Restriction map for pF1KE0410
 (Linear) (Six-base) MAPSORT of: KIEE0410.seq  Check: 5075  from: 1  to: 555



KIEE0410.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     495     555 
   Size:        495      60
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74      92     555 
   Size:         74      18     463

  Fragments arranged by size: 

                 463      74      18
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     187     555 
   Size:        187     368
 
AlwNI CAG_nnn'CTG

Cuts at:      0     199     427     430     555 
   Size:        199     228       3     125

  Fragments arranged by size: 

                 228     199     125       3
 
AvaI C'yCGr_G

Cuts at:      0     439     449     555 
   Size:        439      10     106

  Fragments arranged by size: 

                 439     106      10
 
BanI G'GyrC_C

Cuts at:      0      42     304     481     555 
   Size:         42     262     177      74

  Fragments arranged by size: 

                 262     177      74      42
 
BanII G_rGCy'C

Cuts at:      0     188     555 
   Size:        188     367
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     522     529     555 
   Size:        522       7      26

  Fragments arranged by size: 

                 522      26       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     153     555 
   Size:        153     402
 
BfrBI ATG'CAT

Cuts at:      0      92     367     555 
   Size:         92     275     188

  Fragments arranged by size: 

                 275     188      92
 
BlpI GC'TnA_GC

Cuts at:      0     550     555 
   Size:        550       5
 
Bme1580I G_kGCm'C

Cuts at:      0      47     125     309     486     555 
   Size:         47      78     184     177      69

  Fragments arranged by size: 

                 184     177      78      69      47
 
BmgBI CAC'GTC

Cuts at:      0     210     555 
   Size:        210     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     555 
   Size:        487      68
 
BsaAI yAC'GTr

Cuts at:      0     177     555 
   Size:        177     378
 
BsaWI w'CCGG_w

Cuts at:      0      80     555 
   Size:         80     475
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     262     406     409     555 
   Size:        262     144       3     146

  Fragments arranged by size: 

                 262     146     144       3
 
BseYI C'CCAG_C

Cuts at:      0      48     485     555 
   Size:         48     437      70

  Fragments arranged by size: 

                 437      70      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     258     367     529     555 
   Size:        157     101     109     162      26

  Fragments arranged by size: 

                 162     157     109     101      26
 
BsiEI CG_ry'CG

Cuts at:      0     537     555 
   Size:        537      18
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     125     188     309     486     555 
   Size:         47      78      63     121     177      69

  Fragments arranged by size: 

                 177     121      78      69      63      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     230     495     555 
   Size:        230     265      60

  Fragments arranged by size: 

                 265     230      60
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     427     430     546     555 
   Size:        427       3     116       9

  Fragments arranged by size: 

                 427     116       9       3
 
BtgI C'CryG_G

Cuts at:      0     296     555 
   Size:        296     259
 
DraIII CAC_nnn'GTG

Cuts at:      0     180     555 
   Size:        180     375
 
EaeI y'GGCC_r

Cuts at:      0     201     555 
   Size:        201     354
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74      92     487     555 
   Size:         74      18     395      68

  Fragments arranged by size: 

                 395      74      68      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     445     555 
   Size:        445     110
 
EcoRV GAT'ATC

Cuts at:      0      78     555 
   Size:         78     477
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     546     555 
   Size:        546       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     397     429     555 
   Size:        397      32     126

  Fragments arranged by size: 

                 397     126      32
 
MscI TGG'CCA

Cuts at:      0     203     555 
   Size:        203     352
 
MslI CAynn'nnrTG

Cuts at:      0     193     555 
   Size:        193     362
 
MspA1I CmG'CkG

Cuts at:      0      52     427     492     519     555 
   Size:         52     375      65      27      36

  Fragments arranged by size: 

                 375      65      52      36      27
 
NsiI A_TGCA'T

Cuts at:      0      94     369     555 
   Size:         94     275     186

  Fragments arranged by size: 

                 275     186      94
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     199     210     555 
   Size:        199      11     345

  Fragments arranged by size: 

                 345     199      11
 
PfoI T'CCnGG_A

Cuts at:      0     464     555 
   Size:        464      91
 
PmlI CAC'GTG

Cuts at:      0     177     555 
   Size:        177     378
 
PpuMI rG'GwC_Cy

Cuts at:      0     445     555 
   Size:        445     110
 
PstI C_TGCA'G

Cuts at:      0     506     555 
   Size:        506      49
 
PvuI CG_AT'CG

Cuts at:      0     537     555 
   Size:        537      18
 
PvuII CAG'CTG

Cuts at:      0      52     427     555 
   Size:         52     375     128

  Fragments arranged by size: 

                 375     128      52
 
SanDI GG'GwC_CC

Cuts at:      0     445     555 
   Size:        445     110
 
SfcI C'TryA_G

Cuts at:      0     502     555 
   Size:        502      53
 
StuI AGG'CCT

Cuts at:      0     217     555 
   Size:        217     338
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     555 
   Size:        292     263
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     AvaI     BanI    BanII     BbsI
    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BpmI    BsaAI    BsaWI
    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspMI   BstAPI     BtgI
   DraIII     EaeI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I     MscI
     MslI   MspA1I     NsiI    PflMI     PfoI     PmlI    PpuMI     PstI
     PvuI    PvuII    SanDI     SfcI     StuI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI
     BglI    BglII     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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