Restriction map for pF1KB5459
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvrII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MfeI        MluI        MroI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          361  GTAGAC               GT^[AC][GT]AC
AflIII        610  ACATGT               A^C[GA][CT]GT
AocI          316  CCTCAGG              CC^T[ACGT]AGG
AvaI          393  CCCGAG               C^[CT]CG[GA]G
AvaII         627  GGTCC                G^G[AT]CC
AviII         739  TGCGCA               TGC^GCA
BanI          471  GGCACC               G^G[CT][GA]CC
BanII        1035  GAGCCC               G[GA]GC[CT]^C
BclI          266  TGATCA               T^GATCA
BcnI           87  CCGGG                CC^[GC]GG
BfaI         1054  CTAG                 C^TAG
BglI          640  GCCATTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         960  AGATCT               A^GATCT
BmyI         1035  GAGCCC               G[AGT]GC[ACT]^C
BsaI          273  GAGACC               GGTCTC(1/5)
BsgI         1168  GTGCAG               GTGCAG(16/14)
BsoBI         393  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1035  GAGCCC               G[AGT]GC[ACT]^C
BspHI         269  TCATGA               T^CATGA
BspLU11I      610  ACATGT               A^CATGT
BsrI          368  ACTGG                ACTGG(1/-1)
BssSI         116  CTCGTG               CACGAG(-5/-1)
Bsu36I        316  CCTCAGG              CC^T[ACGT]AGG
DraII         321  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        316  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      321  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       680  CCAAGG               C^C[AT][AT]GG
FbaI          266  TGATCA               T^GATCA
FspI          739  TGCGCA               TGC^GCA
HapII          87  CCGG                 C^CGG
HpaII          87  CCGG                 C^CGG
MaeI         1054  CTAG                 C^TAG
MaeIII        112  GTTAC                ^GT[ACGT]AC
MspI           87  CCGG                 C^CGG
NciI           87  CCGGG                CC^[GC]GG
PleI          690  GACTC                GAGTC(4/5)
RcaI          269  TCATGA               T^CATGA
SspI          818  AATATT               AAT^ATT
StuI          781  AGGCCT               AGG^CCT
StyI          680  CCAAGG               C^C[AT][AT]GG
TfiI          563  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BalI          672  TGGCCA               TGG^CCA
              926  TGGCCA              
BbsI          217  GAAGAC               GAAGAC(2/6)
              235  GAAGAC              
BpuAI         217  GAAGAC               GAAGAC(2/6)
              235  GAAGAC              
BsaBI         261  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              582  GATTTCCATC          
BseRI         989  CTCCTC               GAGGAG(10/8)
             1046  CTCCTC              
BsmFI         733  GGGAC                GGGAC(10/14)
              628  GTCCC               
BspMI         206  ACCTGC               ACCTGC(4/8)
              872  ACCTGC              
CfoI          210  GCGC                 GCG^C
              740  GCGC                
FauI          650  CCCGC                CCCGC(4/6)
              528  GCGGG               
HhaI          210  GCGC                 GCG^C
              740  GCGC                
HinP1I        210  GCGC                 G^CGC
              740  GCGC                
HinfI         563  GATTC                G^A[ACGT]TC
              690  GACTC               
MamI          261  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              582  GATTTCCATC          
MluNI         672  TGGCCA               TGG^CCA
              926  TGGCCA              
MscI          672  TGGCCA               TGG^CCA
              926  TGGCCA              
MseI           41  TTAA                 T^TAA
              466  TTAA                
SmlI          407  CTCAAG               C^T[CT][GA]AG
             1073  CTCAAG              
TaqI          546  TCGA                 T^CGA
              810  TCGA                
Tru9I          41  TTAA                 T^TAA
              466  TTAA                
TspRI          66  CAGTG                CA[GC]TG[ACGT][ACGT]^
              184  CAGTG               
TthHB8I       546  TCGA                 T^CGA
              810  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BamHI       
BbvI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MboI        MboII       MflI        MnlI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        Tsp509I     XhoII       
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