Restriction map for pF1KB9808
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BseRI       BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluNI       
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TfiI        
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccII         184  CGCG                 CG^CG
AccIII        668  TCCGGA               T^CCGGA
AcsI          394  GAATTC               [GA]^AATT[CT]
AflIII        183  ACGCGT               A^C[GA][CT]GT
AlwI          303  GGATC                GGATC(4/5)
AlwNI         301  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          394  GAATTC               [GA]^AATT[CT]
AspHI         694  GTGCTC               G[AT]GC[AT]^C
AspI          409  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          563  GGTGCC               G^G[CT][GA]CC
BanII         149  GAGCCC               G[GA]GC[CT]^C
BclI           23  TGATCA               T^GATCA
BlpI          696  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      696  GCTCAGC              GC^T[ACGT]AGC
BsaI          526  GAGACC               GGTCTC(1/5)
BsaWI         668  TCCGGA               [AT]^CCGG[AT]
BseAI         668  TCCGGA               T^CCGGA
BsgI          179  CTGCAC               GTGCAG(16/14)
BsiHKAI       694  GTGCTC               G[AT]GC[AT]^C
BsmAI         526  GAGAC                GTCTC(1/5)
BsmI          123  GCATTC               GAATGC(1/-1)
BspEI         668  TCCGGA               T^CCGGA
BsrDI          71  GCAATG               GCAATG(2/0)
BstUI         184  CGCG                 CG^CG
BstYI         303  GGATCT               [GA]^GATC[CT]
CelII         696  GCTCAGC              GC^T[ACGT]AGC
DraI          229  TTTAAA               TTT^AAA
DraII         539  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        251  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          310  GACTTCGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          635  CCATGG               C^C[GA][CT]GG
EarI          716  GAAGAG               CTCTTC(1/4)
EcoNI         335  CCTTCAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      539  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         394  GAATTC               G^AATTC
EcoT14I       635  CCATGG               C^C[AT][AT]GG
FbaI           23  TGATCA               T^GATCA
HaeIII        681  GGCC                 GG^CC
HincII        553  GTTGAC               GT[CT]^[GA]AC
HindII        553  GTTGAC               GT[CT]^[GA]AC
Ksp632I       716  GAAGAG               CTCTTC(1/4)
MaeIII        407  GTGAC                ^GT[ACGT]AC
MflI          303  GGATCT               [GA]^GATC[CT]
MluI          183  ACGCGT               A^CGCGT
MroI          668  TCCGGA               T^CCGGA
MslI          207  CATTACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          184  CGCG                 CG^CG
NcoI          635  CCATGG               C^CATGG
PflFI         409  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         539  GGGACCC              [GA]G^G[AT]CC[CT]
SapI          716  GAAGAGC              GCTCTTC(1/4)
SbfI           79  CCTGCAGG             CCTGCA^GG
ScaI          515  AGTACT               AGT^ACT
Sse8387I       79  CCTGCAGG             CCTGCA^GG
StyI          635  CCATGG               C^C[AT][AT]GG
TaqI          314  TCGA                 T^CGA
Tsp45I        407  GTGAC                ^GT[GC]AC
Tsp509I       395  AATT                 ^AATT
Tth111I       409  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       314  TCGA                 T^CGA
XhoII         303  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          516  GTAC                 GT^AC
              602  GTAC                
AvaII         216  GGACC                G^G[AT]CC
              540  GGACC               
BmyI          149  GAGCCC               G[AGT]GC[ACT]^C
              694  GTGCTC              
BsmFI         539  GGGAC                GGGAC(10/14)
               76  GTCCC               
Bsp1286I      149  GAGCCC               G[AGT]GC[ACT]^C
              694  GTGCTC              
BsrI          107  ACTGG                ACTGG(1/-1)
              587  ACTGG               
BstNI         221  CCAGG                CC^[AT]GG
              656  CCTGG               
CfoI          158  GCGC                 GCG^C
              628  GCGC                
Cfr13I        216  GGACC                G^G[ACGT]CC
              540  GGACC               
EcoRII        221  CCAGG                ^CC[AT]GG
              656  CCTGG               
HaeII         157  AGCGCC               [GA]GCGC^[CT]
              627  GGCGCT              
HhaI          158  GCGC                 GCG^C
              628  GCGC                
HinP1I        158  GCGC                 G^CGC
              628  GCGC                
MboII         716  GAAGA                GAAGA(8/7)
              380  TCTTC               
MseI          230  TTAA                 T^TAA
              494  TTAA                
MspA1I        167  CAGCGG               C[AC]G^C[GT]G
              558  CAGCGG              
MvaI          221  CCAGG                CC^[AT]GG
              656  CCTGG               
NlaIII          6  CATG                 CATG^
              636  CATG                
PstI           80  CTGCAG               CTGCA^G
              290  CTGCAG              
RsaI          516  GTAC                 GT^AC
              602  GTAC                
Sau96I        216  GGACC                G^G[ACGT]CC
              540  GGACC               
SfcI           80  CTGCAG               C^T[GA][CT]AG
              290  CTGCAG              
Tru9I         230  TTAA                 T^TAA
              494  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      Cac8I       
DdeI        DpnII       FauI        Fnu4HI      FokI        
HapII       HinfI       HpaII       HphI        ItaI        
MboI        MnlI        MspI        MwoI        NciI        
NdeII       NlaIV       PleI        Sau3AI      ScrFI       
TseI        TspRI       
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