Restriction map for pF1KA0913
 (Linear) (Six-base) MAPSORT of: KIAA0913.seq  Check: 8447  from: 1  to: 5388



KIAA0913.orf

 With 182 enzymes: SgfI * 

                        August 18, 2008 14:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     237    1786    4174    4234    4451    4874    5138    5388 
   Size:        237    1549    2388      60     217     423     264     250

  Fragments arranged by size: 

                2388    1549     423     264     250     237     217      60
            
 
Acc65I G'GTAC_C

Cuts at:      0     193    5388 
   Size:        193    5195
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1378    1724    2203    4151    5388 
   Size:        921     457     346     479    1948    1237

  Fragments arranged by size: 

                1948    1237     921     479     457     346
 
AfeI AGC'GCT

Cuts at:      0      37    5164    5388 
   Size:         37    5127     224

  Fragments arranged by size: 

                5127     224      37
 
AflII C'TTAA_G

Cuts at:      0    2911    3375    5388 
   Size:       2911     464    2013

  Fragments arranged by size: 

                2911    2013     464
 
AflIII A'CryG_T

Cuts at:      0    2845    5388 
   Size:       2845    2543
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4832    5388 
   Size:       4832     556
 
AleI CACnn'nnGTG

Cuts at:      0     467    1187    4342    4621    4702    5132    5388 
   Size:        467     720    3155     279      81     430     256

  Fragments arranged by size: 

                3155     720     467     430     279     256      81
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     719    5388 
   Size:        719    4669
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2347    2379    5388 
   Size:       2347      32    3009

  Fragments arranged by size: 

                3009    2347      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     985    1954    2059    2298    2785    2970    3079 
   Size:        301     684     969     105     239     487     185     109

Cuts at:   3079    3088    3115    4294    5103    5388 
   Size:          9      27    1179     809     285

  Fragments arranged by size: 

                1179     969     809     684     487     301     285     239
                 185     109     105      27       9
 
ApaI G_GGCC'C

Cuts at:      0     152     836    1527    1533    3040    3057    3408    4041 
   Size:        152     684     691       6    1507      17     351     633

Cuts at:   4041    4288    5388 
   Size:        247    1100

  Fragments arranged by size: 

                1507    1100     691     684     633     351     247     152
                  17       6
 
ApaLI G'TGCA_C

Cuts at:      0    5020    5388 
   Size:       5020     368
 
AvaI C'yCGr_G

Cuts at:      0    1526    1580    3521    3565    4377    4816    5365    5388 
   Size:       1526      54    1941      44     812     439     549      23

  Fragments arranged by size: 

                1941    1526     812     549     439      54      44      23
            
 
AvrII C'CTAG_G

Cuts at:      0    4569    5388 
   Size:       4569     819
 
BanI G'GyrC_C

Cuts at:      0     193     480    1319    1549    2609    3896    4344    4647 
   Size:        193     287     839     230    1060    1287     448     303

Cuts at:   4647    4753    5388 
   Size:        106     635

  Fragments arranged by size: 

                1287    1060     839     635     448     303     287     230
                 193     106
 
BanII G_rGCy'C

Cuts at:      0     152     654     728     816     836    1527    1533    1792 
   Size:        152     502      74      88      20     691       6     259

Cuts at:   1792    1930    3040    3057    3408    3475    3522    4041    4288 
   Size:        138    1110      17     351      67      47     519     247

Cuts at:   4288    4578    5062    5388 
   Size:        290     484     326

  Fragments arranged by size: 

                1110     691     519     502     484     351     326     290
                 259     247     152     138      88      74      67      47
                  20      17       6
 
BbeI G_GCGC'C

Cuts at:      0    1323    5388 
   Size:       1323    4065
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     770     927     956    1402    1835    2806    3181    3535 
   Size:        770     157      29     446     433     971     375     354

Cuts at:   3535    3643    4512    5388 
   Size:        108     869     876

  Fragments arranged by size: 

                 971     876     869     770     446     433     375     354
                 157     108      29
 
BbvCI CC'TCA_GC

Cuts at:      0     731     837    1173    2231    5388 
   Size:        731     106     336    1058    3157

  Fragments arranged by size: 

                3157    1058     731     336     106
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     579     613    2155    2189    3556    3590    3624    3658 
   Size:        579      34    1542      34    1367      34      34      34

Cuts at:   3658    5388 
   Size:       1730

  Fragments arranged by size: 

                1730    1542    1367     579      34      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2373    5388 
   Size:       2373    3015
 
BclI T'GATC_A

Cuts at:      0    2882    3647    5388 
   Size:       2882     765    1741

  Fragments arranged by size: 

                2882    1741     765
 
BfrBI ATG'CAT

Cuts at:      0    4012    5388 
   Size:       4012    1376
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1542    1967    3052    4090    5388 
   Size:       1542     425    1085    1038    1298

  Fragments arranged by size: 

                1542    1298    1085    1038     425
 
BglII A'GATC_T

Cuts at:      0    2918    5388 
   Size:       2918    2470
 
BlpI GC'TnA_GC

Cuts at:      0     752    1051    1791    1819    5056    5388 
   Size:        752     299     740      28    3237     332

  Fragments arranged by size: 

                3237     752     740     332     299      28
 
Bme1580I G_kGCm'C

Cuts at:      0     152     836    1202    1527    1533    3040    3057    3345 
   Size:        152     684     366     325       6    1507      17     288

Cuts at:   3345    3408    3899    4041    4288    4349    4532    4923    5024 
   Size:         63     491     142     247      61     183     391     101

Cuts at:   5024    5139    5259    5388 
   Size:        115     120     129

  Fragments arranged by size: 

                1507     684     491     391     366     325     288     247
                 183     152     142     129     120     115     101      63
                  61      17       6
 
BmgBI CAC'GTC

Cuts at:      0     597    2848    5388 
   Size:        597    2251    2540

  Fragments arranged by size: 

                2540    2251     597
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     649     777    1464    1771    4399    5388 
   Size:        649     128     687     307    2628     989

  Fragments arranged by size: 

                2628     989     687     649     307     128
 
BmtI G_CTAG'C

Cuts at:      0    2305    5388 
   Size:       2305    3083
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      57    5129    5388 
   Size:         57    5072     259

  Fragments arranged by size: 

                5072     259      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1423    1591    1861    2486    2690    3468    4921    5388 
   Size:       1423     168     270     625     204     778    1453     467

  Fragments arranged by size: 

                1453    1423     778     625     467     270     204     168
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     302     731     837    1173    2231    2401    3988    4109 
   Size:        302     429     106     336    1058     170    1587     121

Cuts at:   4109    5388 
   Size:       1279

  Fragments arranged by size: 

                1587    1279    1058     429     336     302     170     121
                 106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2305    2551    4731    5305    5388 
   Size:       2305     246    2180     574      83

  Fragments arranged by size: 

                2305    2180     574     246      83
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3023    3378    5364    5388 
   Size:       3023     355    1986      24

  Fragments arranged by size: 

                3023    1986     355      24
 
BsaAI yAC'GTr

Cuts at:      0    1168    3805    5019    5388 
   Size:       1168    2637    1214     369

  Fragments arranged by size: 

                2637    1214    1168     369
 
BsaHI Gr'CG_yC

Cuts at:      0    1037    1320    5278    5388 
   Size:       1037     283    3958     110

  Fragments arranged by size: 

                3958    1037     283     110
 
BsaWI w'CCGG_w

Cuts at:      0     816    5249    5388 
   Size:        816    4433     139

  Fragments arranged by size: 

                4433     816     139
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25    2203    2233    4930    4960    5388 
   Size:         25    2178      30    2697      30     428

  Fragments arranged by size: 

                2697    2178     428      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82    2086    2173    2773    3465    3530    4589    4834 
   Size:         82    2004      87     600     692      65    1059     245

Cuts at:   4834    4837    5388 
   Size:          3     551

  Fragments arranged by size: 

                2004    1059     692     600     551     245      87      82
                  65       3
 
BseYI C'CCAG_C

Cuts at:      0     574    1313    1520    2119    2152    2176    3115    3401 
   Size:        574     739     207     599      33      24     939     286

Cuts at:   3401    3887    4255    4473    4531    4783    4908    4986    5004 
   Size:        486     368     218      58     252     125      78      18

Cuts at:   5004    5388 
   Size:        384

  Fragments arranged by size: 

                 939     739     599     574     486     384     368     286
                 252     218     207     125      78      58      33      24
                  18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     573     889    1286    3596    3715    3757    4066    4399 
   Size:        573     316     397    2310     119      42     309     333

Cuts at:   4399    4843    5179    5388 
   Size:        444     336     209

  Fragments arranged by size: 

                2310     573     444     397     336     333     316     309
                 209     119      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     728     816    2165    2450    2706    5024    5388 
   Size:        728      88    1349     285     256    2318     364

  Fragments arranged by size: 

                2318    1349     728     364     285     256      88
 
BsiWI C'GTAC_G

Cuts at:      0    1164    5388 
   Size:       1164    4224
 
BsmI GAATG_Cn'

Cuts at:      0     206    2109    3889    4564    4895    5247    5388 
   Size:        206    1903    1780     675     331     352     141

  Fragments arranged by size: 

                1903    1780     675     352     331     206     141
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     763    2278    5083    5388 
   Size:        763    1515    2805     305

  Fragments arranged by size: 

                2805    1515     763     305
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     654     728     816     836    1202    1527    1533 
   Size:        152     502      74      88      20     366     325       6

Cuts at:   1533    1792    1930    2165    2450    2706    3040    3057    3345 
   Size:        259     138     235     285     256     334      17     288

Cuts at:   3345    3408    3475    3522    3899    4041    4288    4349    4532 
   Size:         63      67      47     377     142     247      61     183

Cuts at:   4532    4578    4923    5024    5062    5139    5259    5388 
   Size:         46     345     101      38      77     120     129

  Fragments arranged by size: 

                 502     377     366     345     334     325     288     285
                 259     256     247     235     183     152     142     138
                 129     120     101      88      77      74      67      63
                  61      47      46      38      20      17       6
 
BspEI T'CCGG_A

Cuts at:      0     816    5249    5388 
   Size:        816    4433     139

  Fragments arranged by size: 

                4433     816     139
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     237    1055    1786    3536    3737    4174    4234    4451 
   Size:        237     818     731    1750     201     437      60     217

Cuts at:   4451    4874    5138    5388 
   Size:        423     264     250

  Fragments arranged by size: 

                1750     818     731     437     423     264     250     237
                 217     201      60
 
BsrBI CCG'CTC

Cuts at:      0    1407    1559    4799    4952    5113    5388 
   Size:       1407     152    3240     153     161     275

  Fragments arranged by size: 

                3240    1407     275     161     153     152
 
BsrDI GCAATG_nn'

Cuts at:      0     445    1095    2034    5388 
   Size:        445     650     939    3354

  Fragments arranged by size: 

                3354     939     650     445
 
BsrFI r'CCGG_y

Cuts at:      0    1586    4299    5388 
   Size:       1586    2713    1089

  Fragments arranged by size: 

                2713    1586    1089
 
BssSI C'ACGA_G

Cuts at:      0    2367    5388 
   Size:       2367    3021
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1240    2059    2782    2785    3088    4866    5388 
   Size:       1240     819     723       3     303    1778     522

  Fragments arranged by size: 

                1778    1240     819     723     522     303       3
 
BstEII G'GTnAC_C

Cuts at:      0    2860    5388 
   Size:       2860    2528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1761    2520    4408    4704    4791    5388 
   Size:       1761     759    1888     296      87     597

  Fragments arranged by size: 

                1888    1761     759     597     296      87
 
BstYI r'GATC_y

Cuts at:      0     737    2295    2573    2918    4028    5388 
   Size:        737    1558     278     345    1110    1360

  Fragments arranged by size: 

                1558    1360    1110     737     345     278
 
Bsu36I CC'TnA_GG

Cuts at:      0    3072    3699    3911    4271    5388 
   Size:       3072     627     212     360    1117

  Fragments arranged by size: 

                3072    1117     627     360     212
 
BtgI C'CryG_G

Cuts at:      0     968    3845    4643    5282    5388 
   Size:        968    2877     798     639     106

  Fragments arranged by size: 

                2877     968     798     639     106
 
BtsI GCAGTG_nn'

Cuts at:      0     192     393     901    1194    1354    1782    2265    3345 
   Size:        192     201     508     293     160     428     483    1080

Cuts at:   3345    4054    4100    4328    4424    4616    4690    4722    4759 
   Size:        709      46     228      96     192      74      32      37

Cuts at:   4759    5388 
   Size:        629

  Fragments arranged by size: 

                1080     709     629     508     483     428     293     228
                 201     192     192     160      96      74      46      37
                  32
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     230     265     897     932    5388 
   Size:        230      35     632      35    4456

  Fragments arranged by size: 

                4456     632     230      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     600    1277    1765    2644    4552    5388 
   Size:        600     677     488     879    1908     836

  Fragments arranged by size: 

                1908     879     836     677     600     488
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1267    5388 
   Size:       1267    4121
 
EaeI y'GGCC_r

Cuts at:      0     377    1286    1426    1543    1853    2675    3323    4398 
   Size:        377     909     140     117     310     822     648    1075

Cuts at:   4398    5372    5388 
   Size:        974      16

  Fragments arranged by size: 

                1075     974     909     822     648     377     310     140
                 117      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     344     424     619    1417    1454    2126    3459    4094 
   Size:        344      80     195     798      37     672    1333     635

Cuts at:   4094    5388 
   Size:       1294

  Fragments arranged by size: 

                1333    1294     798     672     635     344     195      80
                  37
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     191    1817    5388 
   Size:        191    1626    3571

  Fragments arranged by size: 

                3571    1626     191
 
EcoICRI GAG'CTC

Cuts at:      0     726     814    5388 
   Size:        726      88    4574

  Fragments arranged by size: 

                4574     726      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1378    1423    1591    1724    1861    2203    2486 
   Size:        921     457      45     168     133     137     342     283

Cuts at:   2486    2690    3468    4151    4921    5388 
   Size:        204     778     683     770     467

  Fragments arranged by size: 

                 921     778     770     683     467     457     342     283
                 204     168     137     133      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     503    2404    5388 
   Size:        503    1901    2984

  Fragments arranged by size: 

                2984    1901     503
 
EcoO109I rG'GnC_Cy

Cuts at:      0     148     441     509     832     833    1530    1566    1665 
   Size:        148     293      68     323       1     697      36      99

Cuts at:   1665    1684    2235    3036    3053    3054    3085    3124    3287 
   Size:         19     551     801      17       1      31      39     163

Cuts at:   3287    3405    3715    4038    4284    5388 
   Size:        118     310     323     246    1104

  Fragments arranged by size: 

                1104     801     697     551     323     323     310     293
                 246     163     148     118      99      68      39      36
                  31      19      17       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2430    2937    5388 
   Size:       2430     507    2451

  Fragments arranged by size: 

                2451    2430     507
 
FspI TGC'GCA

Cuts at:      0    2246    5388 
   Size:       2246    3142
 
FspAI rTGC'GCAy

Cuts at:      0    2246    5388 
   Size:       2246    3142
 
HaeII r_GCGC'y

Cuts at:      0      39    1323    5166    5267    5388 
   Size:         39    1284    3843     101     121

  Fragments arranged by size: 

                3843    1284     121     101      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     203     235     483     515     641     655     673     687 
   Size:        203      32     248      32     126      14      18      14

Cuts at:    687     934     966    3904    3922    3936    3954    4818    4850 
   Size:        247      32    2938      18      14      18     864      32

Cuts at:   4850    4896    4928    5388 
   Size:         46      32     460

  Fragments arranged by size: 

                2938     864     460     248     247     203     126      46
                  32      32      32      32      32      18      18      18
                  14      14      14
 
HincII GTy'rAC

Cuts at:      0     788    2423    5388 
   Size:        788    1635    2965

  Fragments arranged by size: 

                2965    1635     788
 
HindIII A'AGCT_T

Cuts at:      0    2908    5388 
   Size:       2908    2480
 
KasI G'GCGC_C

Cuts at:      0    1319    5388 
   Size:       1319    4069
 
KpnI G_GTAC'C

Cuts at:      0     197    5388 
   Size:        197    5191
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1085    2046    4055    4866    5388 
   Size:       1085     961    2009     811     522

  Fragments arranged by size: 

                2009    1085     961     811     522
 
MscI TGG'CCA

Cuts at:      0     379    2677    3325    4400    5374    5388 
   Size:        379    2298     648    1075     974      14

  Fragments arranged by size: 

                2298    1075     974     648     379      14
 
MslI CAynn'nnrTG

Cuts at:      0     467    1187    1244    2246    3319    4007    4093    4342 
   Size:        467     720      57    1002    1073     688      86     249

Cuts at:   4342    4357    4621    4702    4868    5132    5287    5388 
   Size:         15     264      81     166     264     155     101

  Fragments arranged by size: 

                1073    1002     720     688     467     264     264     249
                 166     155     101      86      81      57      15
 
MspA1I CmG'CkG

Cuts at:      0     145     539     560     693     985    1317    1341    1432 
   Size:        145     394      21     133     292     332      24      91

Cuts at:   1432    1493    1615    1680    1702    3610    4035    4315    5100 
   Size:         61     122      65      22    1908     425     280     785

Cuts at:   5100    5355    5388 
   Size:        255      33

  Fragments arranged by size: 

                1908     785     425     394     332     292     280     255
                 145     133     122      91      65      61      33      24
                  22      21
 
NarI GG'CG_CC

Cuts at:      0    1320    5388 
   Size:       1320    4068
 
NcoI C'CATG_G

Cuts at:      0    3845    4643    5282    5388 
   Size:       3845     798     639     106

  Fragments arranged by size: 

                3845     798     639     106
 
NheI G'CTAG_C

Cuts at:      0    2301    5388 
   Size:       2301    3087
 
NsiI A_TGCA'T

Cuts at:      0    4014    5388 
   Size:       4014    1374
 
NspI r_CATG'y

Cuts at:      0     429    1001    1257    2245    2478    4506    4873    5184 
   Size:        429     572     256     988     233    2028     367     311

Cuts at:   5184    5388 
   Size:        204

  Fragments arranged by size: 

                2028     988     572     429     367     311     256     233
                 204
 
PasI CC'CwG_GG

Cuts at:      0    1887    3049    3468    4288    5388 
   Size:       1887    1162     419     820    1100

  Fragments arranged by size: 

                1887    1162    1100     820     419
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     600    1477    2457    3416    3846    5154    5388 
   Size:        600     877     980     959     430    1308     234

  Fragments arranged by size: 

                1308     980     959     877     600     430     234
 
PfoI T'CCnGG_A

Cuts at:      0     761    3826    4383    5388 
   Size:        761    3065     557    1005

  Fragments arranged by size: 

                3065    1005     761     557
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2813    2845    5388 
   Size:       2813      32    2543

  Fragments arranged by size: 

                2813    2543      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     441    1665    1684    3287    3715    5388 
   Size:        441    1224      19    1603     428    1673

  Fragments arranged by size: 

                1673    1603    1224     441     428      19
 
PspOMI G'GGCC_C

Cuts at:      0     148     832    1523    1529    3036    3053    3404    4037 
   Size:        148     684     691       6    1507      17     351     633

Cuts at:   4037    4284    5388 
   Size:        247    1104

  Fragments arranged by size: 

                1507    1104     691     684     633     351     247     148
                  17       6
 
PstI C_TGCA'G

Cuts at:      0     559     639    2064    3084    4185    5075    5099    5388 
   Size:        559      80    1425    1020    1101     890      24     289

  Fragments arranged by size: 

                1425    1101    1020     890     559     289      80      24
            
 
PvuII CAG'CTG

Cuts at:      0     560     693    1341    1615    1702    3610    5388 
   Size:        560     133     648     274      87    1908    1778

  Fragments arranged by size: 

                1908    1778     648     560     274     133      87
 
RsrII CG'GwC_CG

Cuts at:      0      57    5388 
   Size:         57    5331
 
SacI G_AGCT'C

Cuts at:      0     728     816    5388 
   Size:        728      88    4572

  Fragments arranged by size: 

                4572     728      88
 
SanDI GG'GwC_CC

Cuts at:      0    1665    1684    3287    5388 
   Size:       1665      19    1603    2101

  Fragments arranged by size: 

                2101    1665    1603      19
 
SapI GCTCTTCn'nnn_

Cuts at:      0     424    1417    3459    5388 
   Size:        424     993    2042    1929

  Fragments arranged by size: 

                2042    1929     993     424
 
ScaI AGT'ACT

Cuts at:      0     855    5388 
   Size:        855    4533
 
SfcI C'TryA_G

Cuts at:      0     310     473     555     635     679    1488    2012    2060 
   Size:        310     163      82      80      44     809     524      48

Cuts at:   2060    2253    2491    3080    4181    4221    4432    4449    5071 
   Size:        193     238     589    1101      40     211      17     622

Cuts at:   5071    5095    5311    5388 
   Size:         24     216      77

  Fragments arranged by size: 

                1101     809     622     589     524     310     238     216
                 211     193     163      82      80      77      48      44
                  40      24      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1967    5388 
   Size:       1967    3421
 
SfoI GGC'GCC

Cuts at:      0    1321    5388 
   Size:       1321    4067
 
SmaI CCC'GGG

Cuts at:      0    1528    1582    3523    4379    5388 
   Size:       1528      54    1941     856    1009

  Fragments arranged by size: 

                1941    1528    1009     856      54
 
SmlI C'TyrA_G

Cuts at:      0    2320    2566    2911    3375    4710    5320    5365    5388 
   Size:       2320     246     345     464    1335     610      45      23

  Fragments arranged by size: 

                2320    1335     610     464     345     246      45      23
            
 
SnaBI TAC'GTA

Cuts at:      0    1168    5388 
   Size:       1168    4220
 
SphI G_CATG'C

Cuts at:      0     429    2245    4873    5388 
   Size:        429    1816    2628     515

  Fragments arranged by size: 

                2628    1816     515     429
 
SrfI GCCC'GGGC

Cuts at:      0    1528    3523    5388 
   Size:       1528    1995    1865

  Fragments arranged by size: 

                1995    1865    1528
 
StuI AGG'CCT

Cuts at:      0    1441    1465    2266    2499    2518    4084    5388 
   Size:       1441      24     801     233      19    1566    1304

  Fragments arranged by size: 

                1566    1441    1304     801     233      24      19
 
StyI C'CwwG_G

Cuts at:      0    1625    1754    2342    2483    2513    2519    2789    3214 
   Size:       1625     129     588     141      30       6     270     425

Cuts at:   3214    3765    3845    4569    4643    4749    5045    5282    5339 
   Size:        551      80     724      74     106     296     237      57

Cuts at:   5339    5388 
   Size:         49

  Fragments arranged by size: 

                1625     724     588     551     425     296     270     237
                 141     129     106      80      74      57      49      30
                   6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     607    1645    1753    2373    2954    4487    4665    5388 
   Size:        607    1038     108     620     581    1533     178     723

  Fragments arranged by size: 

                1533    1038     723     620     607     581     178     108
            
 
TatI w'GTAC_w

Cuts at:      0     853    4659    5186    5388 
   Size:        853    3806     527     202

  Fragments arranged by size: 

                3806     853     527     202
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2628    2660    4647    4679    5388 
   Size:       2628      32    1987      32     709

  Fragments arranged by size: 

                2628    1987     709      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1037    2116    2729    5388 
   Size:       1037    1079     613    2659

  Fragments arranged by size: 

                2659    1079    1037     613
 
XhoI C'TCGA_G

Cuts at:      0    5365    5388 
   Size:       5365      23
 
XmaI C'CCGG_G

Cuts at:      0    1526    1580    3521    4377    5388 
   Size:       1526      54    1941     856    1011

  Fragments arranged by size: 

                1941    1526    1011     856      54
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI    AvrII     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NheI     NsiI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI
    PvuII    RsrII     SacI    SanDI     SapI     ScaI     SfcI     SfiI
     SfoI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AgeI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI    BsaBI    BsiEI    BspHI    BsrGI   BssHII    BstBI
  BstZ17I    BtgZI     ClaI     DraI     EagI    EcoRI    EcoRV     FseI
     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI     NruI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PvuI    SacII     SalI     SbfI    SexAI     SgfI    SgrAI     SpeI
     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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