Restriction map for pF1KA1789
 (Linear) (Six-base) MAPSORT of: KIAA1789.seq  Check: 1052  from: 1  to: 1401



KIAA1789.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1052    1401 
   Size:       1037      15     349

  Fragments arranged by size: 

                1037     349      15
 
AflII C'TTAA_G

Cuts at:      0    1264    1401 
   Size:       1264     137
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     284     316    1401 
   Size:        284      32    1085

  Fragments arranged by size: 

                1085     284      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     956    1401 
   Size:        956     445
 
ApoI r'AATT_y

Cuts at:      0     120    1401 
   Size:        120    1281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     136     388    1401 
   Size:        136     252    1013

  Fragments arranged by size: 

                1013     252     136
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     483    1401 
   Size:        483     918
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1002    1401 
   Size:       1002     399
 
BsaWI w'CCGG_w

Cuts at:      0      56     317    1401 
   Size:         56     261    1084

  Fragments arranged by size: 

                1084     261      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     284     314    1401 
   Size:        284      30    1087

  Fragments arranged by size: 

                1087     284      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1246    1401 
   Size:       1246     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     196     712    1401 
   Size:        179      17     516     689

  Fragments arranged by size: 

                 689     516     179      17
 
BspEI T'CCGG_A

Cuts at:      0     317    1401 
   Size:        317    1084
 
BspHI T'CATG_A

Cuts at:      0     903    1401 
   Size:        903     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1072    1401 
   Size:       1072     329
 
BsrDI GCAATG_nn'

Cuts at:      0     359     888    1401 
   Size:        359     529     513

  Fragments arranged by size: 

                 529     513     359
 
BstYI r'GATC_y

Cuts at:      0     447    1401 
   Size:        447     954
 
EarI CTCTTCn'nnn_

Cuts at:      0     218    1056    1099    1358    1401 
   Size:        218     838      43     259      43

  Fragments arranged by size: 

                 838     259     218      43      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1052    1401 
   Size:       1037      15     349

  Fragments arranged by size: 

                1037     349      15
 
EcoRI G'AATT_C

Cuts at:      0     120    1401 
   Size:        120    1281
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     424     688    1401 
   Size:        424     264     713

  Fragments arranged by size: 

                 713     424     264
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     245     277     975    1007    1232    1264    1285    1317 
   Size:        245      32     698      32     225      32      21      32

Cuts at:   1317    1401 
   Size:         84

  Fragments arranged by size: 

                 698     245     225      84      32      32      32      32
                  21
 
HincII GTy'rAC

Cuts at:      0     943    1401 
   Size:        943     458
 
HindIII A'AGCT_T

Cuts at:      0    1261    1401 
   Size:       1261     140
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     785    1401 
   Size:        785     616
 
MslI CAynn'nnrTG

Cuts at:      0     172     688    1401 
   Size:        172     516     713

  Fragments arranged by size: 

                 713     516     172
 
NdeI CA'TA_TG

Cuts at:      0     387     651    1401 
   Size:        387     264     750

  Fragments arranged by size: 

                 750     387     264
 
NspI r_CATG'y

Cuts at:      0      69    1401 
   Size:         69    1332
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     284     316    1401 
   Size:        284      32    1085

  Fragments arranged by size: 

                1085     284      32
 
SfcI C'TryA_G

Cuts at:      0     749    1401 
   Size:        749     652
 
SmlI C'TyrA_G

Cuts at:      0     981    1264    1401 
   Size:        981     283     137

  Fragments arranged by size: 

                 981     283     137
 
SspI AAT'ATT

Cuts at:      0     342    1401 
   Size:        342    1059
 
TatI w'GTAC_w

Cuts at:      0     426    1401 
   Size:        426     975
 

 Enzymes that do cut:

     AcuI    AflII     AloI    AlwNI     ApoI     BbsI    BciVI    BpuEI
    BsaWI    BsaXI    BseRI     BsgI    BspEI    BspHI    BspMI    BsrDI
    BstYI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII  HindIII
     MmeI     MslI     NdeI     NspI     PpiI     SfcI     SmlI     SspI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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