Restriction map for pF1KB9865
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AvrII       BamHI       BbrPI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspEI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       CpoI        DraI        DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MaeI        MaeII       MfeI        MluI        MroI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SalI        SbfI        ScaI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcyI           79  GGCGCC               G[GA]^CG[CT]C
AflIII        209  ACATGT               A^C[GA][CT]GT
AhdI          181  GACGAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       194  AGCGCT               AGC^GCT
Asp700I      1089  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         181  GACGAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         653  TGCGCA               TGC^GCA
BanII         982  GAGCCC               G[GA]GC[CT]^C
BbeI           79  GGCGCC               GGCGC^C
BbsI          475  GTCTTC               GAAGAC(2/6)
BbvCI         343  GCTGAGG              CCTCAGC(-5/-2)
BcgI          128  GCAACCGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         475  GTCTTC               GAAGAC(2/6)
BsaBI         744  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          79  GGCGCC               G[GA]^CG[CT]C
BsaI         1178  GAGACC               GGTCTC(1/5)
BsgI           58  CTGCAC               GTGCAG(16/14)
BspDI         751  ATCGAT               AT^CGAT
BspHI         524  TCATGA               T^CATGA
BspLU11I      209  ACATGT               A^CATGT
BsrBI         835  CCGCTC               CCGCTC(-3/-3)
BstAPI        620  GCAAGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI          751  ATCGAT               AT^CGAT
Eam1105I      181  GACGAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      194  AGCGCT               AGC^GCT
FspI          653  TGCGCA               TGC^GCA
Hin1I          79  GGCGCC               G[GA]^CG[CT]C
KasI           79  GGCGCC               G^GCGCC
MamI          744  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI           79  GGCGCC               GG^CGCC
PflMI         663  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1280  GACGGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1065  CTGCAG               CTGCA^G
RcaI          524  TCATGA               T^CATGA
SexAI          62  ACCTGGT              A^CC[AT]GGT
SfiI            9  GGCCAGCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           79  GGCGCC               GGC^GCC
StuI         1203  AGGCCT               AGG^CCT
TspRI         154  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        663  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1089  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I        106  GTGCAC               G^TGCAC
              379  GTGCAC              
AlwI          960  GATCC                GGATC(4/5)
             1077  GGATC               
AlwNI         574  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              856  CAGGACCTG           
ApaLI         106  GTGCAC               G^TGCAC
              379  GTGCAC              
BsmBI         415  GAGACG               CGTCTC(1/5)
              865  GAGACG              
BsrFI         339  GCCGGC               [GA]^CCGG[CT]
              851  GCCGGC              
BssHII        684  GCGCGC               G^CGCGC
              686  GCGCGC              
BstXI         218  CCACCTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1242  CCAGGCTGGTGG        
BstYI         959  AGATCC               [GA]^GATC[CT]
             1077  GGATCT              
Cfr10I        339  GCCGGC               [GA]^CCGG[CT]
              851  GCCGGC              
DdeI          344  CTGAG                C^T[ACGT]AG
             1147  CTGAG               
HaeII          79  GGCGCC               [GA]GCGC^[CT]
              194  AGCGCT              
HgaI          417  GACGC                GACGC(5/10)
              576  GACGC               
KspI            5  CCGCGG               CCGC^GG
              703  CCGCGG              
MflI          959  AGATCC               [GA]^GATC[CT]
             1077  GGATCT              
NaeI          339  GCCGGC               GCC^GGC
              851  GCCGGC              
NgoMIV        339  GCCGGC               G^CCGGC
              851  GCCGGC              
NspI          209  ACATGT               [GA]CATG^[CT]
              245  ACATGC              
PpuMI         857  AGGACCT              [GA]G^G[AT]CC[CT]
             1256  GGGTCCT             
SacII           5  CCGCGG               CCGC^GG
              703  CCGCGG              
SapI          837  GCTCTTC              GCTCTTC(1/4)
              990  GCTCTTC             
XhoII         959  AGATCC               [GA]^GATC[CT]
             1077  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BalI        BanI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       DraII       DraIII      DsaI        
EaeI        EarI        EcoO109I    EcoO65I     EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        Tsp45I      TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com