Restriction map for pF1KB8135
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AgeI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BamHI       BbvCI       BcgI        
BciVI       BclI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsmBI       
BspDI       BspEI       BspHI       BspMI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eco47III    Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
KpnI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        TaqI        TfiI        
Tsp509I     TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         901  GACGTC               GACGT^C
AflII         663  CTTAAG               C^TTAAG
AflIII        174  ACATGT               A^C[GA][CT]GT
AhdI          844  GACCGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1338  GTGCAC               G^TGCAC
ApaLI        1338  GTGCAC               G^TGCAC
AspEI         844  GACCGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1338  GTGCAC               G[AT]GC[AT]^C
BalI          104  TGGCCA               TGG^CCA
BanII          10  GAGCCC               G[GA]GC[CT]^C
BbeI           31  GGCGCC               GGCGC^C
BbrPI        1456  CACGTG               CAC^GTG
BfaI         1162  CTAG                 C^TAG
BfrI          663  CTTAAG               C^TTAAG
BglI          775  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1129  CTGGAG               CTGGAG(16/14)
BsaAI        1456  CACGTG               [CT]AC^GT[GA]
BsaI          271  GAGACC               GGTCTC(1/5)
BseRI         323  CTCCTC               GAGGAG(10/8)
BsgI          937  GTGCAG               GTGCAG(16/14)
BsiHKAI      1338  GTGCAC               G[AT]GC[AT]^C
BsiWI         408  CGTACG               C^GTACG
BsmI          560  GCATTC               GAATGC(1/-1)
Bsp1407I      437  TGTACA               T^GTACA
BspLU11I      174  ACATGT               A^CATGT
BsrBI        1288  GAGCGG               CCGCTC(-3/-3)
BsrDI         764  CATTGC               GCAATG(2/0)
BsrFI        1342  ACCGGC               [GA]^CCGG[CT]
BsrGI         437  TGTACA               T^GTACA
BstAPI       1340  GCACCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1342  ACCGGC               [GA]^CCGG[CT]
Eam1105I      844  GACCGAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         591  CCTCATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       521  CCATGG               C^C[AT][AT]GG
HaeII          31  GGCGCC               [GA]GCGC^[CT]
HpaI         1309  GTTAAC               GTT^AAC
KasI           31  GGCGCC               G^GCGCC
MaeI         1162  CTAG                 C^TAG
MluNI         104  TGGCCA               TGG^CCA
MscI          104  TGGCCA               TGG^CCA
NarI           31  GGCGCC               GG^CGCC
NcoI          521  CCATGG               C^CATGG
NdeI         1410  CATATG               CA^TATG
PmaCI        1456  CACGTG               CAC^GTG
PmlI         1456  CACGTG               CAC^GTG
PstI          122  CTGCAG               CTGCA^G
SapI          530  GAAGAGC              GCTCTTC(1/4)
SfcI          122  CTGCAG               C^T[GA][CT]AG
SfoI           31  GGCGCC               GGC^GCC
SmaI           13  CCCGGG               CCC^GGG
SmlI          663  CTTAAG               C^T[CT][GA]AG
SphI         1230  GCATGC               GCATG^C
SspBI         437  TGTACA               T^GTACA
StyI          521  CCATGG               C^C[AT][AT]GG
XmaCI          13  CCCGGG               C^CCGGG
XmaI           13  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI           31  GGCGCC               G[GA]^CG[CT]C
              901  GACGTC              
AspI          127  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1447  GACGAAGTC           
BsaHI          31  GGCGCC               G[GA]^CG[CT]C
              901  GACGTC              
BsiEI         462  CGGCCG               CG[GA][CT]^CG
             1317  CGGCCG              
BsmAI         271  GAGAC                GTCTC(1/5)
              321  GTCTC               
BstEII         87  GGTGACC              G^GT[ACGT]ACC
             1482  GGTGACC             
BstPI          87  GGTGACC              G^GT[ACGT]ACC
             1482  GGTGACC             
BstXI         140  CCACGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              249  CCACACAGATGG        
BstYI         605  GGATCT               [GA]^GATC[CT]
             1271  AGATCC              
EagI          462  CGGCCG               C^GGCCG
             1317  CGGCCG              
EarI          530  GAAGAG               CTCTTC(1/4)
             1165  GAAGAG              
EclXI         462  CGGCCG               C^GGCCG
             1317  CGGCCG              
Eco52I        462  CGGCCG               C^GGCCG
             1317  CGGCCG              
EcoO65I        87  GGTGACC              G^GT[ACGT]ACC
             1482  GGTGACC             
FauI         1111  CCCGC                CCCGC(4/6)
              571  GCGGG               
Hin1I          31  GGCGCC               G[GA]^CG[CT]C
              901  GACGTC              
Ksp632I       530  GAAGAG               CTCTTC(1/4)
             1165  GAAGAG              
KspI          779  CCGCGG               CCGC^GG
             1144  CCGCGG              
MflI          605  GGATCT               [GA]^GATC[CT]
             1271  AGATCC              
NspI          174  ACATGT               [GA]CATG^[CT]
             1230  GCATGC              
PflFI         127  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1447  GACGAAGTC           
PpuMI         312  AGGTCCT              [GA]G^G[AT]CC[CT]
              377  GGGACCC             
SacII         779  CCGCGG               CCGC^GG
             1144  CCGCGG              
Tth111I       127  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1447  GACGAAGTC           
XcmI           92  CCACAGTGACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              348  CCAAGAAAAAGGTGG     
XhoII         605  GGATCT               [GA]^GATC[CT]
             1271  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BanI        BbsI        
BbvI        BcnI        BmyI        BpuAI       BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       Tru9I       TseI        Tsp45I      TspRI       
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