Restriction map for pF1KB8168
 (Linear) (Six-base) MAPSORT of: KIBB8168.seq  Check: 9658  from: 1  to: 912



KIBB8168.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

Acc65I G'GTAC_C

Cuts at:      0     225     912 
   Size:        225     687
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827     912 
   Size:        827      85
 
AgeI A'CCGG_T

Cuts at:      0     714     912 
   Size:        714     198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     269     368     912 
   Size:        269      99     544

  Fragments arranged by size: 

                 544     269      99
 
AvaI C'yCGr_G

Cuts at:      0      16     912 
   Size:         16     896
 
BanI G'GyrC_C

Cuts at:      0     225     508     613     675     912 
   Size:        225     283     105      62     237

  Fragments arranged by size: 

                 283     237     225     105      62
 
BanII G_rGCy'C

Cuts at:      0      12     661     841     912 
   Size:         12     649     180      71

  Fragments arranged by size: 

                 649     180      71      12
 
BbeI G_GCGC'C

Cuts at:      0     512     679     912 
   Size:        512     167     233

  Fragments arranged by size: 

                 512     233     167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     540     780     912 
   Size:        540     240     132

  Fragments arranged by size: 

                 540     240     132
 
BlpI GC'TnA_GC

Cuts at:      0     257     912 
   Size:        257     655
 
Bme1580I G_kGCm'C

Cuts at:      0     616     912 
   Size:        616     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     912 
   Size:        224     688
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      42     119     912 
   Size:         42      77     793

  Fragments arranged by size: 

                 793      77      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     912 
   Size:        114     798
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     179     912 
   Size:         43     136     733

  Fragments arranged by size: 

                 733     136      43
 
BsaHI Gr'CG_yC

Cuts at:      0     509     676     912 
   Size:        509     167     236

  Fragments arranged by size: 

                 509     236     167
 
BsaWI w'CCGG_w

Cuts at:      0     188     669     714     912 
   Size:        188     481      45     198

  Fragments arranged by size: 

                 481     198     188      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     704     734     912 
   Size:        704      30     178

  Fragments arranged by size: 

                 704     178      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     756     912 
   Size:         18     738     156

  Fragments arranged by size: 

                 738     156      18
 
BseYI C'CCAG_C

Cuts at:      0     488     654     912 
   Size:        488     166     258

  Fragments arranged by size: 

                 488     258     166
 
BsiHKAI G_wGCw'C

Cuts at:      0      12     722     912 
   Size:         12     710     190

  Fragments arranged by size: 

                 710     190      12
 
BsmI GAATG_Cn'

Cuts at:      0     391     912 
   Size:        391     521
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     616     661     722     841     912 
   Size:         12     604      45      61     119      71

  Fragments arranged by size: 

                 604     119      71      61      45      12
 
BspEI T'CCGG_A

Cuts at:      0     669     912 
   Size:        669     243
 
BsrBI CCG'CTC

Cuts at:      0     506     912 
   Size:        506     406
 
BsrFI r'CCGG_y

Cuts at:      0     714     912 
   Size:        714     198
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     400     912 
   Size:        400     512
 
BstEII G'GTnAC_C

Cuts at:      0     729     912 
   Size:        729     183
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      60     912 
   Size:         60     852
 
BstYI r'GATC_y

Cuts at:      0     250     275     912 
   Size:        250      25     637

  Fragments arranged by size: 

                 637     250      25
 
BtgI C'CryG_G

Cuts at:      0     290     600     617     629     912 
   Size:        290     310      17      12     283

  Fragments arranged by size: 

                 310     290     283      17      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     552     912 
   Size:        552     360
 
EaeI y'GGCC_r

Cuts at:      0     143     597     912 
   Size:        143     454     315

  Fragments arranged by size: 

                 454     315     143
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     726     912 
   Size:        726     186
 
EcoICRI GAG'CTC

Cuts at:      0      10     912 
   Size:         10     902
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827     912 
   Size:        827      85
 
HaeII r_GCGC'y

Cuts at:      0     180     512     679     694     912 
   Size:        180     332     167      15     218

  Fragments arranged by size: 

                 332     218     180     167      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208     279     311     704     736     912 
   Size:        176      32      71      32     393      32     176

  Fragments arranged by size: 

                 393     176     176      71      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     508     675     912 
   Size:        508     167     237

  Fragments arranged by size: 

                 508     237     167
 
KpnI G_GTAC'C

Cuts at:      0     229     912 
   Size:        229     683
 
MscI TGG'CCA

Cuts at:      0     145     912 
   Size:        145     767
 
MslI CAynn'nnrTG

Cuts at:      0      58     912 
   Size:         58     854
 
MspA1I CmG'CkG

Cuts at:      0     492     579     912 
   Size:        492      87     333

  Fragments arranged by size: 

                 492     333      87
 
NarI GG'CG_CC

Cuts at:      0     509     676     912 
   Size:        509     167     236

  Fragments arranged by size: 

                 509     236     167
 
PasI CC'CwG_GG

Cuts at:      0     536     912 
   Size:        536     376
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     141     912 
   Size:        141     771
 
PstI C_TGCA'G

Cuts at:      0     284     912 
   Size:        284     628
 
RsrII CG'GwC_CG

Cuts at:      0     712     912 
   Size:        712     200
 
SacI G_AGCT'C

Cuts at:      0      12     912 
   Size:         12     900
 
SfcI C'TryA_G

Cuts at:      0     280     879     912 
   Size:        280     599      33

  Fragments arranged by size: 

                 599     280      33
 
SfoI GGC'GCC

Cuts at:      0     510     677     912 
   Size:        510     167     235

  Fragments arranged by size: 

                 510     235     167
 
SmlI C'TyrA_G

Cuts at:      0      93     912 
   Size:         93     819
 
StyI C'CwwG_G

Cuts at:      0     867     886     912 
   Size:        867      19      26

  Fragments arranged by size: 

                 867      26      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     642     774     864     912 
   Size:        642     132      90      48

  Fragments arranged by size: 

                 642     132      90      48
 
TatI w'GTAC_w

Cuts at:      0      20     912 
   Size:         20     892
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AlfI     AvaI     BanI    BanII     BbeI
     BbsI     BlpI Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI
    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     EaeI     EciI  EcoICRI  Eco57MI    HaeII    Hin4I     KasI     KpnI
     MscI     MslI   MspA1I     NarI     PasI    PflMI     PstI    RsrII
     SacI     SfcI     SfoI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BpmI   Bpu10I    BsaAI
    BsaBI     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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