Restriction map for pF1KB1290
 (Linear) (Six-base) MAPSORT of: KIBB1290.seq  Check: 7873  from: 1  to: 2247



KIBB1290.orf

 With 182 enzymes: SgfI * 

                        August 5, 2008 16:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1257    2247 
   Size:       1257     990
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1580    2247 
   Size:       1580     667
 
AflIII A'CryG_T

Cuts at:      0     183    1551    1974    2247 
   Size:        183    1368     423     273

  Fragments arranged by size: 

                1368     423     273     183
 
AhdI GACnn_n'nnGTC

Cuts at:      0     537    2247 
   Size:        537    1710
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     771    2247 
   Size:        771    1476
 
AlwNI CAG_nnn'CTG

Cuts at:      0     204    2247 
   Size:        204    2043
 
ApaI G_GGCC'C

Cuts at:      0    2198    2247 
   Size:       2198      49
 
ApoI r'AATT_y

Cuts at:      0     621     798    2070    2147    2247 
   Size:        621     177    1272      77     100

  Fragments arranged by size: 

                1272     621     177     100      77
 
AvaI C'yCGr_G

Cuts at:      0     209    1469    1616    2247 
   Size:        209    1260     147     631

  Fragments arranged by size: 

                1260     631     209     147
 
BanI G'GyrC_C

Cuts at:      0     925    1414    2247 
   Size:        925     489     833

  Fragments arranged by size: 

                 925     833     489
 
BanII G_rGCy'C

Cuts at:      0     508     639     844    1187    1896    2143    2198    2247 
   Size:        508     131     205     343     709     247      55      49

  Fragments arranged by size: 

                 709     508     343     247     205     131      55      49
            
 
BclI T'GATC_A

Cuts at:      0    1863    2247 
   Size:       1863     384
 
BglI GCCn_nnn'nGGC

Cuts at:      0      35    1612    2247 
   Size:         35    1577     635

  Fragments arranged by size: 

                1577     635      35
 
BlpI GC'TnA_GC

Cuts at:      0     652    2247 
   Size:        652    1595
 
Bme1580I G_kGCm'C

Cuts at:      0    2198    2247 
   Size:       2198      49
 
BmgBI CAC'GTC

Cuts at:      0     220    2247 
   Size:        220    2027
 
BmtI G_CTAG'C

Cuts at:      0     244    2247 
   Size:        244    2003
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     511    1589    2247 
   Size:        511    1078     658

  Fragments arranged by size: 

                1078     658     511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     611     761    1262    1280    1298    1316    1810 
   Size:         90     521     150     501      18      18      18     494

Cuts at:   1810    1922    2247 
   Size:        112     325

  Fragments arranged by size: 

                 521     501     494     325     150     112      90      18
                  18      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1076    2247 
   Size:       1076    1171
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1828    2247 
   Size:        415    1413     419

  Fragments arranged by size: 

                1413     419     415
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1365    2247 
   Size:       1365     882
 
BsaAI yAC'GTr

Cuts at:      0     184    2247 
   Size:        184    2063
 
BsaHI Gr'CG_yC

Cuts at:      0     691    2247 
   Size:        691    1556
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1926    1956    2247 
   Size:       1926      30     291

  Fragments arranged by size: 

                1926     291      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     364     372     388     412     588    1444    1515 
   Size:        346      18       8      16      24     176     856      71

Cuts at:   1515    1597    1600    1642    1666    1690    1825    2247 
   Size:         82       3      42      24      24     135     422

  Fragments arranged by size: 

                 856     422     346     176     135      82      71      42
                  24      24      24      18      16       8       3
 
BseYI C'CCAG_C

Cuts at:      0    1290    1308    1326    1350    2247 
   Size:       1290      18      18      24     897

  Fragments arranged by size: 

                1290     897      24      18      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     770    1904    2247 
   Size:        542     228    1134     343

  Fragments arranged by size: 

                1134     542     343     228
 
BsiHKAI G_wGCw'C

Cuts at:      0     639    1206    1896    2017    2247 
   Size:        639     567     690     121     230

  Fragments arranged by size: 

                 690     639     567     230     121
 
BsmI GAATG_Cn'

Cuts at:      0    1389    2247 
   Size:       1389     858
 
Bsp1286I G_dGCh'C

Cuts at:      0     508     639     844    1187    1206    1896    2017    2143 
   Size:        508     131     205     343      19     690     121     126

Cuts at:   2143    2198    2247 
   Size:         55      49

  Fragments arranged by size: 

                 690     508     343     205     131     126     121      55
                  49      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1257    2247 
   Size:       1257     990
 
BsrBI CCG'CTC

Cuts at:      0     315     354    1242    1656    1671    2247 
   Size:        315      39     888     414      15     576

  Fragments arranged by size: 

                 888     576     414     315      39      15
 
BsrFI r'CCGG_y

Cuts at:      0     194    2247 
   Size:        194    2053
 
BsrGI T'GTAC_A

Cuts at:      0    1930    2247 
   Size:       1930     317
 
BssHII G'CGCG_C

Cuts at:      0    1686    2247 
   Size:       1686     561
 
BstBI TT'CG_AA

Cuts at:      0     562    2247 
   Size:        562    1685
 
BstEII G'GTnAC_C

Cuts at:      0    1980    2247 
   Size:       1980     267
 
BstYI r'GATC_y

Cuts at:      0     525    1149    2101    2247 
   Size:        525     624     952     146

  Fragments arranged by size: 

                 952     624     525     146
 
Bsu36I CC'TnA_GG

Cuts at:      0     836    2204    2247 
   Size:        836    1368      43

  Fragments arranged by size: 

                1368     836      43
 
BtgI C'CryG_G

Cuts at:      0     498     658    1283    1355    2247 
   Size:        498     160     625      72     892

  Fragments arranged by size: 

                 892     625     498     160      72
 
BtsI GCAGTG_nn'

Cuts at:      0      56    2247 
   Size:         56    2191
 
EaeI y'GGCC_r

Cuts at:      0      27     710    2247 
   Size:         27     683    1537

  Fragments arranged by size: 

                1537     683      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     269     305     316     606     849     916    1010    1232 
   Size:        269      36      11     290     243      67      94     222

Cuts at:   1232    1450    1508    1556    1769    2247 
   Size:        218      58      48     213     478

  Fragments arranged by size: 

                 478     290     269     243     222     218     213      94
                  67      58      48      36      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     161    1096    1663    2247 
   Size:         19     142     935     567     584

  Fragments arranged by size: 

                 935     584     567     142      19
 
EcoICRI GAG'CTC

Cuts at:      0     637    1894    2247 
   Size:        637    1257     353

  Fragments arranged by size: 

                1257     637     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     611     761    1262    1280    1298    1316    1580 
   Size:         90     521     150     501      18      18      18     264

Cuts at:   1580    1810    1922    2247 
   Size:        230     112     325

  Fragments arranged by size: 

                 521     501     325     264     230     150     112      90
                  18      18      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2203    2247 
   Size:       2203      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2195    2247 
   Size:       2195      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2206    2247 
   Size:       2206      41
 
HaeII r_GCGC'y

Cuts at:      0    1663    1788    2247 
   Size:       1663     125     459

  Fragments arranged by size: 

                1663     459     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     523     555     803     835    1573    1605    2108    2140 
   Size:        523      32     248      32     738      32     503      32

Cuts at:   2140    2247 
   Size:        107

  Fragments arranged by size: 

                 738     523     503     248     107      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     678    2247 
   Size:        678    1569
 
HpaI GTT'AAC

Cuts at:      0     678    2247 
   Size:        678    1569
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     308    2247 
   Size:        308    1939
 
MscI TGG'CCA

Cuts at:      0     712    2247 
   Size:        712    1535
 
MslI CAynn'nnrTG

Cuts at:      0     769    1926    2247 
   Size:        769    1157     321

  Fragments arranged by size: 

                1157     769     321
 
MspA1I CmG'CkG

Cuts at:      0     201     730    1635    1641    2247 
   Size:        201     529     905       6     606

  Fragments arranged by size: 

                 905     606     529     201       6
 
NcoI C'CATG_G

Cuts at:      0     498     658    1283    1355    2247 
   Size:        498     160     625      72     892

  Fragments arranged by size: 

                 892     625     498     160      72
 
NheI G'CTAG_C

Cuts at:      0     240    2247 
   Size:        240    2007
 
NspI r_CATG'y

Cuts at:      0    1978    2247 
   Size:       1978     269
 
PciI A'CATG_T

Cuts at:      0    1974    2247 
   Size:       1974     273
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1264    2247 
   Size:       1264     983
 
PfoI T'CCnGG_A

Cuts at:      0    1944    2247 
   Size:       1944     303
 
PsiI TTA'TAA

Cuts at:      0    2086    2247 
   Size:       2086     161
 
PspOMI G'GGCC_C

Cuts at:      0    2194    2247 
   Size:       2194      53
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1827    1859    2201    2233    2247 
   Size:       1827      32     342      32      14

  Fragments arranged by size: 

                1827     342      32      32      14
 
PstI C_TGCA'G

Cuts at:      0     650    1413    1640    2238    2247 
   Size:        650     763     227     598       9

  Fragments arranged by size: 

                 763     650     598     227       9
 
PvuII CAG'CTG

Cuts at:      0     730    1635    2247 
   Size:        730     905     612

  Fragments arranged by size: 

                 905     730     612
 
SacI G_AGCT'C

Cuts at:      0     639    1896    2247 
   Size:        639    1257     351

  Fragments arranged by size: 

                1257     639     351
 
SapI GCTCTTCn'nnn_

Cuts at:      0     269     305     849    1232    1508    1769    2247 
   Size:        269      36     544     383     276     261     478

  Fragments arranged by size: 

                 544     478     383     276     269     261      36
 
SfcI C'TryA_G

Cuts at:      0     646    1409    1636    1844    2234    2247 
   Size:        646     763     227     208     390      13

  Fragments arranged by size: 

                 763     646     390     227     208      13
 
SmaI CCC'GGG

Cuts at:      0     211    1618    2247 
   Size:        211    1407     629

  Fragments arranged by size: 

                1407     629     211
 
SmlI C'TyrA_G

Cuts at:      0     394    1807    2247 
   Size:        394    1413     440

  Fragments arranged by size: 

                1413     440     394
 
StyI C'CwwG_G

Cuts at:      0     498     658    1283    1355    1886    2247 
   Size:        498     160     625      72     531     361

  Fragments arranged by size: 

                 625     531     498     361     160      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1041    1047    2247 
   Size:       1041       6    1200

  Fragments arranged by size: 

                1200    1041       6
 
TatI w'GTAC_w

Cuts at:      0     906    1930    2247 
   Size:        906    1024     317

  Fragments arranged by size: 

                1024     906     317
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     689     721    1898    1930    2247 
   Size:        689      32    1177      32     317

  Fragments arranged by size: 

                1177     689     317      32      32
 
XbaI T'CTAG_A

Cuts at:      0     793    2247 
   Size:        793    1454
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1353    2247 
   Size:       1353     894
 
XmaI C'CCGG_G

Cuts at:      0     209    1616    2247 
   Size:        209    1407     631

  Fragments arranged by size: 

                1407     631     209
 
XmnI GAAnn'nnTTC

Cuts at:      0    1762    2247 
   Size:       1762     485
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BclI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII     HpaI     MmeI     MscI
     MslI   MspA1I     NcoI     NheI     NspI     PciI    PflMI     PfoI
     PsiI   PspOMI     PsrI     PstI    PvuII     SacI     SapI     SfcI
     SmaI     SmlI     StyI    TaqII     TatI     TstI     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BmrI
    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
    BssSI   BstAPI    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XhoI     ZraI
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