Restriction map for
pF1KE0175
(Circular) (Six-base) MAPSORT of: pF1KE0175.seq Check: 7412 from: 1 to: 4016
pF1KE0175 4016 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 157 481 550 951 157
Size: 324 69 401 3222
Fragments arranged by size:
3222 401 324 69
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 664 963 1841 2273 3020 3288 398
Size: 266 299 878 432 747 268 1126
Fragments arranged by size:
1126 878 747 432 299 268 266
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AhdI GACnn_n'nnGTC
Cuts at: 847 847
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 246 943 1321 2888 246
Size: 697 378 1567 1374
Fragments arranged by size:
1567 1374 697 378
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 583 1020 3369 3625 583
Size: 437 2349 256 974
Fragments arranged by size:
2349 974 437 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 328 1036 328
Size: 708 3308
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 637 870 1032 1446 1694 1729 637
Size: 233 162 414 248 35 2924
Fragments arranged by size:
2924 414 248 233 162 35
BanII G_rGCy'C
Cuts at: 116 443 1030 2060 116
Size: 327 587 1030 2072
Fragments arranged by size:
2072 1030 587 327
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 966 966
Size: 4016
BbvCI CC'TCA_GC
Cuts at: 804 804
Size: 4016
BciVI GTATCCnnnnn_n'
Cuts at: 621 1914 2675 4016 621
Size: 1293 761 1341 621
Fragments arranged by size:
1341 1293 761 621
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglI GCCn_nnn'nGGC
Cuts at: 814 823 814
Size: 9 4007
BglII A'GATC_T
Cuts at: 526 1531 3568 526
Size: 1005 2037 974
Fragments arranged by size:
2037 1005 974
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 642 1641 1734 2790 642
Size: 999 93 1056 1868
Fragments arranged by size:
1868 1056 999 93
BmrI ACTGGGnnnn_n'
Cuts at: 419 1228 1309 1644 3788 419
Size: 809 81 335 2144 647
Fragments arranged by size:
2144 809 647 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 440
Size: 4016
Bpu10I CC'TnA_GC
Cuts at: 804 804
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1192 2071 2563 2861 469
Size: 723 879 492 298 1624
Fragments arranged by size:
1624 879 723 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 219 1369 219
Size: 1150 2866
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 956 986 956
Size: 30 3986
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 616
Size: 4016
BseYI C'CCAG_C
Cuts at: 424 442 553 714 1441 2199 2776 3272 424
Size: 18 111 161 727 758 577 496 1168
Fragments arranged by size:
1168 758 727 577 496 161 111 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 515
Size: 4016
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 116 1030 1808 1998 2790 116
Size: 914 778 190 792 1342
Fragments arranged by size:
1342 914 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 116 443 642 1030 1641 1734 1808 1998 2060
Size: 327 199 388 611 93 74 190 62
Cuts at: 2060 2790 116
Size: 730 1342
Fragments arranged by size:
1342 730 611 388 327 199 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 481 550 951 1066 1280 1582 1963 157
Size: 324 69 401 115 214 302 381 2210
Fragments arranged by size:
2210 401 381 324 302 214 115 69
BsrBI CCG'CTC
Cuts at: 50 773 2308 2362 3846 4011 50
Size: 723 1535 54 1484 165 55
Fragments arranged by size:
1535 1484 723 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 526 1041 1508 1531 1866 2112 3124 3210
Size: 525 515 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 525 515 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 632 107
Size: 525 3491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 724 762 1259 724
Size: 38 497 3481
Fragments arranged by size:
3481 497 38
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DraIII CAC_nnn'GTG
Cuts at: 309 309
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 277 1722 2580 277
Size: 1445 858 1713
Fragments arranged by size:
1713 1445 858
EaeI y'GGCC_r
Cuts at: 824 1601 1775 2166 2193 3538 824
Size: 777 174 391 27 1345 1302
Fragments arranged by size:
1345 1302 777 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 374 640 2039 2249 374
Size: 266 1399 210 2141
Fragments arranged by size:
2141 1399 266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 114 1028 114
Size: 914 3102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 440 664 963 1841 2273 3020 3288 398
Size: 42 224 299 878 432 747 268 1126
Fragments arranged by size:
1126 878 747 432 299 268 224 42
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 4016
EcoO109I rG'GnC_Cy
Cuts at: 516 799 1155 516
Size: 283 356 3377
Fragments arranged by size:
3377 356 283
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 254 1450 1698 2720 3699 254
Size: 1196 248 1022 979 571
Fragments arranged by size:
1196 1022 979 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 833 865 956 988 1858 1888 1890 1920 833
Size: 32 91 32 870 30 2 30 2929
Fragments arranged by size:
2929 870 91 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2871 2687
Size: 184 3832
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 571 2005 2132 571
Size: 1434 127 2455
Fragments arranged by size:
2455 1434 127
MspA1I CmG'CkG
Cuts at: 230 790 1127 1441 1801 2814 3059 230
Size: 560 337 314 360 1013 245 1187
Fragments arranged by size:
1187 1013 560 360 337 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 107
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 298 1069 1569 2476 298
Size: 771 500 907 1838
Fragments arranged by size:
1838 907 771 500
PasI CC'CwG_GG
Cuts at: 781 781
Size: 4016
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PflMI CCAn_nnn'nTGG
Cuts at: 246 309 246
Size: 63 3953
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PstI C_TGCA'G
Cuts at: 1063 1748 1063
Size: 685 3331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 790 1441 1801 790
Size: 651 360 3005
Fragments arranged by size:
3005 651 360
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 116 1030 116
Size: 914 3102
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 640 2039 2249 640
Size: 1399 210 2407
Fragments arranged by size:
2407 1399 210
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SexAI A'CCwGG_T
Cuts at: 305 305
Size: 4016
SfcI C'TryA_G
Cuts at: 33 1059 1744 2737 2928 33
Size: 1026 685 993 191 1121
Fragments arranged by size:
1121 1026 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 823 823
Size: 4016
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 448 1171 2086 2578 2840 448
Size: 723 915 492 262 1624
Fragments arranged by size:
1624 915 723 492 262
SnaBI TAC'GTA
Cuts at: 219 219
Size: 4016
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StuI AGG'CCT
Cuts at: 473 473
Size: 4016
StyI C'CwwG_G
Cuts at: 107 191 318 468 812 1150 1504 107
Size: 84 127 150 344 338 354 2619
Fragments arranged by size:
2619 354 344 338 150 127 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 625 852 883 1599 1732 2191 2400 625
Size: 227 31 716 133 459 209 2241
Fragments arranged by size:
2241 716 459 227 209 133 31
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 1511 954
Size: 557 3459
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SgrAI
SrfI SspI SwaI XhoI XmnI ZraI