Restriction map for
pF1KE0307
(Linear) (Six-base) MAPSORT of: KIEE0307.seq Check: 4787 from: 1 to: 2052
KIEE0307.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 2048 2052
Size: 2048 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 338 682 703 1015 1066 1709 2052
Size: 188 150 344 21 312 51 643 343
Fragments arranged by size:
643 344 343 312 188 150 51 21
AlwNI CAG_nnn'CTG
Cuts at: 0 924 2052
Size: 924 1128
ApaI G_GGCC'C
Cuts at: 0 2009 2052
Size: 2009 43
ApaLI G'TGCA_C
Cuts at: 0 151 2052
Size: 151 1901
ApoI r'AATT_y
Cuts at: 0 575 1105 2052
Size: 575 530 947
Fragments arranged by size:
947 575 530
BanI G'GyrC_C
Cuts at: 0 1932 2052
Size: 1932 120
BanII G_rGCy'C
Cuts at: 0 244 374 872 1949 2009 2052
Size: 244 130 498 1077 60 43
Fragments arranged by size:
1077 498 244 130 60 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 2052
Size: 197 1855
BbvCI CC'TCA_GC
Cuts at: 0 1998 2052
Size: 1998 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1123 1157 2052
Size: 1123 34 895
Fragments arranged by size:
1123 895 34
BclI T'GATC_A
Cuts at: 0 1797 2052
Size: 1797 255
BglI GCCn_nnn'nGGC
Cuts at: 0 1846 1953 2052
Size: 1846 107 99
Fragments arranged by size:
1846 107 99
BlpI GC'TnA_GC
Cuts at: 0 1073 2052
Size: 1073 979
Bme1580I G_kGCm'C
Cuts at: 0 155 708 2009 2052
Size: 155 553 1301 43
Fragments arranged by size:
1301 553 155 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 68 115 1943 2052
Size: 68 47 1828 109
Fragments arranged by size:
1828 109 68 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 2052
Size: 640 1412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 109 368 1880 2052
Size: 35 74 259 1512 172
Fragments arranged by size:
1512 259 172 74 35
Bpu10I CC'TnA_GC
Cuts at: 0 1998 2052
Size: 1998 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1597 2052
Size: 961 636 455
Fragments arranged by size:
961 636 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 1780 2052
Size: 1780 272
BsaAI yAC'GTr
Cuts at: 0 709 2052
Size: 709 1343
BsaBI GATnn'nnATC
Cuts at: 0 524 1306 2052
Size: 524 782 746
Fragments arranged by size:
782 746 524
BsaHI Gr'CG_yC
Cuts at: 0 1604 1804 2045 2052
Size: 1604 200 241 7
Fragments arranged by size:
1604 241 200 7
BsaWI w'CCGG_w
Cuts at: 0 471 1831 2052
Size: 471 1360 221
Fragments arranged by size:
1360 471 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 2052
Size: 634 30 1388
Fragments arranged by size:
1388 634 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 892 988 1486 1670 1993 2052
Size: 544 348 96 498 184 323 59
Fragments arranged by size:
544 498 348 323 184 96 59
BseYI C'CCAG_C
Cuts at: 0 2002 2009 2052
Size: 2002 7 43
Fragments arranged by size:
2002 43 7
BsiHKAI G_wGCw'C
Cuts at: 0 155 872 1117 1791 1919 2052
Size: 155 717 245 674 128 133
Fragments arranged by size:
717 674 245 155 133 128
BsmI GAATG_Cn'
Cuts at: 0 276 2052
Size: 276 1776
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1370 1449 2052
Size: 1370 79 603
Fragments arranged by size:
1370 603 79
Bsp1286I G_dGCh'C
Cuts at: 0 155 244 374 708 872 1117 1791 1919
Size: 155 89 130 334 164 245 674 128
Cuts at: 1919 1949 2009 2052
Size: 30 60 43
Fragments arranged by size:
674 334 245 164 155 130 128 89
60 43 30
BspHI T'CATG_A
Cuts at: 0 1520 2052
Size: 1520 532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 446 2052
Size: 446 1606
BsrBI CCG'CTC
Cuts at: 0 1609 2052
Size: 1609 443
BssSI C'ACGA_G
Cuts at: 0 1964 2052
Size: 1964 88
Bsu36I CC'TnA_GG
Cuts at: 0 1682 2052
Size: 1682 370
BtgI C'CryG_G
Cuts at: 0 1928 2052
Size: 1928 124
BtsI GCAGTG_nn'
Cuts at: 0 1710 2052
Size: 1710 342
ClaI AT'CG_AT
Cuts at: 0 857 2052
Size: 857 1195
DraI TTT'AAA
Cuts at: 0 900 2052
Size: 900 1152
EaeI y'GGCC_r
Cuts at: 0 102 1508 2052
Size: 102 1406 544
Fragments arranged by size:
1406 544 102
EarI CTCTTCn'nnn_
Cuts at: 0 164 314 324 683 713 1256 1340 1421
Size: 164 150 10 359 30 543 84 81
Cuts at: 1421 1907 2052
Size: 486 145
Fragments arranged by size:
543 486 359 164 150 145 84 81
30 10
EcoICRI GAG'CTC
Cuts at: 0 870 2052
Size: 870 1182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 109 188 338 368 682 703 1015
Size: 35 74 79 150 30 314 21 312
Cuts at: 1015 1066 1709 1880 2052
Size: 51 643 171 172
Fragments arranged by size:
643 314 312 172 171 150 79 74
51 35 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 2052
Size: 91 1961
EcoO109I rG'GnC_Cy
Cuts at: 0 1839 1994 2006 2052
Size: 1839 155 12 46
Fragments arranged by size:
1839 155 46 12
EcoRI G'AATT_C
Cuts at: 0 1105 2052
Size: 1105 947
EcoRV GAT'ATC
Cuts at: 0 162 2052
Size: 162 1890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 712 2052
Size: 712 1340
FspI TGC'GCA
Cuts at: 0 1905 2052
Size: 1905 147
HaeII r_GCGC'y
Cuts at: 0 697 2052
Size: 697 1355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 1420 1452 1515 1547 1789 1821
Size: 49 32 1339 32 63 32 242 32
Cuts at: 1821 2052
Size: 231
Fragments arranged by size:
1339 242 231 63 49 32 32 32
32
HincII GTy'rAC
Cuts at: 0 44 1544 2049 2052
Size: 44 1500 505 3
Fragments arranged by size:
1500 505 44 3
HindIII A'AGCT_T
Cuts at: 0 937 1826 2052
Size: 937 889 226
Fragments arranged by size:
937 889 226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 150 1229 1764 2052
Size: 139 11 1079 535 288
Fragments arranged by size:
1079 535 288 139 11
MscI TGG'CCA
Cuts at: 0 104 1510 2052
Size: 104 1406 542
Fragments arranged by size:
1406 542 104
MspA1I CmG'CkG
Cuts at: 0 31 186 702 2002 2052
Size: 31 155 516 1300 50
Fragments arranged by size:
1300 516 155 50 31
NcoI C'CATG_G
Cuts at: 0 1928 2052
Size: 1928 124
NspI r_CATG'y
Cuts at: 0 1904 1943 2052
Size: 1904 39 109
Fragments arranged by size:
1904 109 39
PfoI T'CCnGG_A
Cuts at: 0 1807 2052
Size: 1807 245
PmlI CAC'GTG
Cuts at: 0 709 2052
Size: 709 1343
PshAI GACnn'nnGTC
Cuts at: 0 1406 2052
Size: 1406 646
PspOMI G'GGCC_C
Cuts at: 0 2005 2052
Size: 2005 47
PstI C_TGCA'G
Cuts at: 0 665 917 1070 2052
Size: 665 252 153 982
Fragments arranged by size:
982 665 252 153
PvuII CAG'CTG
Cuts at: 0 186 2002 2052
Size: 186 1816 50
Fragments arranged by size:
1816 186 50
SacI G_AGCT'C
Cuts at: 0 872 2052
Size: 872 1180
SalI G'TCGA_C
Cuts at: 0 2047 2052
Size: 2047 5
SapI GCTCTTCn'nnn_
Cuts at: 0 164 1421 1907 2052
Size: 164 1257 486 145
Fragments arranged by size:
1257 486 164 145
SbfI CC_TGCA'GG
Cuts at: 0 1070 2052
Size: 1070 982
SfcI C'TryA_G
Cuts at: 0 661 913 1066 1867 2052
Size: 661 252 153 801 185
Fragments arranged by size:
801 661 252 185 153
SmlI C'TyrA_G
Cuts at: 0 940 1612 2052
Size: 940 672 440
Fragments arranged by size:
940 672 440
SphI G_CATG'C
Cuts at: 0 1904 1943 2052
Size: 1904 39 109
Fragments arranged by size:
1904 109 39
SspI AAT'ATT
Cuts at: 0 896 1000 2052
Size: 896 104 1052
Fragments arranged by size:
1052 896 104
StuI AGG'CCT
Cuts at: 0 1861 2052
Size: 1861 191
StyI C'CwwG_G
Cuts at: 0 1724 1928 2052
Size: 1724 204 124
Fragments arranged by size:
1724 204 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 372 2052
Size: 372 1680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 634 666 2052
Size: 634 32 1386
Fragments arranged by size:
1386 634 32
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApaLI ApoI BanI BanII
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BssSI Bsu36I BtgI BtsI ClaI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MmeI
MscI MspA1I NcoI NspI PfoI PmlI PshAI PspOMI
PstI PvuII SacI SalI SapI SbfI SfcI SmlI
SphI SspI StuI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BciVI BfrBI BglII BmgBI
BmtI BsgI BsiEI BsiWI BspEI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
CspCI DraIII DrdI EagI EciI FseI FspAI HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacII SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI