Restriction map for pF1KA0627
 (Linear) (Six-base) MAPSORT of: KIAA0627.seq  Check: 4704  from: 1  to: 3882



KIAA0627.orf

 With 182 enzymes: SgfI * 

                        August 5, 2008 16:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     133    1123    1180    3882 
   Size:        133     990      57    2702

  Fragments arranged by size: 

                2702     990     133      57
 
AccI GT'mk_AC

Cuts at:      0    1018    1740    3882 
   Size:       1018     722    2142

  Fragments arranged by size: 

                2142    1018     722
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     545    1280    2299    2822    2855    3882 
   Size:        358     187     735    1019     523      33    1027

  Fragments arranged by size: 

                1027    1019     735     523     358     187      33
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3559    3882 
   Size:       3559     323
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     120     152    3882 
   Size:        120      32    3730

  Fragments arranged by size: 

                3730     120      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1258    1786    3841    3882 
   Size:       1258     528    2055      41

  Fragments arranged by size: 

                2055    1258     528      41
 
ApaLI G'TGCA_C

Cuts at:      0    3827    3882 
   Size:       3827      55
 
ApoI r'AATT_y

Cuts at:      0     290     344     700     785    2090    2439    2847    3882 
   Size:        290      54     356      85    1305     349     408    1035

  Fragments arranged by size: 

                1305    1035     408     356     349     290      85      54
            
 
AseI AT'TA_AT

Cuts at:      0    3581    3882 
   Size:       3581     301
 
AvaI C'yCGr_G

Cuts at:      0    2468    3047    3882 
   Size:       2468     579     835

  Fragments arranged by size: 

                2468     835     579
 
AvrII C'CTAG_G

Cuts at:      0    2022    3882 
   Size:       2022    1860
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     611     644    3882 
   Size:        611      33    3238

  Fragments arranged by size: 

                3238     611      33
 
BamHI G'GATC_C

Cuts at:      0    2421    3882 
   Size:       2421    1461
 
BanI G'GyrC_C

Cuts at:      0    1135    1192    1675    3882 
   Size:       1135      57     483    2207

  Fragments arranged by size: 

                2207    1135     483      57
 
BanII G_rGCy'C

Cuts at:      0    1432    3882 
   Size:       1432    2450
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     901    1742    3714    3882 
   Size:        901     841    1972     168

  Fragments arranged by size: 

                1972     901     841     168
 
BbvCI CC'TCA_GC

Cuts at:      0     803    1464    3488    3882 
   Size:        803     661    2024     394

  Fragments arranged by size: 

                2024     803     661     394
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1221    3882 
   Size:       1221    2661
 
BclI T'GATC_A

Cuts at:      0     513    2346    3882 
   Size:        513    1833    1536

  Fragments arranged by size: 

                1833    1536     513
 
BfrBI ATG'CAT

Cuts at:      0    1857    1885    3882 
   Size:       1857      28    1997

  Fragments arranged by size: 

                1997    1857      28
 
BglII A'GATC_T

Cuts at:      0    3481    3882 
   Size:       3481     401
 
Bme1580I G_kGCm'C

Cuts at:      0    1197    1680    3831    3882 
   Size:       1197     483    2151      51

  Fragments arranged by size: 

                2151    1197     483      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     324    3882 
   Size:        324    3558
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     386    1373    1635    3863    3882 
   Size:        206     180     987     262    2228      19

  Fragments arranged by size: 

                2228     987     262     206     180      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     434     803    1182    1464    3488    3882 
   Size:        434     369     379     282    2024     394

  Fragments arranged by size: 

                2024     434     394     379     369     282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    2961    3091    3882 
   Size:        907    2054     130     791

  Fragments arranged by size: 

                2054     907     791     130
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1391    3882 
   Size:       1391    2491
 
BsaBI GATnn'nnATC

Cuts at:      0    1842    3882 
   Size:       1842    2040
 
BsaHI Gr'CG_yC

Cuts at:      0    1871    3882 
   Size:       1871    2011
 
BsaWI w'CCGG_w

Cuts at:      0     103    3882 
   Size:        103    3779
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2745    2775    3882 
   Size:       2745      30    1107

  Fragments arranged by size: 

                2745    1107      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     699    1810    3024    3860    3882 
   Size:        699    1111    1214     836      22

  Fragments arranged by size: 

                1214    1111     836     699      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     356     614    1208    3882 
   Size:         74     282     258     594    2674

  Fragments arranged by size: 

                2674     594     282     258      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     420    1432    3831    3882 
   Size:        420    1012    2399      51

  Fragments arranged by size: 

                2399    1012     420      51
 
BsmI GAATG_Cn'

Cuts at:      0    1128    1206    1857    1859    3882 
   Size:       1128      78     651       2    2023

  Fragments arranged by size: 

                2023    1128     651      78       2
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     681    3882 
   Size:        681    3201
 
Bsp1286I G_dGCh'C

Cuts at:      0     420    1197    1432    1680    3831    3882 
   Size:        420     777     235     248    2151      51

  Fragments arranged by size: 

                2151     777     420     248     235      51
 
BspEI T'CCGG_A

Cuts at:      0     103    3882 
   Size:        103    3779
 
BspHI T'CATG_A

Cuts at:      0     789    3434    3882 
   Size:        789    2645     448

  Fragments arranged by size: 

                2645     789     448
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     133    1123    1180    1195    3653    3882 
   Size:        133     990      57      15    2458     229

  Fragments arranged by size: 

                2458     990     229     133      57      15
 
BsrDI GCAATG_nn'

Cuts at:      0    2655    3007    3882 
   Size:       2655     352     875

  Fragments arranged by size: 

                2655     875     352
 
BsrFI r'CCGG_y

Cuts at:      0    1376    3882 
   Size:       1376    2506
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1150    1297    1891    3882 
   Size:       1150     147     594    1991

  Fragments arranged by size: 

                1991    1150     594     147
 
BstBI TT'CG_AA

Cuts at:      0    2637    3882 
   Size:       2637    1245
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     856    2418    2711    3882 
   Size:        856    1562     293    1171

  Fragments arranged by size: 

                1562    1171     856     293
 
BstYI r'GATC_y

Cuts at:      0     438     445    2251    2421    3459    3481    3882 
   Size:        438       7    1806     170    1038      22     401

  Fragments arranged by size: 

                1806    1038     438     401     170      22       7
 
Bsu36I CC'TnA_GG

Cuts at:      0     152    1707    3388    3882 
   Size:        152    1555    1681     494

  Fragments arranged by size: 

                1681    1555     494     152
 
BtgI C'CryG_G

Cuts at:      0    1489    2666    3882 
   Size:       1489    1177    1216

  Fragments arranged by size: 

                1489    1216    1177
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1895    3882 
   Size:       1895    1987
 
BtsI GCAGTG_nn'

Cuts at:      0     352     942    2468    3538    3708    3882 
   Size:        352     590    1526    1070     170     174

  Fragments arranged by size: 

                1526    1070     590     352     174     170
 
DraI TTT'AAA

Cuts at:      0    3411    3882 
   Size:       3411     471
 
DraIII CAC_nnn'GTG

Cuts at:      0    1108    3882 
   Size:       1108    2774
 
EaeI y'GGCC_r

Cuts at:      0    1266    2408    3509    3882 
   Size:       1266    1142    1101     373

  Fragments arranged by size: 

                1266    1142    1101     373
 
EarI CTCTTCn'nnn_

Cuts at:      0     898     953    1041    1389    3882 
   Size:        898      55      88     348    2493

  Fragments arranged by size: 

                2493     898     348      88      55
 
EcoICRI GAG'CTC

Cuts at:      0    1430    3882 
   Size:       1430    2452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     358     386     545    1280    1373    1635    2299 
   Size:        206     152      28     159     735      93     262     664

Cuts at:   2299    2822    2855    3863    3882 
   Size:        523      33    1008      19

  Fragments arranged by size: 

                1008     735     664     523     262     206     159     152
                  93      33      28      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0     965    1703    3882 
   Size:        965     738    2179

  Fragments arranged by size: 

                2179     965     738
 
EcoRI G'AATT_C

Cuts at:      0     785    3882 
   Size:        785    3097
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2203    2315    3882 
   Size:       2203     112    1567

  Fragments arranged by size: 

                2203    1567     112
 
HaeII r_GCGC'y

Cuts at:      0     524    1182    3882 
   Size:        524     658    2700

  Fragments arranged by size: 

                2700     658     524
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     430     462     952     984    1436    1468 
   Size:         22      32     376      32     490      32     452      32

Cuts at:   1468    3610    3642    3882 
   Size:       2142      32     240

  Fragments arranged by size: 

                2142     490     452     376     240      32      32      32
                  32      32      22
 
HincII GTy'rAC

Cuts at:      0    1741    3781    3882 
   Size:       1741    2040     101

  Fragments arranged by size: 

                2040    1741     101
 
HindIII A'AGCT_T

Cuts at:      0      21     215     464    2364    2617    3002    3713    3882 
   Size:         21     194     249    1900     253     385     711     169

  Fragments arranged by size: 

                1900     711     385     253     249     194     169      21
            
 
MfeI C'AATT_G

Cuts at:      0     657    1995    3882 
   Size:        657    1338    1887

  Fragments arranged by size: 

                1887    1338     657
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     389    3882 
   Size:        138     251    3493

  Fragments arranged by size: 

                3493     251     138
 
MscI TGG'CCA

Cuts at:      0    2410    3511    3882 
   Size:       2410    1101     371

  Fragments arranged by size: 

                2410    1101     371
 
MslI CAynn'nnrTG

Cuts at:      0     623    1117    1862    2101    2180    3541    3882 
   Size:        623     494     745     239      79    1361     341

  Fragments arranged by size: 

                1361     745     623     494     341     239      79
 
MspA1I CmG'CkG

Cuts at:      0      67    3882 
   Size:         67    3815
 
NcoI C'CATG_G

Cuts at:      0    2666    3882 
   Size:       2666    1216
 
NdeI CA'TA_TG

Cuts at:      0    1848    3882 
   Size:       1848    2034
 
NsiI A_TGCA'T

Cuts at:      0    1859    1887    3882 
   Size:       1859      28    1995

  Fragments arranged by size: 

                1995    1859      28
 
NspI r_CATG'y

Cuts at:      0     754    2142    3882 
   Size:        754    1388    1740

  Fragments arranged by size: 

                1740    1388     754
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1300    1720    3882 
   Size:       1300     420    2162

  Fragments arranged by size: 

                2162    1300     420
 
PfoI T'CCnGG_A

Cuts at:      0    1083    1389    2424    3882 
   Size:       1083     306    1035    1458

  Fragments arranged by size: 

                1458    1083    1035     306
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     675     707    3882 
   Size:        675      32    3175

  Fragments arranged by size: 

                3175     675      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     965    3882 
   Size:        965    2917
 
PshAI GACnn'nnGTC

Cuts at:      0    1619    1746    2978    3882 
   Size:       1619     127    1232     904

  Fragments arranged by size: 

                1619    1232     904     127
 
PsiI TTA'TAA

Cuts at:      0     211    2437    3882 
   Size:        211    2226    1445

  Fragments arranged by size: 

                2226    1445     211
 
PstI C_TGCA'G

Cuts at:      0    1097    1134    2033    3570    3882 
   Size:       1097      37     899    1537     312

  Fragments arranged by size: 

                1537    1097     899     312      37
 
PvuII CAG'CTG

Cuts at:      0      67    3882 
   Size:         67    3815
 
SacI G_AGCT'C

Cuts at:      0    1432    3882 
   Size:       1432    2450
 
SalI G'TCGA_C

Cuts at:      0    1739    3882 
   Size:       1739    2143
 
SfcI C'TryA_G

Cuts at:      0     476    1019    1093    1130    1503    1911    2029    3566 
   Size:        476     543      74      37     373     408     118    1537

Cuts at:   3566    3882 
   Size:        316

  Fragments arranged by size: 

                1537     543     476     408     373     316     118      74
                  37
 
SmlI C'TyrA_G

Cuts at:      0     886    2976    3047    3106    3882 
   Size:        886    2090      71      59     776

  Fragments arranged by size: 

                2090     886     776      71      59
 
SspI AAT'ATT

Cuts at:      0    2325    3308    3882 
   Size:       2325     983     574

  Fragments arranged by size: 

                2325     983     574
 
StuI AGG'CCT

Cuts at:      0    2018    3882 
   Size:       2018    1864
 
StyI C'CwwG_G

Cuts at:      0    1139    1316    2022    2666    3629    3882 
   Size:       1139     177     706     644     963     253

  Fragments arranged by size: 

                1139     963     706     644     253     177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2224    3882 
   Size:       2224    1658
 
TatI w'GTAC_w

Cuts at:      0    2205    3882 
   Size:       2205    1677
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1151    1183    3702    3734    3882 
   Size:       1151      32    2519      32     148

  Fragments arranged by size: 

                2519    1151     148      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1327    3882 
   Size:       1327    2555
 
XhoI C'TCGA_G

Cuts at:      0    3047    3882 
   Size:       3047     835
 
XmnI GAAnn'nnTTC

Cuts at:      0    1857    3882 
   Size:       1857    2025
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI    BciVI     BclI    BfrBI    BglII Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
    PflMI     PfoI     PpiI    PpuMI    PshAI     PsiI     PstI    PvuII
     SacI     SalI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     ApaI     AscI    AsiSI     BbeI     BcgI     BglI     BlpI
    BmgBI     BmtI     BplI    BsaAI    BseYI    BsiEI    BsiWI    BsrBI
    BsrGI   BssHII    BssSI   BstEII  BstZ17I     ClaI    CspCI     DrdI
     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI     HpaI
     KasI     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PmeI     PmlI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XcmI     XmaI     ZraI
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