Restriction map for pF1KE0162
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BalI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         355  CGCG                 CG^CG
AcsI          817  GAATTT               [GA]^AATT[CT]
AhdI          650  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          817  GAATTT               [GA]^AATT[CT]
Asp700I       846  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         650  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          287  CCCGGG               C^[CT]CG[GA]G
BamHI         681  GGATCC               G^GATCC
BfaI          736  CTAG                 C^TAG
BsoBI         287  CCCGGG               C^[CT]CG[GA]G
BspDI         424  ATCGAT               AT^CGAT
BspHI         909  TCATGA               T^CATGA
BstBI         128  TTCGAA               TT^CGAA
BstF5I        594  CATCC                GGATG(2/0)
BstUI         355  CGCG                 CG^CG
CfoI          356  GCGC                 GCG^C
ClaI          424  ATCGAT               AT^CGAT
DraI          484  TTTAAA               TTT^AAA
DsaI          471  CCATGG               C^C[GA][CT]GG
EaeI          103  CGGCCA               [CT]^GGCC[GA]
Eam1105I      650  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       471  CCATGG               C^C[AT][AT]GG
FokI          594  CATCC                GGATG(9/13)
HgaI           67  GCGTC                GACGC(5/10)
HhaI          356  GCGC                 GCG^C
HinP1I        356  GCGC                 G^CGC
HincII        703  GTTGAC               GT[CT]^[GA]AC
HindII        703  GTTGAC               GT[CT]^[GA]AC
HphI          413  TCACC                GGTGA(8/7)
MaeI          736  CTAG                 C^TAG
MslI          910  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          355  CGCG                 CG^CG
NaeI          395  GCCGGC               GCC^GGC
NcoI          471  CCATGG               C^CATGG
NgoMIV        395  GCCGGC               G^CCGGC
NspV          128  TTCGAA               TT^CGAA
RcaI          909  TCATGA               T^CATGA
SacI          712  GAGCTC               GAGCT^C
SfuI          128  TTCGAA               TT^CGAA
SmaI          287  CCCGGG               CCC^GGG
SmlI          581  CTCAAG               C^T[CT][GA]AG
StyI          471  CCATGG               C^C[AT][AT]GG
TfiI          851  GATTC                G^A[AT]TC
XmaCI         287  CCCGGG               C^CCGGG
XmaI          287  CCCGGG               C^CCGGG
XmnI          846  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AfaI          497  GTAC                 GT^AC
              762  GTAC                
AspHI         563  GTGCTC               G[AT]GC[AT]^C
              712  GAGCTC              
AvaII         249  GGACC                G^G[AT]CC
              871  GGACC               
BanI          350  GGCACC               G^G[CT][GA]CC
              448  GGCACC              
BanII         337  GAGCCC               G[GA]GC[CT]^C
              712  GAGCTC              
BsaI          373  GAGACC               GGTCTC(1/5)
              829  GAGACC              
BseRI         212  GAGGAG               GAGGAG(10/8)
              697  GAGGAG              
BsgI          433  CTGCAC               GTGCAG(16/14)
              185  GTGCAG              
BsiHKAI       563  GTGCTC               G[AT]GC[AT]^C
              712  GAGCTC              
BsmAI         373  GAGAC                GTCTC(1/5)
              829  GAGAC               
BsrFI         311  ACCGGC               [GA]^CCGG[CT]
              395  GCCGGC              
BsrI          598  CCAGT                ACTGG(1/-1)
              808  CCAGT               
BstYI         467  AGATCC               [GA]^GATC[CT]
              681  GGATCC              
Cfr10I        311  ACCGGC               [GA]^CCGG[CT]
              395  GCCGGC              
DdeI          168  CTCAG                C^T[ACGT]AG
              518  CTGAG               
DpnII         468  GATC                 ^GATC
              682  GATC                
DraII         248  AGGACCT              [GA]G^G[ACGT]CC[CT]
              870  GGGACCC             
EarI          887  CTCTTC               CTCTTC(1/4)
              622  GAAGAG              
EcoO109I      248  AGGACCT              [GA]G^G[ACGT]CC[CT]
              870  GGGACCC             
FauI          315  GCGGG                CCCGC(4/6)
              635  GCGGG               
Ksp632I       887  CTCTTC               CTCTTC(1/4)
              622  GAAGAG              
MboI          468  GATC                 ^GATC
              682  GATC                
MboII         622  GAAGA                GAAGA(8/7)
              888  TCTTC               
MflI          467  AGATCC               [GA]^GATC[CT]
              681  GGATCC              
MseI          485  TTAA                 T^TAA
              821  TTAA                
NdeII         468  GATC                 ^GATC
              682  GATC                
PflMI         246  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              568  CCAGAACCTGG         
PpuMI         248  AGGACCT              [GA]G^G[AT]CC[CT]
              870  GGGACCC             
RsaI          497  GTAC                 GT^AC
              762  GTAC                
Sau3AI        468  GATC                 ^GATC
              682  GATC                
SfaNI         586  GATGC                GCATC(5/9)
              593  GCATC               
Tru9I         485  TTAA                 T^TAA
              821  TTAA                
TspRI         721  CACTG                CA[GC]TG[ACGT][ACGT]^
              809  CAGTG               
Van91I        246  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              568  CCAGAACCTGG         
XhoII         467  AGATCC               [GA]^GATC[CT]
              681  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      EcoRII      Fnu4HI      HaeIII      
HapII       HinfI       HpaII       ItaI        MnlI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       TaqI        
TseI        Tsp509I     TthHB8I     XcmI        
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