Restriction map for pF1KB8168
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MseI        
MunI        NaeI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        225  GGTACC               G^GTACC
AccIII        669  TCCGGA               T^CCGGA
AgeI          714  ACCGGT               A^CCGGT
Asp718I       225  GGTACC               G^GTACC
AvaI           16  CCCGAG               C^[CT]CG[GA]G
BalI          143  TGGCCA               TGG^CCA
BlpI          256  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      256  GCTAAGC              GC^T[ACGT]AGC
BseAI         669  TCCGGA               T^CCGGA
BsmI          385  GAATGC               GAATGC(1/-1)
BsoBI          16  CCCGAG               C^[CT]CG[GA]G
BspEI         669  TCCGGA               T^CCGGA
BsrBI         504  GAGCGG               CCGCTC(-3/-3)
BsrFI         714  ACCGGT               [GA]^CCGG[CT]
BstAPI        394  GCAGAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        729  GGTCACC              G^GT[ACGT]ACC
BstF5I        667  CATCC                GGATG(2/0)
BstPI         729  GGTCACC              G^GT[ACGT]ACC
BstXI          53  CCATCGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         256  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        714  ACCGGT               [GA]^CCGG[CT]
CpoI          711  CGGACCG              CG^G[AT]CCG
EcoO65I       729  GGTCACC              G^GT[ACGT]ACC
FokI          667  CATCC                GGATG(9/13)
HgaI          340  GACGC                GACGC(5/10)
KpnI          225  GGTACC               GGTAC^C
MluNI         143  TGGCCA               TGG^CCA
MroI          669  TCCGGA               T^CCGGA
MscI          143  TGGCCA               TGG^CCA
MslI           54  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        114  CATG                 CATG^
PflMI         135  CCAGATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         714  ACCGGT               A^CCGGT
PstI          280  CTGCAG               CTGCA^G
RsrII         711  CGGACCG              CG^G[AT]CCG
SacI            8  GAGCTC               GAGCT^C
SmlI           93  CTTGAG               C^T[CT][GA]AG
Tsp45I        730  GTCAC                ^GT[GC]AC
Tsp509I       168  AATT                 ^AATT
Van91I        135  CCAGATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          508  GGCGCC               G[GA]^CG[CT]C
              675  GGCGCC              
AfaI           21  GTAC                 GT^AC
              226  GTAC                
AspHI           8  GAGCTC               G[AT]GC[AT]^C
              718  GTGCTC              
AvaII         542  GGACC                G^G[AT]CC
              712  GGACC               
BbeI          508  GGCGCC               GGCGC^C
              675  GGCGCC              
BbsI          547  GTCTTC               GAAGAC(2/6)
              787  GTCTTC              
BpuAI         547  GTCTTC               GAAGAC(2/6)
              787  GTCTTC              
BsaHI         508  GGCGCC               G[GA]^CG[CT]C
              675  GGCGCC              
BsaI           49  GAGACC               GGTCTC(1/5)
              185  GAGACC              
BseRI          27  CTCCTC               GAGGAG(10/8)
              765  CTCCTC              
BsiHKAI         8  GAGCTC               G[AT]GC[AT]^C
              718  GTGCTC              
BsrI          459  ACTGG                ACTGG(1/-1)
              230  CCAGT               
BstYI         250  GGATCT               [GA]^GATC[CT]
              275  AGATCC              
EaeI          143  TGGCCA               [CT]^GGCC[GA]
              597  CGGCCA              
EcoT14I       867  CCTTGG               C^C[AT][AT]GG
              886  CCTTGG              
Hin1I         508  GGCGCC               G[GA]^CG[CT]C
              675  GGCGCC              
KasI          508  GGCGCC               G^GCGCC
              675  GGCGCC              
MaeIII        332  GTTAC                ^GT[ACGT]AC
              730  GTCAC               
MflI          250  GGATCT               [GA]^GATC[CT]
              275  AGATCC              
MspA1I        490  CAGCGG               C[AC]G^C[GT]G
              577  CCGCTG              
NarI          508  GGCGCC               GG^CGCC
              675  GGCGCC              
RsaI           21  GTAC                 GT^AC
              226  GTAC                
SfcI          280  CTGCAG               C^T[GA][CT]AG
              879  CTATAG              
SfoI          508  GGCGCC               GGC^GCC
              675  GGCGCC              
StyI          867  CCTTGG               C^C[AT][AT]GG
              886  CCTTGG              
XhoII         250  GGATCT               [GA]^GATC[CT]
              275  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BanI        
BanII       BbvI        BfaI        BmyI        BsaJI       
BsaWI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeI        MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     
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