Restriction map for pF1KE3123
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# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT14I     EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AfaI          868  GTAC                 GT^AC
AflIII        858  ACATGT               A^C[GA][CT]GT
AseI          573  ATTAAT               AT^TAAT
AsnI          573  ATTAAT               AT^TAAT
AvaII         834  GGACC                G^G[AT]CC
BamHI         435  GGATCC               G^GATCC
BbvI          901  GCAGC                GCAGC(8/12)
BciVI         912  GGATAC               GTATCC(6/5)
BclI          485  TGATCA               T^GATCA
BpmI          140  CTGGAG               CTGGAG(16/14)
BseRI          23  GAGGAG               GAGGAG(10/8)
BspLU11I      858  ACATGT               A^CATGT
BstBI         534  TTCGAA               TT^CGAA
BstYI         435  GGATCC               [GA]^GATC[CT]
DraI          925  TTTAAA               TTT^AAA
DraII         833  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          132  TGGCCG               [CT]^GGCC[GA]
EcoO109I      833  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         376  GATATC               GAT^ATC
FbaI          485  TGATCA               T^GATCA
Fnu4HI        901  GCAGC                GC^[ACGT]GC
HapII         313  CCGG                 C^CGG
HpaII         313  CCGG                 C^CGG
HphI          342  TCACC                GGTGA(8/7)
ItaI          901  GCAGC                GC^[ACGT]GC
MaeII         843  ACGT                 A^CGT
MflI          435  GGATCC               [GA]^GATC[CT]
MspA1I        805  CAGCTG               C[AC]G^C[GT]G
MspI          313  CCGG                 C^CGG
MwoI          651  GCTAAATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          858  ACATGT               [GA]CATG^[CT]
NspV          534  TTCGAA               TT^CGAA
PpuMI         833  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         573  ATTAAT               AT^TAAT
PstI           68  CTGCAG               CTGCA^G
PvuII         805  CAGCTG               CAG^CTG
RsaI          868  GTAC                 GT^AC
SfuI          534  TTCGAA               TT^CGAA
SspI          883  AATATT               AAT^ATT
TaqI          535  TCGA                 T^CGA
TseI          901  GCAGC                G^C[AT]GC
TthHB8I       535  TCGA                 T^CGA
XcmI          839  CCAAACGTCATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         435  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          178  GAATTT               [GA]^AATT[CT]
              654  AAATTT              
ApoI          178  GAATTT               [GA]^AATT[CT]
              654  AAATTT              
BstF5I        674  GGATG                GGATG(2/0)
              864  GGATG               
Cac8I         807  GCTGGC               GC[ACGT]^[ACGT]GC
              827  GCAAGC              
EarI           99  CTCTTC               CTCTTC(1/4)
              666  GAAGAG              
FokI          674  GGATG                GGATG(9/13)
              864  GGATG               
HinfI         537  GAATC                G^A[ACGT]TC
              724  GAATC               
Ksp632I        99  CTCTTC               CTCTTC(1/4)
              666  GAAGAG              
MaeIII        341  GTCAC                ^GT[ACGT]AC
              545  GTCAC               
MslI          130  CATGGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              859  CATGTGGATG          
TfiI          537  GAATC                G^A[AT]TC
              724  GAATC               
Tsp45I        341  GTCAC                ^GT[GC]AC
              545  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        AlwI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstNI       Cfr13I      DdeI        
DpnII       EcoRII      HaeIII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        Tru9I       Tsp509I     TspRI       
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