Restriction map for pF1KB7754
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AfaI        
AflII       AgeI        Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoNI       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1243  GACGTC               GACGT^C
AccIII       1387  TCCGGA               T^CCGGA
AcsI          990  GAATTC               [GA]^AATT[CT]
AhdI          631  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1172  AGCGCT               AGC^GCT
ApoI          990  GAATTC               [GA]^AATT[CT]
AspEI         631  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          972  CCCGGG               C^[CT]CG[GA]G
BalI         1038  TGGCCA               TGG^CCA
BbsI          368  GAAGAC               GAAGAC(2/6)
BglII         741  AGATCT               A^GATCT
BpuAI         368  GAAGAC               GAAGAC(2/6)
BsaI          645  GAGACC               GGTCTC(1/5)
BsaWI        1387  TCCGGA               [AT]^CCGG[AT]
BseAI        1387  TCCGGA               T^CCGGA
BsmBI         866  GAGACG               CGTCTC(1/5)
BsoBI         972  CCCGGG               C^[CT]CG[GA]G
BspEI        1387  TCCGGA               T^CCGGA
BspMI         350  ACCTGC               ACCTGC(4/8)
BstAPI        549  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1318  TTCGAA               TT^CGAA
DraIII        106  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1038  TGGCCA               [CT]^GGCC[GA]
Eam1105I      631  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1172  AGCGCT               AGC^GCT
EcoRI         990  GAATTC               G^AATTC
EcoRV         430  GATATC               GAT^ATC
FauI          854  GCGGG                CCCGC(4/6)
HaeII        1172  AGCGCT               [GA]GCGC^[CT]
HindIII       839  AAGCTT               A^AGCTT
MluNI        1038  TGGCCA               TGG^CCA
MroI         1387  TCCGGA               T^CCGGA
MscI         1038  TGGCCA               TGG^CCA
MseI         1447  TTAA                 T^TAA
NspV         1318  TTCGAA               TT^CGAA
PflMI         374  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          547  CTGCAG               CTGCA^G
PvuII         150  CAGCTG               CAG^CTG
SacI          295  GAGCTC               GAGCT^C
SapI          928  GAAGAGC              GCTCTTC(1/4)
SfuI         1318  TTCGAA               TT^CGAA
SmaI          972  CCCGGG               CCC^GGG
SmlI          935  CTCAAG               C^T[CT][GA]AG
Tru9I        1447  TTAA                 T^TAA
Van91I        374  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          650  CCAAGCAGACCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         972  CCCGGG               C^CCGGG
XmaI          972  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI         1060  CCGC                 CCGC(-3/-1)
              854  GCGG                
AcyI         1243  GACGTC               G[GA]^CG[CT]C
             1451  GACGCC              
AspHI         295  GAGCTC               G[AT]GC[AT]^C
             1369  GAGCAC              
BanII         295  GAGCTC               G[GA]GC[CT]^C
             1017  GAGCCC              
BbrPI         130  CACGTG               CAC^GTG
             1236  CACGTG              
BbvCI         718  CCTCAGC              CCTCAGC(-5/-2)
              216  GCTGAGG             
BfaI           31  CTAG                 C^TAG
             1111  CTAG                
BsaHI        1243  GACGTC               G[GA]^CG[CT]C
             1451  GACGCC              
BsgI          325  GTGCAG               GTGCAG(16/14)
              418  GTGCAG              
BsiHKAI       295  GAGCTC               G[AT]GC[AT]^C
             1369  GAGCAC              
BspHI         275  TCATGA               T^CATGA
              311  TCATGA              
BstEII        390  GGTCACC              G^GT[ACGT]ACC
              628  GGTGACC             
BstPI         390  GGTCACC              G^GT[ACGT]ACC
              628  GGTGACC             
BstXI         737  CCACAGATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              947  CCACACCTGTGG        
Cac8I         820  GCCTGC               GC[ACGT]^[ACGT]GC
             1015  GCGAGC              
CfoI         1173  GCGC                 GCG^C
             1377  GCGC                
DsaI           67  CCACGG               C^C[GA][CT]GG
              399  CCACGG              
EcoO65I       390  GGTCACC              G^GT[ACGT]ACC
              628  GGTGACC             
EcoT14I       659  CCTTGG               C^C[AT][AT]GG
             1049  CCAAGG              
HhaI         1173  GCGC                 GCG^C
             1377  GCGC                
Hin1I        1243  GACGTC               G[GA]^CG[CT]C
             1451  GACGCC              
HinP1I       1173  GCGC                 G^CGC
             1377  GCGC                
MaeI           31  CTAG                 C^TAG
             1111  CTAG                
MspA1I        150  CAGCTG               C[AC]G^C[GT]G
              852  CAGCGG              
NspI          254  ACATGC               [GA]CATG^[CT]
             1069  GCATGT              
PmaCI         130  CACGTG               CAC^GTG
             1236  CACGTG              
PmlI          130  CACGTG               CAC^GTG
             1236  CACGTG              
RcaI          275  TCATGA               T^CATGA
              311  TCATGA              
SfcI          547  CTGCAG               C^T[GA][CT]AG
             1350  CTACAG              
StyI          659  CCTTGG               C^C[AT][AT]GG
             1049  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AflIII      AluI        AlwI        AlwNI       Asp700I     
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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