Restriction map for pF1KE0116
 (Linear) (Six-base) MAPSORT of: KIEE0116.seq  Check: 6196  from: 1  to: 1170



KIEE0116.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

Acc65I G'GTAC_C

Cuts at:      0     608    1170 
   Size:        608     562
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     316     532     628    1053    1170 
   Size:        176     140     216      96     425     117

  Fragments arranged by size: 

                 425     216     176     140     117      96
 
AlwNI CAG_nnn'CTG

Cuts at:      0     332    1170 
   Size:        332     838
 
ApaLI G'TGCA_C

Cuts at:      0      37    1170 
   Size:         37    1133
 
ApoI r'AATT_y

Cuts at:      0     930    1008    1170 
   Size:        930      78     162

  Fragments arranged by size: 

                 930     162      78
 
AvaI C'yCGr_G

Cuts at:      0    1082    1101    1170 
   Size:       1082      19      69

  Fragments arranged by size: 

                1082      69      19
 
AvrII C'CTAG_G

Cuts at:      0     381    1170 
   Size:        381     789
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0       6      39    1170 
   Size:          6      33    1131

  Fragments arranged by size: 

                1131      33       6
 
BanI G'GyrC_C

Cuts at:      0     608    1170 
   Size:        608     562
 
BanII G_rGCy'C

Cuts at:      0     977    1170 
   Size:        977     193
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     151     526    1170 
   Size:        151     375     644

  Fragments arranged by size: 

                 644     375     151
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1125    1159    1170 
   Size:       1125      34      11

  Fragments arranged by size: 

                1125      34      11
 
BfrBI ATG'CAT

Cuts at:      0    1020    1170 
   Size:       1020     150
 
BglI GCCn_nnn'nGGC

Cuts at:      0     133     739    1170 
   Size:        133     606     431

  Fragments arranged by size: 

                 606     431     133
 
Bme1580I G_kGCm'C

Cuts at:      0      41    1170 
   Size:         41    1129
 
BmgBI CAC'GTC

Cuts at:      0      36    1170 
   Size:         36    1134
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     409    1170 
   Size:        409     761
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     278     910    1018    1170 
   Size:         73     205     632     108     152

  Fragments arranged by size: 

                 632     205     152     108      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     868     961    1170 
   Size:        534     334      93     209

  Fragments arranged by size: 

                 534     334     209      93
 
BsaHI Gr'CG_yC

Cuts at:      0     959    1170 
   Size:        959     211
 
BsaWI w'CCGG_w

Cuts at:      0    1025    1170 
   Size:       1025     145
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359    1170 
   Size:        329      30     811

  Fragments arranged by size: 

                 811     329      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     667    1170 
   Size:        238     429     503

  Fragments arranged by size: 

                 503     429     238
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1170 
   Size:        547     623
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     977    1170 
   Size:         41     936     193

  Fragments arranged by size: 

                 936     193      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     712    1170 
   Size:        712     458
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     977    1170 
   Size:         41     936     193

  Fragments arranged by size: 

                 936     193      41
 
BsrFI r'CCGG_y

Cuts at:      0     605    1170 
   Size:        605     565
 
BsrGI T'GTAC_A

Cuts at:      0     461    1170 
   Size:        461     709
 
BssSI C'ACGA_G

Cuts at:      0     274    1170 
   Size:        274     896
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     534    1170 
   Size:        534     636
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175    1170 
   Size:        175     995
 
BstYI r'GATC_y

Cuts at:      0     860    1170 
   Size:        860     310
 
Bsu36I CC'TnA_GG

Cuts at:      0     476    1170 
   Size:        476     694
 
BtgI C'CryG_G

Cuts at:      0     155    1170 
   Size:        155    1015
 
EaeI y'GGCC_r

Cuts at:      0     539     842    1170 
   Size:        539     303     328

  Fragments arranged by size: 

                 539     328     303
 
EarI CTCTTCn'nnn_

Cuts at:      0     292    1170 
   Size:        292     878
 
EcoICRI GAG'CTC

Cuts at:      0     975    1170 
   Size:        975     195
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     176     278     316     532     628     910    1018 
   Size:         73     103     102      38     216      96     282     108

Cuts at:   1018    1053    1170 
   Size:         35     117

  Fragments arranged by size: 

                 282     216     117     108     103     102      96      73
                  38      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     832    1170 
   Size:        832     338
 
EcoRI G'AATT_C

Cuts at:      0     930    1170 
   Size:        930     240
 
EcoRV GAT'ATC

Cuts at:      0    1118    1170 
   Size:       1118      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     258    1170 
   Size:        258     912
 
HaeII r_GCGC'y

Cuts at:      0     705    1170 
   Size:        705     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     711     743    1170 
   Size:        711      32     427

  Fragments arranged by size: 

                 711     427      32
 
HindIII A'AGCT_T

Cuts at:      0     367     544    1170 
   Size:        367     177     626

  Fragments arranged by size: 

                 626     367     177
 
KpnI G_GTAC'C

Cuts at:      0     612    1170 
   Size:        612     558
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     666    1170 
   Size:        666     504
 
MscI TGG'CCA

Cuts at:      0     541    1170 
   Size:        541     629
 
MslI CAynn'nnrTG

Cuts at:      0     235     872    1156    1170 
   Size:        235     637     284      14

  Fragments arranged by size: 

                 637     284     235      14
 
MspA1I CmG'CkG

Cuts at:      0     175     294     897    1170 
   Size:        175     119     603     273

  Fragments arranged by size: 

                 603     273     175     119
 
NcoI C'CATG_G

Cuts at:      0     155    1170 
   Size:        155    1015
 
NsiI A_TGCA'T

Cuts at:      0    1022    1170 
   Size:       1022     148
 
NspI r_CATG'y

Cuts at:      0     882    1170 
   Size:        882     288
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     870    1170 
   Size:        870     300
 
PfoI T'CCnGG_A

Cuts at:      0     317     347     968    1170 
   Size:        317      30     621     202

  Fragments arranged by size: 

                 621     317     202      30
 
PstI C_TGCA'G

Cuts at:      0     698    1170 
   Size:        698     472
 
PvuII CAG'CTG

Cuts at:      0     175     294    1170 
   Size:        175     119     876

  Fragments arranged by size: 

                 876     175     119
 
SacI G_AGCT'C

Cuts at:      0     977    1170 
   Size:        977     193
 
SfcI C'TryA_G

Cuts at:      0     337     694    1170 
   Size:        337     357     476

  Fragments arranged by size: 

                 476     357     337
 
SmaI CCC'GGG

Cuts at:      0    1084    1103    1170 
   Size:       1084      19      67

  Fragments arranged by size: 

                1084      67      19
 
SmlI C'TyrA_G

Cuts at:      0     513     883     976    1170 
   Size:        513     370      93     194

  Fragments arranged by size: 

                 513     370     194      93
 
SrfI GCCC'GGGC

Cuts at:      0    1084    1170 
   Size:       1084      86
 
SspI AAT'ATT

Cuts at:      0     285    1170 
   Size:        285     885
 
StuI AGG'CCT

Cuts at:      0     733    1170 
   Size:        733     437
 
StyI C'CwwG_G

Cuts at:      0     155     381    1170 
   Size:        155     226     789

  Fragments arranged by size: 

                 789     226     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      56    1090    1170 
   Size:         56    1034      80

  Fragments arranged by size: 

                1034      80      56
 
TatI w'GTAC_w

Cuts at:      0     239     311     461    1170 
   Size:        239      72     150     709

  Fragments arranged by size: 

                 709     239     150      72
 
XmaI C'CCGG_G

Cuts at:      0    1082    1101    1170 
   Size:       1082      19      69

  Fragments arranged by size: 

                1082      69      19
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI    ApaLI     ApoI     AvaI    AvrII     BaeI
     BanI    BanII     BbsI     BcgI    BfrBI     BglI Bme1580I    BmgBI
     BplI     BpmI    BpuEI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BsrFI    BsrGI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KpnI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI    PflMI     PfoI
     PstI    PvuII     SacI     SfcI     SmaI     SmlI     SrfI     SspI
     StuI     StyI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
    BamHI     BbeI    BbvCI    BciVI     BclI    BglII     BlpI     BmrI
     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BseYI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BstBI
   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI EcoO109I     FseI     FspI    FspAI   HincII
     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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