Restriction map for pF1KB5337
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BsmFI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       Bst1107I    BstAPI      
BstBI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HinfI       HpaI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII         107  CGCG                 CG^CG
AcsI          434  AAATTC               [GA]^AATT[CT]
AcyI          480  GGCGCC               G[GA]^CG[CT]C
AfaI          207  GTAC                 GT^AC
AlwNI         384  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           43  GGGCCC               GGGCC^C
ApoI          434  AAATTC               [GA]^AATT[CT]
Asp700I       420  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         447  GAGCAC               G[AT]GC[AT]^C
BbeI          480  GGCGCC               GGCGC^C
BbsI          693  GAAGAC               GAAGAC(2/6)
BfaI          103  CTAG                 C^TAG
BpuAI         693  GAAGAC               GAAGAC(2/6)
BsaHI         480  GGCGCC               G[GA]^CG[CT]C
BsaI          440  GAGACC               GGTCTC(1/5)
BsiEI         612  CGACCG               CG[GA][CT]^CG
BsiHKAI       447  GAGCAC               G[AT]GC[AT]^C
BsmAI         440  GAGAC                GTCTC(1/5)
Bsp1407I      206  TGTACA               T^GTACA
BspMI         407  GCAGGT               ACCTGC(4/8)
BsrBI         547  GAGCGG               CCGCTC(-3/-3)
BsrGI         206  TGTACA               T^GTACA
BsrI          452  CCAGT                ACTGG(1/-1)
BssHII        106  GCGCGC               G^CGCGC
BstEII        361  GGTGACC              G^GT[ACGT]ACC
BstPI         361  GGTGACC              G^GT[ACGT]ACC
BstUI         107  CGCG                 CG^CG
DpnII         315  GATC                 ^GATC
DsaI          122  CCGTGG               C^C[GA][CT]GG
EcoO65I       361  GGTGACC              G^GT[ACGT]ACC
Hin1I         480  GGCGCC               G[GA]^CG[CT]C
HindIII       706  AAGCTT               A^AGCTT
KasI          480  GGCGCC               G^GCGCC
MaeI          103  CTAG                 C^TAG
MboI          315  GATC                 ^GATC
MboII         693  GAAGA                GAAGA(8/7)
MslI          273  CACACTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        751  CAGCTG               C[AC]G^C[GT]G
MvnI          107  CGCG                 CG^CG
NarI          480  GGCGCC               GG^CGCC
NdeII         315  GATC                 ^GATC
NheI          102  GCTAGC               G^CTAGC
PflMI         747  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         581  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         751  CAGCTG               CAG^CTG
RsaI          207  GTAC                 GT^AC
Sau3AI        315  GATC                 ^GATC
SfoI          480  GGCGCC               GGC^GCC
SspBI         206  TGTACA               T^GTACA
Tsp509I       435  AATT                 ^AATT
Van91I        747  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          170  CCACTTGCGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          420  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AvaII         582  GGACC                G^G[AT]CC
              687  GGACC               
BanI          410  GGTGCC               G^G[CT][GA]CC
              480  GGCGCC              
BanII          43  GGGCCC               G[GA]GC[CT]^C
              513  GGGCTC              
BpmI          544  CTGGAG               CTGGAG(16/14)
              721  CTGGAG              
BsaWI         443  ACCGGA               [AT]^CCGG[AT]
              689  ACCGGA              
BseRI          94  GAGGAG               GAGGAG(10/8)
              133  GAGGAG              
BsgI          268  CTGCAC               GTGCAG(16/14)
              532  CTGCAC              
BssSI         577  CACGAG               CACGAG(-5/-1)
               16  CTCGTG              
DraII         581  AGGACCT              [GA]G^G[ACGT]CC[CT]
              716  GGGGCCT             
EcoO109I      581  AGGACCT              [GA]G^G[ACGT]CC[CT]
              716  GGGGCCT             
HaeII         480  GGCGCC               [GA]GCGC^[CT]
              540  GGCGCT              
MaeIII        362  GTGAC                ^GT[ACGT]AC
              644  GTCAC               
NlaIII        300  CATG                 CATG^
              400  CATG                
PstI          337  CTGCAG               CTGCA^G
              415  CTGCAG              
SmaI           46  CCCGGG               CCC^GGG
              485  CCCGGG              
Tsp45I        362  GTGAC                ^GT[GC]AC
              644  GTCAC               
XmaCI          46  CCCGGG               C^CCGGG
              485  CCCGGG              
XmaI           46  CCCGGG               C^CCGGG
              485  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AvaI        BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsoBI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MnlI        
MspI        MvaI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        TspRI       TthHB8I     
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