Restriction map for pF1KB7265
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaI        
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MamI        MfeI        
MluI        MseI        MslI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        Tru9I       Tsp509I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        670  GGTACC               G^GTACC
AccII         611  CGCG                 CG^CG
AcyI          540  GACGCC               G[GA]^CG[CT]C
AlwNI         562  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       670  GGTACC               G^GTACC
AspI          916  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          759  TGGCCA               TGG^CCA
BanII         886  GAGCTC               G[GA]GC[CT]^C
BbsI          553  GAAGAC               GAAGAC(2/6)
BfaI          208  CTAG                 C^TAG
BglII         524  AGATCT               A^GATCT
BpuAI         553  GAAGAC               GAAGAC(2/6)
BsaHI         540  GACGCC               G[GA]^CG[CT]C
BsgI          685  GTGCAG               GTGCAG(16/14)
BsmI          856  GAATGC               GAATGC(1/-1)
BstUI         611  CGCG                 CG^CG
BstYI         524  AGATCT               [GA]^GATC[CT]
EaeI          759  TGGCCA               [CT]^GGCC[GA]
EcoRV         580  GATATC               GAT^ATC
HaeII         478  AGCGCC               [GA]GCGC^[CT]
Hin1I         540  GACGCC               G[GA]^CG[CT]C
KpnI          670  GGTACC               GGTAC^C
MaeI          208  CTAG                 C^TAG
MaeII         839  ACGT                 A^CGT
MflI          524  AGATCT               [GA]^GATC[CT]
MluNI         759  TGGCCA               TGG^CCA
MscI          759  TGGCCA               TGG^CCA
MspA1I        483  CAGCTG               C[AC]G^C[GT]G
MvnI          611  CGCG                 CG^CG
NaeI          488  GCCGGC               GCC^GGC
NgoMIV        488  GCCGGC               G^CCGGC
NspI           98  ACATGC               [GA]CATG^[CT]
PflFI         916  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         483  CAGCTG               CAG^CTG
SacI          886  GAGCTC               GAGCT^C
SfcI          831  CTACAG               C^T[GA][CT]AG
StuI          827  AGGCCT               AGG^CCT
TfiI          146  GAATC                G^A[AT]TC
TspRI         469  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       916  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         524  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII        270  TCCGGA               T^CCGGA
              923  TCCGGA              
Asp700I        15  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              522  GAAGATCTTC          
AspHI         886  GAGCTC               G[AT]GC[AT]^C
              931  GAGCAC              
BcnI           92  CCCGG                CC^[GC]GG
              416  CCGGG               
BmyI          886  GAGCTC               G[AGT]GC[ACT]^C
              931  GAGCAC              
BpmI          403  CTGGAG               CTGGAG(16/14)
              910  CTGGAG              
BsaWI         270  TCCGGA               [AT]^CCGG[AT]
              923  TCCGGA              
BseAI         270  TCCGGA               T^CCGGA
              923  TCCGGA              
BseRI         655  CTCCTC               GAGGAG(10/8)
              800  GAGGAG              
BsiHKAI       886  GAGCTC               G[AT]GC[AT]^C
              931  GAGCAC              
Bsp1286I      886  GAGCTC               G[AGT]GC[ACT]^C
              931  GAGCAC              
BspEI         270  TCCGGA               T^CCGGA
              923  TCCGGA              
BspMI        1011  ACCTGC               ACCTGC(4/8)
              681  GCAGGT              
BsrFI         190  ACCGGC               [GA]^CCGG[CT]
              488  GCCGGC              
BsrI          762  CCAGT                ACTGG(1/-1)
              951  CCAGT               
Cfr10I        190  ACCGGC               [GA]^CCGG[CT]
              488  GCCGGC              
DpnII         249  GATC                 ^GATC
              525  GATC                
DraII         914  AGGACCT              [GA]G^G[ACGT]CC[CT]
              965  GGGTCCC             
EcoNI         288  CCTCGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              348  CCTGCTGAAGG         
EcoO109I      914  AGGACCT              [GA]G^G[ACGT]CC[CT]
              965  GGGTCCC             
MaeIII        332  GTCAC                ^GT[ACGT]AC
              538  GTGAC               
MboI          249  GATC                 ^GATC
              525  GATC                
MroI          270  TCCGGA               T^CCGGA
              923  TCCGGA              
NciI           92  CCCGG                CC^[GC]GG
              416  CCGGG               
NdeII         249  GATC                 ^GATC
              525  GATC                
PleI          817  GACTC                GAGTC(4/5)
              122  GAGTC               
PpuMI         914  AGGACCT              [GA]G^G[AT]CC[CT]
              965  GGGTCCC             
Sau3AI        249  GATC                 ^GATC
              525  GATC                
SfaNI         946  GATGC                GCATC(5/9)
              164  GCATC               
Tsp45I        332  GTCAC                ^GT[GC]AC
              538  GTGAC               
XmnI           15  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              522  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BbvI        BlpI        Bpu1102I    BsaJI       BsiYI       
BslI        BsmFI       BssKI       BstF5I      BstNI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MboII       
MnlI        MspI        MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       StyI        
TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com