Restriction map for pF1KB7265
 (Linear) (Six-base) MAPSORT of: KIBB7265.seq  Check: 6626  from: 1  to: 1056



KIBB7265.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     672    1020    1056 
   Size:        672     348      36

  Fragments arranged by size: 

                 672     348      36
 
Acc65I G'GTAC_C

Cuts at:      0     670    1056 
   Size:        670     386
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     664     968    1056 
   Size:        373     291     304      88

  Fragments arranged by size: 

                 373     304     291      88
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     710    1056 
   Size:        710     346
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       7      39    1056 
   Size:          7      32    1017

  Fragments arranged by size: 

                1017      32       7
 
AlwNI CAG_nnn'CTG

Cuts at:      0     567    1056 
   Size:        567     489
 
BanI G'GyrC_C

Cuts at:      0     451     670     714    1056 
   Size:        451     219      44     342

  Fragments arranged by size: 

                 451     342     219      44
 
BanII G_rGCy'C

Cuts at:      0     890    1056 
   Size:        890     166
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     560    1056 
   Size:        560     496
 
BglII A'GATC_T

Cuts at:      0     524    1056 
   Size:        524     532
 
BlpI GC'TnA_GC

Cuts at:      0      34     505     884    1056 
   Size:         34     471     379     172

  Fragments arranged by size: 

                 471     379     172      34
 
BmgBI CAC'GTC

Cuts at:      0     840    1056 
   Size:        840     216
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     945    1056 
   Size:        945     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     931    1056 
   Size:        424     507     125

  Fragments arranged by size: 

                 507     424     125
 
BsaHI Gr'CG_yC

Cuts at:      0     541    1056 
   Size:        541     515
 
BsaWI w'CCGG_w

Cuts at:      0     270     923    1056 
   Size:        270     653     133

  Fragments arranged by size: 

                 653     270     133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       7      37     802     832    1056 
   Size:          7      30     765      30     224

  Fragments arranged by size: 

                 765     224      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     646     815    1056 
   Size:        646     169     241

  Fragments arranged by size: 

                 646     241     169
 
BseYI C'CCAG_C

Cuts at:      0     606     849     976    1056 
   Size:        606     243     127      80

  Fragments arranged by size: 

                 606     243     127      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1056 
   Size:        706     350
 
BsiHKAI G_wGCw'C

Cuts at:      0     890     935    1056 
   Size:        890      45     121

  Fragments arranged by size: 

                 890     121      45
 
BsmI GAATG_Cn'

Cuts at:      0     862    1056 
   Size:        862     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     890     935    1056 
   Size:        890      45     121

  Fragments arranged by size: 

                 890     121      45
 
BspEI T'CCGG_A

Cuts at:      0     270     923    1056 
   Size:        270     653     133

  Fragments arranged by size: 

                 653     270     133
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     672    1020    1056 
   Size:        672     348      36

  Fragments arranged by size: 

                 672     348      36
 
BsrFI r'CCGG_y

Cuts at:      0     190     488    1056 
   Size:        190     298     568

  Fragments arranged by size: 

                 568     298     190
 
BstYI r'GATC_y

Cuts at:      0     524    1056 
   Size:        524     532
 
BtsI GCAGTG_nn'

Cuts at:      0     468    1056 
   Size:        468     588
 
EaeI y'GGCC_r

Cuts at:      0     759    1056 
   Size:        759     297
 
EcoICRI GAG'CTC

Cuts at:      0     888    1056 
   Size:        888     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     424     664     931     968    1056 
   Size:        373      51     240     267      37      88

  Fragments arranged by size: 

                 373     267     240      88      51      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     292     352    1056 
   Size:        292      60     704

  Fragments arranged by size: 

                 704     292      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     915     966    1056 
   Size:        915      51      90

  Fragments arranged by size: 

                 915      90      51
 
EcoRV GAT'ATC

Cuts at:      0     582    1056 
   Size:        582     474
 
HaeII r_GCGC'y

Cuts at:      0     482    1056 
   Size:        482     574
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     131     163     195     802     834     937     969    1056 
   Size:        131      32      32     607      32     103      32      87

  Fragments arranged by size: 

                 607     131     103      87      32      32      32      32
            
 
KpnI G_GTAC'C

Cuts at:      0     674    1056 
   Size:        674     382
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1056 
   Size:        746     310
 
MscI TGG'CCA

Cuts at:      0     761    1056 
   Size:        761     295
 
MspA1I CmG'CkG

Cuts at:      0     485    1056 
   Size:        485     571
 
NaeI GCC'GGC

Cuts at:      0     490    1056 
   Size:        490     566
 
NgoMIV G'CCGG_C

Cuts at:      0     488    1056 
   Size:        488     568
 
NspI r_CATG'y

Cuts at:      0     102    1056 
   Size:        102     954
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39    1056 
   Size:          7      32    1017

  Fragments arranged by size: 

                1017      32       7
 
PpuMI rG'GwC_Cy

Cuts at:      0     915     966    1056 
   Size:        915      51      90

  Fragments arranged by size: 

                 915      90      51
 
PvuII CAG'CTG

Cuts at:      0     485    1056 
   Size:        485     571
 
SacI G_AGCT'C

Cuts at:      0     890    1056 
   Size:        890     166
 
SanDI GG'GwC_CC

Cuts at:      0     966    1056 
   Size:        966      90
 
SfcI C'TryA_G

Cuts at:      0     831    1056 
   Size:        831     225
 
StuI AGG'CCT

Cuts at:      0     829    1056 
   Size:        829     227
 
StyI C'CwwG_G

Cuts at:      0      77     293     891    1016    1056 
   Size:         77     216     598     125      40

  Fragments arranged by size: 

                 598     216     125      77      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     841    1056 
   Size:        841     215
 
Tth111I GACn'n_nGTC

Cuts at:      0     919    1056 
   Size:        919     137
 
XmnI GAAnn'nnTTC

Cuts at:      0      19     526    1056 
   Size:         19     507     530

  Fragments arranged by size: 

                 530     507      19
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI     AloI    AlwNI     BanI    BanII
     BbsI    BglII     BlpI    BmgBI     BmrI     BpmI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrFI    BstYI     BtsI     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV    HaeII    Hin4I     KpnI     MmeI     MscI   MspA1I
     NaeI   NgoMIV     NspI     PpiI    PpuMI    PvuII     SacI    SanDI
     SfcI     StuI     StyI    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI Bme1580I     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoRI     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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