Restriction map for pF1KE0340
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# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        Ksp632I     
MluI        MroI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          535  GTAGAC               GT^[AC][GT]AC
AflIII       1030  ACACGT               A^C[GA][CT]GT
AocI         1066  CCTCAGG              CC^T[ACGT]AGG
AspHI         295  GTGCTC               G[AT]GC[AT]^C
AvaI         1276  CTCGGG               C^[CT]CG[GA]G
AvaII         493  GGTCC                G^G[AT]CC
BbsI         1005  GTCTTC               GAAGAC(2/6)
BclI          734  TGATCA               T^GATCA
BpuAI        1005  GTCTTC               GAAGAC(2/6)
BsaBI        1335  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         169  CTCCTC               GAGGAG(10/8)
BsiHKAI       295  GTGCTC               G[AT]GC[AT]^C
BsiWI        1033  CGTACG               C^GTACG
BsmBI         592  GAGACG               CGTCTC(1/5)
BsoBI        1276  CTCGGG               C^[CT]CG[GA]G
BspHI        1202  TCATGA               T^CATGA
BspMI        1052  GCAGGT               ACCTGC(4/8)
BssSI         875  CTCGTG               CACGAG(-5/-1)
Bsu36I       1066  CCTCAGG              CC^T[ACGT]AGG
Cac8I         140  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         622  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        940  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           51  CCGCGG               C^C[GA][CT]GG
Eco81I       1066  CCTCAGG              CC^T[ACGT]AGG
EcoNI         663  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      622  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I         4  CCTTGG               C^C[AT][AT]GG
FauI           34  CCCGC                CCCGC(4/6)
FbaI          734  TGATCA               T^GATCA
HaeII         722  AGCGCC               [GA]GCGC^[CT]
KspI           51  CCGCGG               CCGC^GG
MamI         1335  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1346  CAATTG               C^AATTG
MslI         1031  CACGTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1346  CAATTG               C^AATTG
NlaIV          30  GGTTCC               GG[ACGT]^[ACGT]CC
PvuII         712  CAGCTG               CAG^CTG
RcaI         1202  TCATGA               T^CATGA
SacII          51  CCGCGG               CCGC^GG
SmlI          260  CTCAAG               C^T[CT][GA]AG
StyI            4  CCTTGG               C^C[AT][AT]GG
XbaI         1092  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
              497  CGCG                
AcsI          559  GAATTT               [GA]^AATT[CT]
              859  GAATTT              
AlwNI         658  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              709  CAGCAGCTG           
ApoI          559  GAATTT               [GA]^AATT[CT]
              859  GAATTT              
BalI         1039  TGGCCA               TGG^CCA
             1266  TGGCCA              
BanII         872  GGGCTC               G[GA]GC[CT]^C
             1351  GAGCCC              
BcnI          870  CCGGG                CC^[GC]GG
             1354  CCCGG               
BfaI          553  CTAG                 C^TAG
             1093  CTAG                
BglI          413  GCCTCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1228  GCCTTCAAGGC         
BsaAI        1031  CACGTA               [CT]AC^GT[GA]
             1035  TACGTG              
BstUI          52  CGCG                 CG^CG
              497  CGCG                
BstYI         468  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              
CfoI          723  GCGC                 GCG^C
              881  GCGC                
EaeI         1039  TGGCCA               [CT]^GGCC[GA]
             1266  TGGCCA              
HhaI          723  GCGC                 GCG^C
              881  GCGC                
HinP1I        723  GCGC                 G^CGC
              881  GCGC                
HindIII      1137  AAGCTT               A^AGCTT
             1183  AAGCTT              
MaeI          553  CTAG                 C^TAG
             1093  CTAG                
MflI          468  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              
MluNI        1039  TGGCCA               TGG^CCA
             1266  TGGCCA              
MscI         1039  TGGCCA               TGG^CCA
             1266  TGGCCA              
MseI         1187  TTAA                 T^TAA
             1214  TTAA                
MvnI           52  CGCG                 CG^CG
              497  CGCG                
NciI          870  CCGGG                CC^[GC]GG
             1354  CCCGG               
NspI          630  ACATGC               [GA]CATG^[CT]
             1304  ACATGC              
PflMI         282  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1042  CCAGTCAATGG         
SfaNI        1161  GATGC                GCATC(5/9)
             1384  GATGC               
TaqI          477  TCGA                 T^CGA
             1242  TCGA                
TfiI          120  GATTC                G^A[AT]TC
              377  GATTC               
Tru9I        1187  TTAA                 T^TAA
             1214  TTAA                
Tsp45I       1022  GTGAC                ^GT[GC]AC
             1438  GTGAC               
TspRI         287  CAGTG                CA[GC]TG[ACGT][ACGT]^
              507  CACTG               
TthHB8I       477  TCGA                 T^CGA
             1242  TCGA                
Van91I        282  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1042  CCAGTCAATGG         
XhoII         468  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Asp700I     
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TseI        
Tsp509I     XmnI        
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