Restriction map for pF1KE0120
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BamHI       
BanI        BbeI        BbsI        BcgI        BciVI       
BclI        BglI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tsp45I      Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          766  GACGCC               G[GA]^CG[CT]C
AfaI          609  GTAC                 GT^AC
AflII         811  CTTAAG               C^TTAAG
ApaI          160  GGGCCC               GGGCC^C
AvaI          505  CCCGAG               C^[CT]CG[GA]G
AvrII         186  CCTAGG               C^CTAGG
BalI            2  TGGCCA               TGG^CCA
BbrPI         727  CACGTG               CAC^GTG
BfaI          187  CTAG                 C^TAG
BfrI          811  CTTAAG               C^TTAAG
BglII         821  AGATCT               A^GATCT
BlnI          186  CCTAGG               C^CTAGG
BsaAI         727  CACGTG               [CT]AC^GT[GA]
BsaHI         766  GACGCC               G[GA]^CG[CT]C
BsmAI         787  GTCTC                GTCTC(1/5)
BsmBI         786  CGTCTC               CGTCTC(1/5)
BsmFI         389  GGGAC                GGGAC(10/14)
BsoBI         505  CCCGAG               C^[CT]CG[GA]G
BsrBI          31  GAGCGG               CCGCTC(-3/-3)
BsrFI         473  ACCGGC               [GA]^CCGG[CT]
BstAPI        618  GCATGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI           5  CCACCGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        473  ACCGGC               [GA]^CCGG[CT]
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoT14I       186  CCTAGG               C^C[AT][AT]GG
Hin1I         766  GACGCC               G[GA]^CG[CT]C
HincII        568  GTCAAC               GT[CT]^[GA]AC
HindII        568  GTCAAC               GT[CT]^[GA]AC
HinfI         225  GACTC                G^A[ACGT]TC
MaeI          187  CTAG                 C^TAG
MboII         267  GAAGA                GAAGA(8/7)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MseI          812  TTAA                 T^TAA
NspI          618  GCATGC               [GA]CATG^[CT]
PflMI         836  CCAGCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          225  GACTC                GAGTC(4/5)
PmaCI         727  CACGTG               CAC^GTG
PmlI          727  CACGTG               CAC^GTG
PpuMI         422  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          609  GTAC                 GT^AC
SexAI         206  ACCAGGT              A^CC[AT]GGT
SphI          618  GCATGC               GCATG^C
StuI          119  AGGCCT               AGG^CCT
StyI          186  CCTAGG               C^C[AT][AT]GG
TaqI          695  TCGA                 T^CGA
Tru9I         812  TTAA                 T^TAA
TthHB8I       695  TCGA                 T^CGA
Van91I        836  CCAGCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          574  GTCTAC               GT^[AC][GT]AC
              775  GTCTAC              
AccII         530  CGCG                 CG^CG
              666  CGCG                
Alw44I         79  GTGCAC               G^TGCAC
              730  GTGCAC              
AlwI          336  GATCC                GGATC(4/5)
              750  GATCC               
ApaLI          79  GTGCAC               G^TGCAC
              730  GTGCAC              
AspHI          79  GTGCAC               G[AT]GC[AT]^C
              730  GTGCAC              
AvaII         390  GGACC                G^G[AT]CC
              423  GGTCC               
BbvCI         243  CCTCAGC              CCTCAGC(-5/-2)
              418  GCTGAGG             
BseRI          46  GAGGAG               GAGGAG(10/8)
               49  GAGGAG              
BsgI           67  CTGCAC               GTGCAG(16/14)
              292  CTGCAC              
BsiHKAI        79  GTGCAC               G[AT]GC[AT]^C
              730  GTGCAC              
BspMI         374  ACCTGC               ACCTGC(4/8)
              560  ACCTGC              
BsrI          842  ACTGG                ACTGG(1/-1)
              402  CCAGT               
BstUI         530  CGCG                 CG^CG
              666  CGCG                
BstYI         335  AGATCC               [GA]^GATC[CT]
              821  AGATCT              
DraII         159  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              422  AGGTCCT             
DraIII         82  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              733  CACAAAGTG           
DrdI          559  GACCTGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              766  GACGCCCTGGTC        
EcoNI         111  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              243  CCTCAGCCAGG         
EcoO109I      159  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              422  AGGTCCT             
HaeII         415  GGCGCT               [GA]GCGC^[CT]
              710  AGCGCC              
HgaI          439  GACGC                GACGC(5/10)
              766  GACGC               
MaeII         572  ACGT                 A^CGT
              728  ACGT                
MflI          335  AGATCC               [GA]^GATC[CT]
              821  AGATCT              
MslI            6  CACCGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              528  CACGCGGATG          
MvnI          530  CGCG                 CG^CG
              666  CGCG                
XhoII         335  AGATCC               [GA]^GATC[CT]
              821  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AlwNI       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TseI        
TspRI       
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