Restriction map for pF1KB8418
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspMI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MroI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Alw44I        376  GTGCAC               G^TGCAC
ApaLI         376  GTGCAC               G^TGCAC
AseI          667  ATTAAT               AT^TAAT
AsnI          667  ATTAAT               AT^TAAT
AspHI         376  GTGCAC               G[AT]GC[AT]^C
BbsI          121  GTCTTC               GAAGAC(2/6)
BbvI          267  GCAGC                GCAGC(8/12)
BclI          155  TGATCA               T^GATCA
BmyI          376  GTGCAC               G[AGT]GC[ACT]^C
BpuAI         121  GTCTTC               GAAGAC(2/6)
BsiHKAI       376  GTGCAC               G[AT]GC[AT]^C
BsmFI           3  GGGAC                GGGAC(10/14)
Bsp1286I      376  GTGCAC               G[AGT]GC[ACT]^C
BsrDI          54  GCAATG               GCAATG(2/0)
BstYI         279  AGATCC               [GA]^GATC[CT]
EcoT14I       184  CCTTGG               C^C[AT][AT]GG
FbaI          155  TGATCA               T^GATCA
Fnu4HI        267  GCAGC                GC^[ACGT]GC
HincII       1251  GTTAAC               GT[CT]^[GA]AC
HindII       1251  GTTAAC               GT[CT]^[GA]AC
HpaI         1251  GTTAAC               GTT^AAC
ItaI          267  GCAGC                GC^[ACGT]GC
MaeIII       2383  GTGAC                ^GT[ACGT]AC
MflI          279  AGATCC               [GA]^GATC[CT]
PshBI         667  ATTAAT               AT^TAAT
SexAI         116  ACCTGGT              A^CC[AT]GGT
SphI          551  GCATGC               GCATG^C
StyI          184  CCTTGG               C^C[AT][AT]GG
TseI          267  GCAGC                G^C[AT]GC
Tsp45I       2383  GTGAC                ^GT[GC]AC
XhoII         279  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        691  ACATGT               A^C[GA][CT]GT
             1027  ACATGT              
AlwI          280  GATCC                GGATC(4/5)
             2150  GATCC               
BalI           26  TGGCCA               TGG^CCA
              259  TGGCCA              
BfaI          565  CTAG                 C^TAG
              758  CTAG                
BsaJI         126  CCTGGG               C^C[ACGT][ACGT]GG
              184  CCTTGG              
BsmAI         201  GAGAC                GTCTC(1/5)
              139  GTCTC               
Bsp1407I      333  TGTACA               T^GTACA
             1114  TGTACA              
BspLU11I      691  ACATGT               A^CATGT
             1027  ACATGT              
BsrFI        1139  GCCGGT               [GA]^CCGG[CT]
             1475  GCCGGT              
BsrGI         333  TGTACA               T^GTACA
             1114  TGTACA              
Cac8I         414  GCCAGC               GC[ACGT]^[ACGT]GC
              551  GCATGC              
Cfr10I       1139  GCCGGT               [GA]^CCGG[CT]
             1475  GCCGGT              
DraI          339  TTTAAA               TTT^AAA
             2059  TTTAAA              
EaeI           26  TGGCCA               [CT]^GGCC[GA]
              259  TGGCCA              
HapII        1140  CCGG                 C^CGG
             1476  CCGG                
HinfI        1085  GAATC                G^A[ACGT]TC
             1674  GATTC               
HpaII        1140  CCGG                 C^CGG
             1476  CCGG                
HphI         1126  GGTGA                GGTGA(8/7)
              158  TCACC               
MaeI          565  CTAG                 C^TAG
              758  CTAG                
MluNI          26  TGGCCA               TGG^CCA
              259  TGGCCA              
MscI           26  TGGCCA               TGG^CCA
              259  TGGCCA              
MslI          325  CATAAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              352  CATAAAAGTG          
MspI         1140  CCGG                 C^CGG
             1476  CCGG                
MwoI          183  GCCTTGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              261  GCCAGAGCAGC         
NlaIV           3  GGGACC               GG[ACGT]^[ACGT]CC
              189  GGAGCC              
PstI         1094  CTGCAG               CTGCA^G
             1430  CTGCAG              
SspBI         333  TGTACA               T^GTACA
             1114  TGTACA              
SspI          432  AATATT               AAT^ATT
             2318  AATATT              
TfiI         1085  GAATC                G^A[AT]TC
             1674  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AvaII       
BpmI        BseRI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       EcoRI       EcoRII      FokI        HaeIII      
HindIII     MboI        MboII       MnlI        MseI        
MvaI        NdeII       NlaIII      NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        Tru9I       
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com