Restriction map for pF1KB5337
 (Linear) (Six-base) MAPSORT of: KIBB5337.seq  Check: 2392  from: 1  to: 774



KIBB5337.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     398     774 
   Size:        398     376
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     774 
   Size:        366     408
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     430     774 
   Size:        430     344
 
AlwNI CAG_nnn'CTG

Cuts at:      0     389     774 
   Size:        389     385
 
ApaI G_GGCC'C

Cuts at:      0      47     774 
   Size:         47     727
 
ApoI r'AATT_y

Cuts at:      0     434     774 
   Size:        434     340
 
AvaI C'yCGr_G

Cuts at:      0      46     225     248     485     774 
   Size:         46     179      23     237     289

  Fragments arranged by size: 

                 289     237     179      46      23
 
BanI G'GyrC_C

Cuts at:      0     410     480     774 
   Size:        410      70     294

  Fragments arranged by size: 

                 410     294      70
 
BanII G_rGCy'C

Cuts at:      0      47     517     774 
   Size:         47     470     257

  Fragments arranged by size: 

                 470     257      47
 
BbeI G_GCGC'C

Cuts at:      0     484     774 
   Size:        484     290
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     700     774 
   Size:        700      74
 
Bme1580I G_kGCm'C

Cuts at:      0      47     399     509     774 
   Size:         47     352     110     265

  Fragments arranged by size: 

                 352     265     110      47
 
BmtI G_CTAG'C

Cuts at:      0     106     774 
   Size:        106     668
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     742     774 
   Size:        565     177      32

  Fragments arranged by size: 

                 565     177      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     434     774 
   Size:        434     340
 
BsaHI Gr'CG_yC

Cuts at:      0     481     774 
   Size:        481     293
 
BsaWI w'CCGG_w

Cuts at:      0     443     689     774 
   Size:        443     246      85

  Fragments arranged by size: 

                 443     246      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     438     468     774 
   Size:        438      30     306

  Fragments arranged by size: 

                 438     306      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     148     774 
   Size:        109      39     626

  Fragments arranged by size: 

                 626     109      39
 
BseYI C'CCAG_C

Cuts at:      0     648     774 
   Size:        648     126
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     517     774 
   Size:        253     264     257

  Fragments arranged by size: 

                 264     257     253
 
BsiEI CG_ry'CG

Cuts at:      0     615     774 
   Size:        615     159
 
BsiHKAI G_wGCw'C

Cuts at:      0     451     774 
   Size:        451     323
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     399     451     509     517     774 
   Size:         47     352      52      58       8     257

  Fragments arranged by size: 

                 352     257      58      52      47       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     398     774 
   Size:        398     376
 
BsrBI CCG'CTC

Cuts at:      0     549     774 
   Size:        549     225
 
BsrGI T'GTAC_A

Cuts at:      0     206     774 
   Size:        206     568
 
BssHII G'CGCG_C

Cuts at:      0     106     774 
   Size:        106     668
 
BssSI C'ACGA_G

Cuts at:      0      16     577     774 
   Size:         16     561     197

  Fragments arranged by size: 

                 561     197      16
 
BstEII G'GTnAC_C

Cuts at:      0     361     774 
   Size:        361     413
 
BtgI C'CryG_G

Cuts at:      0     122     774 
   Size:        122     652
 
BtsI GCAGTG_nn'

Cuts at:      0      75     646     774 
   Size:         75     571     128

  Fragments arranged by size: 

                 571     128      75
 
EaeI y'GGCC_r

Cuts at:      0      20      35     554     774 
   Size:         20      15     519     220

  Fragments arranged by size: 

                 519     220      20      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     325     774 
   Size:        325     449
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     565     742     774 
   Size:        366     199     177      32

  Fragments arranged by size: 

                 366     199     177      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     582     717     774 
   Size:        582     135      57

  Fragments arranged by size: 

                 582     135      57
 
HaeII r_GCGC'y

Cuts at:      0     484     544     774 
   Size:        484      60     230

  Fragments arranged by size: 

                 484     230      60
 
HindIII A'AGCT_T

Cuts at:      0     706     774 
   Size:        706      68
 
KasI G'GCGC_C

Cuts at:      0     480     774 
   Size:        480     294
 
MslI CAynn'nnrTG

Cuts at:      0     277     774 
   Size:        277     497
 
MspA1I CmG'CkG

Cuts at:      0     753     774 
   Size:        753      21
 
NarI GG'CG_CC

Cuts at:      0     481     774 
   Size:        481     293
 
NheI G'CTAG_C

Cuts at:      0     102     774 
   Size:        102     672
 
PasI CC'CwG_GG

Cuts at:      0     180     510     774 
   Size:        180     330     264

  Fragments arranged by size: 

                 330     264     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     753     774 
   Size:        753      21
 
PfoI T'CCnGG_A

Cuts at:      0     326     774 
   Size:        326     448
 
PpuMI rG'GwC_Cy

Cuts at:      0     582     774 
   Size:        582     192
 
PspOMI G'GGCC_C

Cuts at:      0      43     774 
   Size:         43     731
 
PstI C_TGCA'G

Cuts at:      0     341     419     774 
   Size:        341      78     355

  Fragments arranged by size: 

                 355     341      78
 
PvuII CAG'CTG

Cuts at:      0     753     774 
   Size:        753      21
 
SfcI C'TryA_G

Cuts at:      0     337     390     415     774 
   Size:        337      53      25     359

  Fragments arranged by size: 

                 359     337      53      25
 
SfoI GGC'GCC

Cuts at:      0     482     774 
   Size:        482     292
 
SmaI CCC'GGG

Cuts at:      0      48     487     774 
   Size:         48     439     287

  Fragments arranged by size: 

                 439     287      48
 
SrfI GCCC'GGGC

Cuts at:      0      48     774 
   Size:         48     726
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     589     662     774 
   Size:        589      73     112

  Fragments arranged by size: 

                 589     112      73
 
TatI w'GTAC_w

Cuts at:      0     206     774 
   Size:        206     568
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     438     470     774 
   Size:        438      32     304

  Fragments arranged by size: 

                 438     304      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     177     774 
   Size:        177     597
 
XmaI C'CCGG_G

Cuts at:      0      46     485     774 
   Size:         46     439     289

  Fragments arranged by size: 

                 439     289      46
 
XmnI GAAnn'nnTTC

Cuts at:      0     424     774 
   Size:        424     350
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI Bme1580I     BmtI     BpmI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstEII     BtgI     BtsI
     EaeI     EciI  Eco57MI EcoO109I    HaeII  HindIII     KasI     MslI
   MspA1I     NarI     NheI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SfcI     SfoI     SmaI     SrfI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XhoI     ZraI
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