Restriction map for pF1KB7808
 (Linear) (Six-base) MAPSORT of: KIBB7808.seq  Check: 4089  from: 1  to: 1785



KIBB7808.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AccI GT'mk_AC

Cuts at:      0     816     900    1785 
   Size:        816      84     885

  Fragments arranged by size: 

                 885     816      84
 
AclI AA'CG_TT

Cuts at:      0    1470    1785 
   Size:       1470     315
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     323     441    1197    1281    1469    1553    1633 
   Size:        171     152     118     756      84     188      84      80

Cuts at:   1633    1637    1785 
   Size:          4     148

  Fragments arranged by size: 

                 756     188     171     152     148     118      84      84
                  80       4
 
AflIII A'CryG_T

Cuts at:      0     880     987    1785 
   Size:        880     107     798

  Fragments arranged by size: 

                 880     798     107
 
ApoI r'AATT_y

Cuts at:      0     659    1056    1079    1759    1785 
   Size:        659     397      23     680      26

  Fragments arranged by size: 

                 680     659     397      26      23
 
AvrII C'CTAG_G

Cuts at:      0     345    1785 
   Size:        345    1440
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     570    1785 
   Size:        570    1215
 
BfrBI ATG'CAT

Cuts at:      0    1595    1713    1785 
   Size:       1595     118      72

  Fragments arranged by size: 

                1595     118      72
 
BglII A'GATC_T

Cuts at:      0    1216    1656    1785 
   Size:       1216     440     129

  Fragments arranged by size: 

                1216     440     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       1     250    1785 
   Size:          1     249    1535

  Fragments arranged by size: 

                1535     249       1
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1468    1785 
   Size:       1468     317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     689     773    1025    1193    1277    1379    1463 
   Size:        521     168      84     252     168      84     102      84

Cuts at:   1463    1549    1785 
   Size:         86     236

  Fragments arranged by size: 

                 521     252     236     168     168     102      86      84
                  84      84
 
BsaAI yAC'GTr

Cuts at:      0     881     990    1680    1785 
   Size:        881     109     690     105

  Fragments arranged by size: 

                 881     690     109     105
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484    1785 
   Size:        454      30    1301

  Fragments arranged by size: 

                1301     454      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61    1785 
   Size:         61    1724
 
BsiHKAI G_wGCw'C

Cuts at:      0     167    1785 
   Size:        167    1618
 
BsmI GAATG_Cn'

Cuts at:      0     874    1465    1597    1785 
   Size:        874     591     132     188

  Fragments arranged by size: 

                 874     591     188     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     167    1785 
   Size:        167    1618
 
BspHI T'CATG_A

Cuts at:      0    1155    1323    1511    1763    1785 
   Size:       1155     168     188     252      22

  Fragments arranged by size: 

                1155     252     188     168      22
 
BsrDI GCAATG_nn'

Cuts at:      0    1204    1560    1644    1785 
   Size:       1204     356      84     141

  Fragments arranged by size: 

                1204     356     141      84
 
BstBI TT'CG_AA

Cuts at:      0     561    1757    1785 
   Size:        561    1196      28

  Fragments arranged by size: 

                1196     561      28
 
BstYI r'GATC_y

Cuts at:      0     805    1216    1656    1740    1785 
   Size:        805     411     440      84      45

  Fragments arranged by size: 

                 805     440     411      84      45
 
BstZ17I GTA'TAC

Cuts at:      0     901    1785 
   Size:        901     884
 
BtsI GCAGTG_nn'

Cuts at:      0     952    1120    1785 
   Size:        952     168     665

  Fragments arranged by size: 

                 952     665     168
 
EarI CTCTTCn'nnn_

Cuts at:      0    1609    1785 
   Size:       1609     176
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     323     441     521     689     773    1025    1193 
   Size:        171     152     118      80     168      84     252     168

Cuts at:   1193    1197    1277    1281    1379    1463    1469    1549    1553 
   Size:          4      80       4      98      84       6      80       4

Cuts at:   1553    1633    1637    1785 
   Size:         80       4     148

  Fragments arranged by size: 

                 252     171     168     168     152     148     118      98
                  84      84      80      80      80      80       6       4
                   4       4       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     911    1785 
   Size:        911     874
 
EcoRI G'AATT_C

Cuts at:      0     659    1056    1759    1785 
   Size:        659     397     703      26

  Fragments arranged by size: 

                 703     659     397      26
 
EcoRV GAT'ATC

Cuts at:      0    1153    1509    1785 
   Size:       1153     356     276

  Fragments arranged by size: 

                1153     356     276
 
HincII GTy'rAC

Cuts at:      0     439    1785 
   Size:        439    1346
 
HindIII A'AGCT_T

Cuts at:      0     293     321    1785 
   Size:        293      28    1464

  Fragments arranged by size: 

                1464     293      28
 
MslI CAynn'nnrTG

Cuts at:      0     432     554     574     684     879    1785 
   Size:        432     122      20     110     195     906

  Fragments arranged by size: 

                 906     432     195     122     110      20
 
NdeI CA'TA_TG

Cuts at:      0     399    1785 
   Size:        399    1386
 
NsiI A_TGCA'T

Cuts at:      0    1597    1715    1785 
   Size:       1597     118      70

  Fragments arranged by size: 

                1597     118      70
 
NspI r_CATG'y

Cuts at:      0    1717    1785 
   Size:       1717      68
 
PfoI T'CCnGG_A

Cuts at:      0     281    1785 
   Size:        281    1504
 
PmlI CAC'GTG

Cuts at:      0     881    1785 
   Size:        881     904
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138    1785 
   Size:        106      32    1647

  Fragments arranged by size: 

                1647     106      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     911    1785 
   Size:        911     874
 
PsiI TTA'TAA

Cuts at:      0     599    1373    1785 
   Size:        599     774     412

  Fragments arranged by size: 

                 774     599     412
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1481    1513    1785 
   Size:       1481      32     272

  Fragments arranged by size: 

                1481     272      32
 
SfcI C'TryA_G

Cuts at:      0      84     125    1785 
   Size:         84      41    1660

  Fragments arranged by size: 

                1660      84      41
 
StyI C'CwwG_G

Cuts at:      0     345    1785 
   Size:        345    1440
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55    1785 
   Size:         55    1730
 
XmnI GAAnn'nnTTC

Cuts at:      0     622     659     827    1079    1210    1294    1566    1650 
   Size:        622      37     168     252     131      84     272      84

Cuts at:   1650    1734    1785 
   Size:         84      51

  Fragments arranged by size: 

                 622     272     252     168     131      84      84      84
                  51      37
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     ApoI    AvrII     BbsI    BfrBI
    BglII     BmrI     BplI     BpmI    BsaAI    BsaXI    BseRI  BsiHKAI
     BsmI Bsp1286I    BspHI    BsrDI    BstBI    BstYI  BstZ17I     BtsI
     EarI  Eco57MI EcoO109I    EcoRI    EcoRV   HincII  HindIII     MslI
     NdeI     NsiI     NspI     PfoI     PmlI     PpiI    PpuMI     PsiI
     PsrI     SfcI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI     FalI     FseI     FspI    FspAI    HaeII    Hin4I     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI    PshAI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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