Restriction map for pF1KE0175
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BcgI        BclI        
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfcI        SfoI        SfuI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          475  AAATTC               [GA]^AATT[CT]
AfaI          798  GTAC                 GT^AC
AhdI          734  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          475  AAATTC               [GA]^AATT[CT]
AspEI         734  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI           4  GAGCTC               G[AT]GC[AT]^C
AvaI          220  CCCGAG               C^[CT]CG[GA]G
BbsI          865  GTCTTC               GAAGAC(2/6)
BbvCI         695  CCTCAGC              CCTCAGC(-5/-2)
BciVI         519  GGATAC               GTATCC(6/5)
BglII         418  AGATCT               A^GATCT
BpmI          311  CTGGAG               CTGGAG(16/14)
BpuAI         865  GTCTTC               GAAGAC(2/6)
BsaAI         109  TACGTA               [CT]AC^GT[GA]
BseRI         493  GAGGAG               GAGGAG(10/8)
BsgI          386  GTGCAG               GTGCAG(16/14)
BsiHKAI         4  GAGCTC               G[AT]GC[AT]^C
BsoBI         220  CCCGAG               C^[CT]CG[GA]G
BsrBI         663  CCGCTC               CCGCTC(-3/-3)
BsrI          301  ACTGG                ACTGG(1/-1)
BstYI         418  AGATCT               [GA]^GATC[CT]
CfoI          143  GCGC                 GCG^C
DraIII        196  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          163  GACACATCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          524  CCGTGG               C^C[GA][CT]GG
EaeI          716  CGGCCA               [CT]^GGCC[GA]
Eam1105I      734  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          21  CCTCATTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         142  GGCGCT               [GA]GCGC^[CT]
HhaI          143  GCGC                 GCG^C
HinP1I        143  GCGC                 G^CGC
MaeII         110  ACGT                 A^CGT
MflI          418  AGATCT               [GA]^GATC[CT]
MslI          459  CATCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          186  GCATGT               [GA]CATG^[CT]
PvuII         680  CAGCTG               CAG^CTG
RsaI          798  GTAC                 GT^AC
SacI            4  GAGCTC               GAGCT^C
SapI          537  GAAGAGC              GCTCTTC(1/4)
SexAI         197  ACCTGGT              A^CC[AT]GGT
SfaNI         345  GCATC                GCATC(5/9)
SfiI          708  GGCCCCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          340  CTTGAG               C^T[CT][GA]AG
SnaBI         109  TACGTA               TAC^GTA
StuI          363  AGGCCT               AGG^CCT
XcmI          839  CCACTGTCTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         418  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         133  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              830  CAGCACCTG           
AvaII          87  GGTCC                G^G[AT]CC
              192  GGACC               
BanI          529  GGTGCC               G^G[CT][GA]CC
              762  GGTGCC              
BanII           4  GAGCTC               G[GA]GC[CT]^C
              331  GAGCCC              
BglI          700  GCCTCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              709  GCCCCCTCGGC         
BsmAI         293  GAGAC                GTCTC(1/5)
              844  GTCTC               
DpnII         419  GATC                 ^GATC
              579  GATC                
DraII         407  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              690  AGGCCCC             
EarI          271  GAAGAG               CTCTTC(1/4)
              537  GAAGAG              
EcoO109I      407  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              690  AGGCCCC             
FauI          611  CCCGC                CCCGC(4/6)
              277  GCGGG               
Ksp632I       271  GAAGAG               CTCTTC(1/4)
              537  GAAGAG              
MaeIII         16  GTCAC                ^GT[ACGT]AC
              859  GTCAC               
MboI          419  GATC                 ^GATC
              579  GATC                
MspA1I        120  CAGCGG               C[AC]G^C[GT]G
              680  CAGCTG              
NdeII         419  GATC                 ^GATC
              579  GATC                
NlaIII        187  CATG                 CATG^
              759  CATG                
PflMI         132  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              195  CCACCTGGTGG         
PleI          295  GACTC                GAGTC(4/5)
              857  GAGTC               
Sau3AI        419  GATC                 ^GATC
              579  GATC                
Tsp45I         16  GTCAC                ^GT[GC]AC
              859  GTCAC               
Van91I        132  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              195  CCACCTGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BspMI       
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MboII       MnlI        MvaI        MwoI        NlaIV       
Sau96I      ScrFI       StyI        TfiI        TseI        
Tsp509I     TspRI       
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