Restriction map for pF1KB6833
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        Eam1105I    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MseI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp45I      Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcyI          343  GACGCC               G[GA]^CG[CT]C
AlwNI         286  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       484  AGCGCT               AGC^GCT
Asp700I       141  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         302  TGCGCA               TGC^GCA
BalI          272  TGGCCA               TGG^CCA
BanI          355  GGCACC               G^G[CT][GA]CC
BanII         463  GAGCTC               G[GA]GC[CT]^C
BsaHI         343  GACGCC               G[GA]^CG[CT]C
BsrI          494  ACTGG                ACTGG(1/-1)
BstF5I        360  CATCC                GGATG(2/0)
Cfr13I        262  GGGCC                G^G[ACGT]CC
EarI           34  CTCTTC               CTCTTC(1/4)
Eco47III      484  AGCGCT               AGC^GCT
EcoT14I       275  CCATGG               C^C[AT][AT]GG
FokI          360  CATCC                GGATG(9/13)
FspI          302  TGCGCA               TGC^GCA
HgaI          343  GACGC                GACGC(5/10)
Hin1I         343  GACGCC               G[GA]^CG[CT]C
Ksp632I        34  CTCTTC               CTCTTC(1/4)
MaeII         296  ACGT                 A^CGT
MluNI         272  TGGCCA               TGG^CCA
MscI          272  TGGCCA               TGG^CCA
NcoI          275  CCATGG               C^CATGG
NlaIII        276  CATG                 CATG^
PflMI         275  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          412  GACTC                GAGTC(4/5)
SacI          463  GAGCTC               GAGCT^C
Sau96I        262  GGGCC                G^G[ACGT]CC
SfaNI         377  GCATC                GCATC(5/9)
SgrAI          51  CACCGGCG             C[GA]^CCGG[CT]G
StuI          176  AGGCCT               AGG^CCT
StyI          275  CCATGG               C^C[AT][AT]GG
TfiI          399  GATTC                G^A[AT]TC
TspRI         493  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        275  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          141  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         199  GAGCAC               G[AT]GC[AT]^C
              463  GAGCTC              
BseRI         466  CTCCTC               GAGGAG(10/8)
              184  GAGGAG              
BsiEI         234  CGGCCG               CG[GA][CT]^CG
              338  CGGCCG              
BsiHKAI       199  GAGCAC               G[AT]GC[AT]^C
              463  GAGCTC              
BsrFI          52  ACCGGC               [GA]^CCGG[CT]
              264  GCCGGT              
BstNI           9  CCTGG                CC^[AT]GG
               75  CCTGG               
Cfr10I         52  ACCGGC               [GA]^CCGG[CT]
              264  GCCGGT              
DpnII           3  GATC                 ^GATC
              438  GATC                
EagI          234  CGGCCG               C^GGCCG
              338  CGGCCG              
EclXI         234  CGGCCG               C^GGCCG
              338  CGGCCG              
Eco52I        234  CGGCCG               C^GGCCG
              338  CGGCCG              
EcoRII          9  CCTGG                ^CC[AT]GG
               75  CCTGG               
HinfI         399  GATTC                G^A[ACGT]TC
              412  GACTC               
KspI           41  CCGCGG               CCGC^GG
              363  CCGCGG              
MboI            3  GATC                 ^GATC
              438  GATC                
MboII          23  TCTTC                GAAGA(8/7)
               35  TCTTC               
MvaI            9  CCTGG                CC^[AT]GG
               75  CCTGG               
NdeII           3  GATC                 ^GATC
              438  GATC                
SacII          41  CCGCGG               CCGC^GG
              363  CCGCGG              
Sau3AI          3  GATC                 ^GATC
              438  GATC                
TaqI          134  TCGA                 T^CGA
              380  TCGA                
TthHB8I       134  TCGA                 T^CGA
              380  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BmyI        BsaJI       BsgI        BsiYI       BslI        
Bsp1286I    BssKI       BstUI       Cac8I       CfoI        
DsaI        EaeI        FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspA1I      MspI        MvnI        
MwoI        NciI        NlaIV       ScrFI       TseI        
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