Restriction map for pF1KE0120
 (Linear) (Six-base) MAPSORT of: KIEE0120.seq  Check: 5346  from: 1  to: 861



KIEE0120.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:      0     575     776     861 
   Size:        575     201      85

  Fragments arranged by size: 

                 575     201      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     861 
   Size:        286     575
 
AflII C'TTAA_G

Cuts at:      0     811     861 
   Size:        811      50
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     254     861 
   Size:        254     607
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69     861 
   Size:         37      32     792

  Fragments arranged by size: 

                 792      37      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     714     842     861 
   Size:        291     423     128      19

  Fragments arranged by size: 

                 423     291     128      19
 
ApaI G_GGCC'C

Cuts at:      0     164     861 
   Size:        164     697
 
ApaLI G'TGCA_C

Cuts at:      0      79     730     861 
   Size:         79     651     131

  Fragments arranged by size: 

                 651     131      79
 
AvaI C'yCGr_G

Cuts at:      0     505     861 
   Size:        505     356
 
AvrII C'CTAG_G

Cuts at:      0     186     861 
   Size:        186     675
 
BanII G_rGCy'C

Cuts at:      0     148     164     468     706     861 
   Size:        148      16     304     238     155

  Fragments arranged by size: 

                 304     238     155     148      16
 
BbvCI CC'TCA_GC

Cuts at:      0     244     419     861 
   Size:        244     175     442

  Fragments arranged by size: 

                 442     244     175
 
BglII A'GATC_T

Cuts at:      0     821     861 
   Size:        821      40
 
Bme1580I G_kGCm'C

Cuts at:      0      83     164     734     861 
   Size:         83      81     570     127

  Fragments arranged by size: 

                 570     127      83      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     720     861 
   Size:        720     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     736     819     861 
   Size:         64     672      83      42

  Fragments arranged by size: 

                 672      83      64      42
 
Bpu10I CC'TnA_GC

Cuts at:      0     244     419     861 
   Size:        244     175     442

  Fragments arranged by size: 

                 442     244     175
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     412     861 
   Size:         97     315     449

  Fragments arranged by size: 

                 449     315      97
 
BsaAI yAC'GTr

Cuts at:      0     729     861 
   Size:        729     132
 
BsaHI Gr'CG_yC

Cuts at:      0     767     861 
   Size:        767      94
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     808     838     861 
   Size:         37      30     741      30      23

  Fragments arranged by size: 

                 741      37      30      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61      64     861 
   Size:         61       3     797

  Fragments arranged by size: 

                 797      61       3
 
BseYI C'CCAG_C

Cuts at:      0     654     658     861 
   Size:        654       4     203

  Fragments arranged by size: 

                 654     203       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     277     861 
   Size:         52     225     584

  Fragments arranged by size: 

                 584     225      52
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     734     861 
   Size:         83     651     127

  Fragments arranged by size: 

                 651     127      83
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     792     861 
   Size:        792      69
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     148     164     468     706     734     861 
   Size:         83      65      16     304     238      28     127

  Fragments arranged by size: 

                 304     238     127      83      65      28      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     569     861 
   Size:        383     186     292

  Fragments arranged by size: 

                 383     292     186
 
BsrBI CCG'CTC

Cuts at:      0      33     861 
   Size:         33     828
 
BsrFI r'CCGG_y

Cuts at:      0     473     861 
   Size:        473     388
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     624     861 
   Size:        624     237
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     861 
   Size:         12     849
 
BstYI r'GATC_y

Cuts at:      0     335     821     861 
   Size:        335     486      40

  Fragments arranged by size: 

                 486     335      40
 
BtsI GCAGTG_nn'

Cuts at:      0     325     449     861 
   Size:        325     124     412

  Fragments arranged by size: 

                 412     325     124
 
DraIII CAC_nnn'GTG

Cuts at:      0      87     738     861 
   Size:         87     651     123

  Fragments arranged by size: 

                 651     123      87
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     565     772     861 
   Size:        565     207      89

  Fragments arranged by size: 

                 565     207      89
 
EaeI y'GGCC_r

Cuts at:      0       2     861 
   Size:          2     859
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      43     268     640     861 
   Size:         43     225     372     221

  Fragments arranged by size: 

                 372     225     221      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     286     736     819     861 
   Size:         64     222     450      83      42

  Fragments arranged by size: 

                 450     222      83      64      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     247     861 
   Size:        115     132     614

  Fragments arranged by size: 

                 614     132     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     160     423     861 
   Size:        160     263     438

  Fragments arranged by size: 

                 438     263     160
 
HaeII r_GCGC'y

Cuts at:      0     419     714     861 
   Size:        419     295     147

  Fragments arranged by size: 

                 419     295     147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     808     840     861 
   Size:        808      32      21

  Fragments arranged by size: 

                 808      32      21
 
HincII GTy'rAC

Cuts at:      0     570     861 
   Size:        570     291
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     861 
   Size:        581     280
 
MscI TGG'CCA

Cuts at:      0       4     861 
   Size:          4     857
 
MslI CAynn'nnrTG

Cuts at:      0      10     532     861 
   Size:         10     522     329

  Fragments arranged by size: 

                 522     329      10
 
MspA1I CmG'CkG

Cuts at:      0      59      99     194     288     456     521     615     658 
   Size:         59      40      95      94     168      65      94      43

Cuts at:    658     861 
   Size:        203

  Fragments arranged by size: 

                 203     168      95      94      94      65      59      43
                  40
 
NspI r_CATG'y

Cuts at:      0     622     861 
   Size:        622     239
 
PasI CC'CwG_GG

Cuts at:      0     682     861 
   Size:        682     179
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     842     861 
   Size:        842      19
 
PfoI T'CCnGG_A

Cuts at:      0     305     338     861 
   Size:        305      33     523

  Fragments arranged by size: 

                 523     305      33
 
PmlI CAC'GTG

Cuts at:      0     729     861 
   Size:        729     132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      37      69     861 
   Size:         37      32     792

  Fragments arranged by size: 

                 792      37      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     423     861 
   Size:        423     438
 
PspOMI G'GGCC_C

Cuts at:      0     160     861 
   Size:        160     701
 
PstI C_TGCA'G

Cuts at:      0     104     233     332     455     629     797     861 
   Size:        104     129      99     123     174     168      64

  Fragments arranged by size: 

                 174     168     129     123     104      99      64
 
PvuII CAG'CTG

Cuts at:      0      99     194     288     456     615     658     861 
   Size:         99      95      94     168     159      43     203

  Fragments arranged by size: 

                 203     168     159      99      95      94      43
 
SexAI A'CCwGG_T

Cuts at:      0     206     861 
   Size:        206     655
 
SfcI C'TryA_G

Cuts at:      0     100     229     328     451     625     793     849     861 
   Size:        100     129      99     123     174     168      56      12

  Fragments arranged by size: 

                 174     168     129     123     100      99      56      12
            
 
SmlI C'TyrA_G

Cuts at:      0     112     427     811     861 
   Size:        112     315     384      50

  Fragments arranged by size: 

                 384     315     112      50
 
SphI G_CATG'C

Cuts at:      0     622     861 
   Size:        622     239
 
StuI AGG'CCT

Cuts at:      0     121     861 
   Size:        121     740
 
StyI C'CwwG_G

Cuts at:      0     186     861 
   Size:        186     675
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     304     336     861 
   Size:        304      32     525

  Fragments arranged by size: 

                 525     304      32
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AlfI     AloI    AlwNI     ApaI    ApaLI
     AvaI    AvrII    BanII    BbvCI    BglII Bme1580I     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI
    BstYI     BtsI   DraIII     DrdI     EaeI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI
     PstI    PvuII    SexAI     SfcI     SmlI     SphI     StuI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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