Restriction map for pF1KB9865
 (Linear) (Six-base) MAPSORT of: KIBB9865.seq  Check: 9897  from: 1  to: 1296



KIBB9865.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     110     366    1296 
   Size:        110     256     930

  Fragments arranged by size: 

                 930     256     110
 
AfeI AGC'GCT

Cuts at:      0     196    1296 
   Size:        196    1100
 
AflIII A'CryG_T

Cuts at:      0     209    1296 
   Size:        209    1087
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186    1296 
   Size:        186    1110
 
AleI CACnn'nnGTG

Cuts at:      0     223    1296 
   Size:        223    1073
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    1296 
   Size:       1078      32     186

  Fragments arranged by size: 

                1078     186      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     579     861    1296 
   Size:        579     282     435

  Fragments arranged by size: 

                 579     435     282
 
ApaLI G'TGCA_C

Cuts at:      0     106     379    1296 
   Size:        106     273     917

  Fragments arranged by size: 

                 917     273     106
 
BanI G'GyrC_C

Cuts at:      0      79     122     532    1047    1296 
   Size:         79      43     410     515     249

  Fragments arranged by size: 

                 515     410     249      79      43
 
BanII G_rGCy'C

Cuts at:      0     986    1296 
   Size:        986     310
 
BbeI G_GCGC'C

Cuts at:      0      83    1296 
   Size:         83    1213
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     468    1296 
   Size:        468     828
 
BbvCI CC'TCA_GC

Cuts at:      0     344    1296 
   Size:        344     952
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     117     151    1296 
   Size:        117      34    1145

  Fragments arranged by size: 

                1145     117      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     541     550     604    1202    1296 
   Size:         16     525       9      54     598      94

  Fragments arranged by size: 

                 598     525      94      54      16       9
 
Bme1580I G_kGCm'C

Cuts at:      0     110     383     466     573    1052    1296 
   Size:        110     273      83     107     479     244

  Fragments arranged by size: 

                 479     273     244     110     107      83
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     486    1296 
   Size:        486     810
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1068    1296 
   Size:       1068     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     883    1225    1282    1296 
   Size:        355     528     342      57      14

  Fragments arranged by size: 

                 528     355     342      57      14
 
Bpu10I CC'TnA_GC

Cuts at:      0     344    1147    1296 
   Size:        344     803     149

  Fragments arranged by size: 

                 803     344     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1172    1296 
   Size:       1172     124
 
BsaBI GATnn'nnATC

Cuts at:      0     748    1296 
   Size:        748     548
 
BsaHI Gr'CG_yC

Cuts at:      0      80    1296 
   Size:         80    1216
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     709     739    1078    1108    1296 
   Size:        709      30     339      30     188

  Fragments arranged by size: 

                 709     339     188      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     472     730    1174    1296 
   Size:         61     411     258     444     122

  Fragments arranged by size: 

                 444     411     258     122      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43    1296 
   Size:         43    1253
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     383     992    1296 
   Size:        110     273     609     304

  Fragments arranged by size: 

                 609     304     273     110
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     409     859    1296 
   Size:        409     450     437

  Fragments arranged by size: 

                 450     437     409
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     383     466     573     986     992    1052    1296 
   Size:        110     273      83     107     413       6      60     244

  Fragments arranged by size: 

                 413     273     244     110     107      83      60       6
            
 
BspHI T'CATG_A

Cuts at:      0     524    1296 
   Size:        524     772
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     110     366     659    1296 
   Size:        110     256     293     637

  Fragments arranged by size: 

                 637     293     256     110
 
BsrBI CCG'CTC

Cuts at:      0     837    1296 
   Size:        837     459
 
BsrFI r'CCGG_y

Cuts at:      0     339     851    1296 
   Size:        339     512     445

  Fragments arranged by size: 

                 512     445     339
 
BssHII G'CGCG_C

Cuts at:      0     684     686    1296 
   Size:        684       2     610

  Fragments arranged by size: 

                 684     610       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     626    1296 
   Size:        626     670
 
BstEII G'GTnAC_C

Cuts at:      0      66     328     646     708     970    1296 
   Size:         66     262     318      62     262     326

  Fragments arranged by size: 

                 326     318     262     262      66      62
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     225    1249    1296 
   Size:        225    1024      47

  Fragments arranged by size: 

                1024     225      47
 
BstYI r'GATC_y

Cuts at:      0     959    1077    1296 
   Size:        959     118     219

  Fragments arranged by size: 

                 959     219     118
 
BtgI C'CryG_G

Cuts at:      0       5     599     703    1296 
   Size:          5     594     104     593

  Fragments arranged by size: 

                 594     593     104       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     297    1296 
   Size:        297     999
 
ClaI AT'CG_AT

Cuts at:      0     752    1296 
   Size:        752     544
 
DraIII CAC_nnn'GTG

Cuts at:      0     328     367     669    1296 
   Size:        328      39     302     627

  Fragments arranged by size: 

                 627     328     302      39
 
EaeI y'GGCC_r

Cuts at:      0       8     150     470     542     950    1188    1296 
   Size:          8     142     320      72     408     238     108

  Fragments arranged by size: 

                 408     320     238     142     108      72       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     409     844     997    1296 
   Size:        409     435     153     299

  Fragments arranged by size: 

                 435     409     299     153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1186    1296 
   Size:       1186     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     883    1225    1282    1296 
   Size:        355     528     342      57      14

  Fragments arranged by size: 

                 528     355     342      57      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     771     858    1257    1296 
   Size:        771      87     399      39

  Fragments arranged by size: 

                 771     399      87      39
 
FspI TGC'GCA

Cuts at:      0     655    1296 
   Size:        655     641
 
HaeII r_GCGC'y

Cuts at:      0      83     198    1296 
   Size:         83     115    1098

  Fragments arranged by size: 

                1098     115      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204    1296 
   Size:        172      32    1092

  Fragments arranged by size: 

                1092     172      32
 
KasI G'GCGC_C

Cuts at:      0      79    1296 
   Size:         79    1217
 
MscI TGG'CCA

Cuts at:      0     152     544     952    1296 
   Size:        152     392     408     344

  Fragments arranged by size: 

                 408     392     344     152
 
MslI CAynn'nnrTG

Cuts at:      0     223     316     484     754     919    1296 
   Size:        223      93     168     270     165     377

  Fragments arranged by size: 

                 377     270     223     168     165      93
 
MspA1I CmG'CkG

Cuts at:      0       7     236     705     735    1064    1269    1296 
   Size:          7     229     469      30     329     205      27

  Fragments arranged by size: 

                 469     329     229     205      30      27       7
 
NaeI GCC'GGC

Cuts at:      0     341     853    1296 
   Size:        341     512     443

  Fragments arranged by size: 

                 512     443     341
 
NarI GG'CG_CC

Cuts at:      0      80    1296 
   Size:         80    1216
 
NgoMIV G'CCGG_C

Cuts at:      0     339     851    1296 
   Size:        339     512     445

  Fragments arranged by size: 

                 512     445     339
 
NspI r_CATG'y

Cuts at:      0     213     249    1296 
   Size:        213      36    1047

  Fragments arranged by size: 

                1047     213      36
 
PasI CC'CwG_GG

Cuts at:      0    1106    1296 
   Size:       1106     190
 
PciI A'CATG_T

Cuts at:      0     209    1296 
   Size:        209    1087
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     669    1296 
   Size:        669     627
 
PfoI T'CCnGG_A

Cuts at:      0     893    1259    1296 
   Size:        893     366      37

  Fragments arranged by size: 

                 893     366      37
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    1296 
   Size:       1078      32     186

  Fragments arranged by size: 

                1078     186      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     858    1257    1296 
   Size:        858     399      39

  Fragments arranged by size: 

                 858     399      39
 
PshAI GACnn'nnGTC

Cuts at:      0    1284    1296 
   Size:       1284      12
 
PstI C_TGCA'G

Cuts at:      0    1069    1296 
   Size:       1069     227
 
SacII CC_GC'GG

Cuts at:      0       8     706    1296 
   Size:          8     698     590

  Fragments arranged by size: 

                 698     590       8
 
SapI GCTCTTCn'nnn_

Cuts at:      0     844     997    1296 
   Size:        844     153     299

  Fragments arranged by size: 

                 844     299     153
 
SexAI A'CCwGG_T

Cuts at:      0      62    1296 
   Size:         62    1234
 
SfcI C'TryA_G

Cuts at:      0      27    1065    1275    1296 
   Size:         27    1038     210      21

  Fragments arranged by size: 

                1038     210      27      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      16    1296 
   Size:         16    1280
 
SfoI GGC'GCC

Cuts at:      0      81    1296 
   Size:         81    1215
 
StuI AGG'CCT

Cuts at:      0    1205    1296 
   Size:       1205      91
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     943    1296 
   Size:        943     353
 
TatI w'GTAC_w

Cuts at:      0     725    1007    1296 
   Size:        725     282     289

  Fragments arranged by size: 

                 725     289     282
 
XmnI GAAnn'nnTTC

Cuts at:      0    1093    1296 
   Size:       1093     203
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AhdI     AleI     AloI    AlwNI    ApaLI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI Bme1580I
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaXI
    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI   BssHII   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     ClaI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I     FspI
    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI
    PshAI     PstI    SacII     SapI    SexAI     SfcI     SfiI     SfoI
     StuI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AlfI
     ApaI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
    BpuEI    BsaAI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BspEI
    BsrDI    BsrGI    BssSI    BstBI  BstZ17I   Bsu36I     BtsI    CspCI
     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SbfI     ScaI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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