Restriction map for pF1KB9808
 (Linear) (Six-base) MAPSORT of: KIBB9808.seq  Check: 924  from: 1  to: 723



KIBB9808.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     723 
   Size:        103     620
 
AflIII A'CryG_T

Cuts at:      0     183     723 
   Size:        183     540
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     723 
   Size:        306     417
 
ApoI r'AATT_y

Cuts at:      0     394     723 
   Size:        394     329
 
BanI G'GyrC_C

Cuts at:      0     563     723 
   Size:        563     160
 
BanII G_rGCy'C

Cuts at:      0     153     723 
   Size:        153     570
 
BclI T'GATC_A

Cuts at:      0      23     723 
   Size:         23     700
 
BlpI GC'TnA_GC

Cuts at:      0     697     723 
   Size:        697      26
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     520     723 
   Size:        520     203
 
BsaWI w'CCGG_w

Cuts at:      0     668     723 
   Size:        668      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     261     291     723 
   Size:        261      30     432

  Fragments arranged by size: 

                 432     261      30
 
BseYI C'CCAG_C

Cuts at:      0     272     723 
   Size:        272     451
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     723 
   Size:        164     559
 
BsiHKAI G_wGCw'C

Cuts at:      0     698     723 
   Size:        698      25
 
BsmI GAATG_Cn'

Cuts at:      0     123     723 
   Size:        123     600
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     698     723 
   Size:        153     545      25

  Fragments arranged by size: 

                 545     153      25
 
BspEI T'CCGG_A

Cuts at:      0     668     723 
   Size:        668      55
 
BsrDI GCAATG_nn'

Cuts at:      0      78     723 
   Size:         78     645
 
BstYI r'GATC_y

Cuts at:      0     303     723 
   Size:        303     420
 
BtgI C'CryG_G

Cuts at:      0     635     723 
   Size:        635      88
 
BtsI GCAGTG_nn'

Cuts at:      0     134     723 
   Size:        134     589
 
DraI TTT'AAA

Cuts at:      0     231     723 
   Size:        231     492
 
DraIII CAC_nnn'GTG

Cuts at:      0     256     723 
   Size:        256     467
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     316     723 
   Size:        316     407
 
EarI CTCTTCn'nnn_

Cuts at:      0     711     723 
   Size:        711      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      67     723 
   Size:         67     656
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     723 
   Size:        103     620
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     339     723 
   Size:        339     384
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     723 
   Size:        540     183
 
EcoRI G'AATT_C

Cuts at:      0     394     723 
   Size:        394     329
 
HaeII r_GCGC'y

Cuts at:      0     161     631     723 
   Size:        161     470      92

  Fragments arranged by size: 

                 470     161      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      77     109     723 
   Size:         77      32     614

  Fragments arranged by size: 

                 614      77      32
 
HincII GTy'rAC

Cuts at:      0     555     723 
   Size:        555     168
 
MluI A'CGCG_T

Cuts at:      0     183     723 
   Size:        183     540
 
MslI CAynn'nnrTG

Cuts at:      0     211     723 
   Size:        211     512
 
MspA1I CmG'CkG

Cuts at:      0     169     560     723 
   Size:        169     391     163

  Fragments arranged by size: 

                 391     169     163
 
NcoI C'CATG_G

Cuts at:      0     635     723 
   Size:        635      88
 
PpuMI rG'GwC_Cy

Cuts at:      0     540     723 
   Size:        540     183
 
PstI C_TGCA'G

Cuts at:      0      84     294     723 
   Size:         84     210     429

  Fragments arranged by size: 

                 429     210      84
 
SanDI GG'GwC_CC

Cuts at:      0     540     723 
   Size:        540     183
 
SapI GCTCTTCn'nnn_

Cuts at:      0     711     723 
   Size:        711      12
 
SbfI CC_TGCA'GG

Cuts at:      0      84     723 
   Size:         84     639
 
ScaI AGT'ACT

Cuts at:      0     517     723 
   Size:        517     206
 
SfcI C'TryA_G

Cuts at:      0      80     290     723 
   Size:         80     210     433

  Fragments arranged by size: 

                 433     210      80
 
StyI C'CwwG_G

Cuts at:      0     635     723 
   Size:        635      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     286     723 
   Size:        286     437
 
TatI w'GTAC_w

Cuts at:      0     515     723 
   Size:        515     208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     261     293     723 
   Size:        261      32     430

  Fragments arranged by size: 

                 430     261      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     412     723 
   Size:        412     311
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     BanI    BanII     BclI     BlpI
     BsaI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BsrDI    BstYI     BtgI     BtsI     DraI   DraIII     DrdI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     MluI     MslI   MspA1I     NcoI    PpuMI     PstI    SanDI
     SapI     SbfI     ScaI     SfcI     StyI    TaqII     TatI     TstI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BseRI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     EaeI     EagI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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