six-base) MAPSORT of: KIBB7967.seq  Check: 4662  from: 1  to: 849



KIBB7967.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AclI AA'CG_TT

Cuts at:      0     120     849 
   Size:        120     729
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     849 
   Size:        256     593
 
AgeI A'CCGG_T

Cuts at:      0     599     849 
   Size:        599     250
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     110     142     849 
   Size:        110      32     707

  Fragments arranged by size: 

                 707     110      32
 
ApaLI G'TGCA_C

Cuts at:      0      46     595     849 
   Size:         46     549     254

  Fragments arranged by size: 

                 549     254      46
 
ApoI r'AATT_y

Cuts at:      0     166     849 
   Size:        166     683
 
AvaI C'yCGr_G

Cuts at:      0     209     276     408     496     849 
   Size:        209      67     132      88     353

  Fragments arranged by size: 

                 353     209     132      88      67
 
BanI G'GyrC_C

Cuts at:      0     602     842     849 
   Size:        602     240       7

  Fragments arranged by size: 

                 602     240       7
 
BanII G_rGCy'C

Cuts at:      0      38     140     424     849 
   Size:         38     102     284     425

  Fragments arranged by size: 

                 425     284     102      38
 
BglII A'GATC_T

Cuts at:      0     183     849 
   Size:        183     666
 
Bme1580I G_kGCm'C

Cuts at:      0      50     497     599     849 
   Size:         50     447     102     250

  Fragments arranged by size: 

                 447     250     102      50
 
BmgBI CAC'GTC

Cuts at:      0       6     849 
   Size:          6     843
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     525     849 
   Size:        525     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     103     112     239     849 
   Size:         22      81       9     127     610

  Fragments arranged by size: 

                 610     127      81      22       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      46     292     849 
   Size:         46     246     557

  Fragments arranged by size: 

                 557     246      46
 
BsaWI w'CCGG_w

Cuts at:      0     453     599     849 
   Size:        453     146     250

  Fragments arranged by size: 

                 453     250     146
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     110     140     849 
   Size:        110      30     709

  Fragments arranged by size: 

                 709     110      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     414     603     849 
   Size:        100     314     189     246

  Fragments arranged by size: 

                 314     246     189     100
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     814     849 
   Size:         39     775      35

  Fragments arranged by size: 

                 775      39      35
 
BsiHKAI G_wGCw'C

Cuts at:      0      11      38      50     424     599     849 
   Size:         11      27      12     374     175     250

  Fragments arranged by size: 

                 374     250     175      27      12      11
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      83     849 
   Size:         83     766
 
Bsp1286I G_dGCh'C

Cuts at:      0      11      38      50     140     424     497     599     849 
   Size:         11      27      12      90     284      73     102     250

  Fragments arranged by size: 

                 284     250     102      90      73      27      12      11
            
 
BspEI T'CCGG_A

Cuts at:      0     453     849 
   Size:        453     396
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     112     849 
   Size:        112     737
 
BsrFI r'CCGG_y

Cuts at:      0      55     599     849 
   Size:         55     544     250

  Fragments arranged by size: 

                 544     250      55
 
BssSI C'ACGA_G

Cuts at:      0      49     849 
   Size:         49     800
 
BstYI r'GATC_y

Cuts at:      0     183     849 
   Size:        183     666
 
BtgI C'CryG_G

Cuts at:      0     362     849 
   Size:        362     487
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     581     849 
   Size:        581     268
 
DraI TTT'AAA

Cuts at:      0     780     849 
   Size:        780      69
 
EaeI y'GGCC_r

Cuts at:      0     365     849 
   Size:        365     484
 
EarI CTCTTCn'nnn_

Cuts at:      0     832     849 
   Size:        832      17
 
EcoICRI GAG'CTC

Cuts at:      0      36     422     849 
   Size:         36     386     427

  Fragments arranged by size: 

                 427     386      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     103     112     239     256     849 
   Size:         22      81       9     127      17     593

  Fragments arranged by size: 

                 593     127      81      22      17       9
 
EcoRV GAT'ATC

Cuts at:      0     242     849 
   Size:        242     607
 
FspI TGC'GCA

Cuts at:      0     526     849 
   Size:        526     323
 
HaeII r_GCGC'y

Cuts at:      0      73     849 
   Size:         73     776
 
HincII GTy'rAC

Cuts at:      0     740     849 
   Size:        740     109
 
HpaI GTT'AAC

Cuts at:      0     740     849 
   Size:        740     109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     849 
   Size:        404     445
 
MspA1I CmG'CkG

Cuts at:      0     530     663     849 
   Size:        530     133     186

  Fragments arranged by size: 

                 530     186     133
 
PfoI T'CCnGG_A

Cuts at:      0      29     849 
   Size:         29     820
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     849 
   Size:        110      32     707

  Fragments arranged by size: 

                 707     110      32
 
PstI C_TGCA'G

Cuts at:      0     622     849 
   Size:        622     227
 
SacI G_AGCT'C

Cuts at:      0      38     424     849 
   Size:         38     386     425

  Fragments arranged by size: 

                 425     386      38
 
ScaI AGT'ACT

Cuts at:      0      91     849 
   Size:         91     758
 
SfcI C'TryA_G

Cuts at:      0     618     849 
   Size:        618     231
 
SgrAI Cr'CCGG_yG

Cuts at:      0     599     849 
   Size:        599     250
 
SmaI CCC'GGG

Cuts at:      0     498     849 
   Size:        498     351
 
TatI w'GTAC_w

Cuts at:      0      89     849 
   Size:         89     760
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     172     204     534     566     849 
   Size:        172      32     330      32     283

  Fragments arranged by size: 

                 330     283     172      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201     849 
   Size:        201     648
 
XmaI C'CCGG_G

Cuts at:      0     496     849 
   Size:        496     353
 

 Enzymes that do cut:

     AclI     AcuI     AgeI     AloI    ApaLI     ApoI     AvaI     BanI
    BanII    BglII Bme1580I    BmgBI     BplI     BpmI     BsaI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrFI    BssSI    BstYI     BtgI    BtgZI     DraI     EaeI     EarI
  EcoICRI  Eco57MI    EcoRV     FspI    HaeII   HincII     HpaI     MmeI
   MspA1I     PfoI     PpiI     PstI     SacI     ScaI     SfcI    SgrAI
     SmaI     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AhdI
     AleI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
    EcoNI EcoO109I    EcoRI     FalI     FseI    FspAI    Hin4I  HindIII
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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