Restriction map for pF1KE0162
 (Linear) (Six-base) MAPSORT of: KIEE0162.seq  Check: 3634  from: 1  to: 930



KIEE0162.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     192     914     930 
   Size:         76     116     722      16

  Fragments arranged by size: 

                 722     116      76      16
 
AhdI GACnn_n'nnGTC

Cuts at:      0     655     930 
   Size:        655     275
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     873     905     930 
   Size:        873      32      25

  Fragments arranged by size: 

                 873      32      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     175     252     574     814     930 
   Size:        175      77     322     240     116

  Fragments arranged by size: 

                 322     240     175     116      77
 
ApoI r'AATT_y

Cuts at:      0     817     930 
   Size:        817     113
 
AvaI C'yCGr_G

Cuts at:      0     287     930 
   Size:        287     643
 
BamHI G'GATC_C

Cuts at:      0     681     930 
   Size:        681     249
 
BanI G'GyrC_C

Cuts at:      0     350     448     930 
   Size:        350      98     482

  Fragments arranged by size: 

                 482     350      98
 
BanII G_rGCy'C

Cuts at:      0     341     716     930 
   Size:        341     375     214

  Fragments arranged by size: 

                 375     341     214
 
Bme1580I G_kGCm'C

Cuts at:      0     353     748     930 
   Size:        353     395     182

  Fragments arranged by size: 

                 395     353     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     592     930 
   Size:        592     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     239     484     551     715     930 
   Size:         47     192     245      67     164     215

  Fragments arranged by size: 

                 245     215     192     164      67      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     930 
   Size:        566     364
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     367     823     930 
   Size:        367     456     107

  Fragments arranged by size: 

                 456     367     107
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     164     194     930 
   Size:        164      30     736

  Fragments arranged by size: 

                 736     164      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     227     712     930 
   Size:        227     485     218

  Fragments arranged by size: 

                 485     227     218
 
BseYI C'CCAG_C

Cuts at:      0      38     381     765     837     930 
   Size:         38     343     384      72      93

  Fragments arranged by size: 

                 384     343      93      72      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     418     930 
   Size:        206     212     512

  Fragments arranged by size: 

                 512     212     206
 
BsiHKAI G_wGCw'C

Cuts at:      0     567     716     930 
   Size:        567     149     214

  Fragments arranged by size: 

                 567     214     149
 
Bsp1286I G_dGCh'C

Cuts at:      0     341     353     567     716     748     930 
   Size:        341      12     214     149      32     182

  Fragments arranged by size: 

                 341     214     182     149      32      12
 
BspHI T'CATG_A

Cuts at:      0     909     930 
   Size:        909      21
 
BsrFI r'CCGG_y

Cuts at:      0     311     395     930 
   Size:        311      84     535

  Fragments arranged by size: 

                 535     311      84
 
BstBI TT'CG_AA

Cuts at:      0     129     930 
   Size:        129     801
 
BstYI r'GATC_y

Cuts at:      0     467     681     930 
   Size:        467     214     249

  Fragments arranged by size: 

                 467     249     214
 
BtgI C'CryG_G

Cuts at:      0     471     930 
   Size:        471     459
 
ClaI AT'CG_AT

Cuts at:      0     425     930 
   Size:        425     505
 
DraI TTT'AAA

Cuts at:      0     486     930 
   Size:        486     444
 
EaeI y'GGCC_r

Cuts at:      0     103     930 
   Size:        103     827
 
EarI CTCTTCn'nnn_

Cuts at:      0     617     893     930 
   Size:        617     276      37

  Fragments arranged by size: 

                 617     276      37
 
EcoICRI GAG'CTC

Cuts at:      0     714     930 
   Size:        714     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47      76     192     239     484     551     715     914 
   Size:         47      29     116      47     245      67     164     199

Cuts at:    914     930 
   Size:         16

  Fragments arranged by size: 

                 245     199     164     116      67      47      47      29
                  16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     871     930 
   Size:        249     622      59

  Fragments arranged by size: 

                 622     249      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     392     424     641     673     930 
   Size:        392      32     217      32     257

  Fragments arranged by size: 

                 392     257     217      32      32
 
HincII GTy'rAC

Cuts at:      0     705     930 
   Size:        705     225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     930 
   Size:        226     704
 
MslI CAynn'nnrTG

Cuts at:      0     914     930 
   Size:        914      16
 
NaeI GCC'GGC

Cuts at:      0     397     930 
   Size:        397     533
 
NcoI C'CATG_G

Cuts at:      0     471     930 
   Size:        471     459
 
NgoMIV G'CCGG_C

Cuts at:      0     395     930 
   Size:        395     535
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252     574     930 
   Size:        252     322     356

  Fragments arranged by size: 

                 356     322     252
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     871     930 
   Size:        249     622      59

  Fragments arranged by size: 

                 622     249      59
 
SacI G_AGCT'C

Cuts at:      0     716     930 
   Size:        716     214
 
SanDI GG'GwC_CC

Cuts at:      0     871     930 
   Size:        871      59
 
SmaI CCC'GGG

Cuts at:      0     289     930 
   Size:        289     641
 
SmlI C'TyrA_G

Cuts at:      0     581     930 
   Size:        581     349
 
StyI C'CwwG_G

Cuts at:      0     471     930 
   Size:        471     459
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     430     930 
   Size:        430     500
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     268     300     930 
   Size:        268      32     630

  Fragments arranged by size: 

                 630     268      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      46     459     874     930 
   Size:         46     413     415      56

  Fragments arranged by size: 

                 415     413      56      46
 
XmaI C'CCGG_G

Cuts at:      0     287     930 
   Size:        287     643
 
XmnI GAAnn'nnTTC

Cuts at:      0     850     930 
   Size:        850      80
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApoI     AvaI    BamHI     BanI
    BanII Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrFI    BstBI    BstYI
     BtgI     ClaI     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    Hin4I   HincII     MmeI     MslI     NaeI     NcoI   NgoMIV    PflMI
    PpuMI     SacI    SanDI     SmaI     SmlI     StyI    TaqII     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI
     ZraI
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