Restriction map for pF1KE0175
 (Linear) (Six-base) MAPSORT of: KIEE0175.seq  Check: 4567  from: 1  to: 903



KIEE0175.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      49     373     442     843     903 
   Size:         49     324      69     401      60

  Fragments arranged by size: 

                 401     324      69      60      49
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     556     855     903 
   Size:        290     266     299      48

  Fragments arranged by size: 

                 299     290     266      48
 
AhdI GACnn_n'nnGTC

Cuts at:      0     739     903 
   Size:        739     164
 
AlwNI CAG_nnn'CTG

Cuts at:      0     138     835     903 
   Size:        138     697      68

  Fragments arranged by size: 

                 697     138      68
 
ApoI r'AATT_y

Cuts at:      0     475     903 
   Size:        475     428
 
AvaI C'yCGr_G

Cuts at:      0     220     903 
   Size:        220     683
 
BanI G'GyrC_C

Cuts at:      0     529     762     903 
   Size:        529     233     141

  Fragments arranged by size: 

                 529     233     141
 
BanII G_rGCy'C

Cuts at:      0       8     335     903 
   Size:          8     327     568

  Fragments arranged by size: 

                 568     327       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     858     903 
   Size:        858      45
 
BbvCI CC'TCA_GC

Cuts at:      0     696     903 
   Size:        696     207
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     513     903 
   Size:        513     390
 
BglI GCCn_nnn'nGGC

Cuts at:      0     706     715     903 
   Size:        706       9     188

  Fragments arranged by size: 

                 706     188       9
 
BglII A'GATC_T

Cuts at:      0     418     903 
   Size:        418     485
 
Bme1580I G_kGCm'C

Cuts at:      0     534     903 
   Size:        534     369
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     311     903 
   Size:        311     592
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     903 
   Size:        332     571
 
Bpu10I CC'TnA_GC

Cuts at:      0     696     903 
   Size:        696     207
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     903 
   Size:        361     542
 
BsaAI yAC'GTr

Cuts at:      0     111     903 
   Size:        111     792
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     848     878     903 
   Size:        848      30      25

  Fragments arranged by size: 

                 848      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     508     903 
   Size:        508     395
 
BseYI C'CCAG_C

Cuts at:      0     316     334     445     606     903 
   Size:        316      18     111     161     297

  Fragments arranged by size: 

                 316     297     161     111      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     903 
   Size:        407     496
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     903 
   Size:          8     895
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     335     534     903 
   Size:          8     327     199     369

  Fragments arranged by size: 

                 369     327     199       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      49     373     442     843     903 
   Size:         49     324      69     401      60

  Fragments arranged by size: 

                 401     324      69      60      49
 
BsrBI CCG'CTC

Cuts at:      0     665     903 
   Size:        665     238
 
BstYI r'GATC_y

Cuts at:      0     418     903 
   Size:        418     485
 
BtgI C'CryG_G

Cuts at:      0     524     903 
   Size:        524     379
 
BtsI GCAGTG_nn'

Cuts at:      0     616     654     903 
   Size:        616      38     249

  Fragments arranged by size: 

                 616     249      38
 
DraIII CAC_nnn'GTG

Cuts at:      0     201     903 
   Size:        201     702
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     169     903 
   Size:        169     734
 
EaeI y'GGCC_r

Cuts at:      0     716     903 
   Size:        716     187
 
EarI CTCTTCn'nnn_

Cuts at:      0     266     532     903 
   Size:        266     266     371

  Fragments arranged by size: 

                 371     266     266
 
EcoICRI GAG'CTC

Cuts at:      0       6     903 
   Size:          6     897
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     332     556     855     903 
   Size:        290      42     224     299      48

  Fragments arranged by size: 

                 299     290     224      48      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     903 
   Size:         25     878
 
EcoO109I rG'GnC_Cy

Cuts at:      0     408     691     903 
   Size:        408     283     212

  Fragments arranged by size: 

                 408     283     212
 
HaeII r_GCGC'y

Cuts at:      0     146     903 
   Size:        146     757
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     725     757     848     880     903 
   Size:        725      32      91      32      23

  Fragments arranged by size: 

                 725      91      32      32      23
 
MslI CAynn'nnrTG

Cuts at:      0     463     903 
   Size:        463     440
 
MspA1I CmG'CkG

Cuts at:      0     122     682     903 
   Size:        122     560     221

  Fragments arranged by size: 

                 560     221     122
 
NspI r_CATG'y

Cuts at:      0     190     903 
   Size:        190     713
 
PasI CC'CwG_GG

Cuts at:      0     673     903 
   Size:        673     230
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138     201     903 
   Size:        138      63     702

  Fragments arranged by size: 

                 702     138      63
 
PvuII CAG'CTG

Cuts at:      0     682     903 
   Size:        682     221
 
SacI G_AGCT'C

Cuts at:      0       8     903 
   Size:          8     895
 
SapI GCTCTTCn'nnn_

Cuts at:      0     532     903 
   Size:        532     371
 
SexAI A'CCwGG_T

Cuts at:      0     197     903 
   Size:        197     706
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     715     903 
   Size:        715     188
 
SmlI C'TyrA_G

Cuts at:      0     340     903 
   Size:        340     563
 
SnaBI TAC'GTA

Cuts at:      0     111     903 
   Size:        111     792
 
StuI AGG'CCT

Cuts at:      0     365     903 
   Size:        365     538
 
StyI C'CwwG_G

Cuts at:      0      83     210     360     704     903 
   Size:         83     127     150     344     199

  Fragments arranged by size: 

                 344     199     150     127      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     517     744     775     903 
   Size:        517     227      31     128

  Fragments arranged by size: 

                 517     227     128      31
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     846     903 
   Size:        846      57
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApoI     AvaI     BanI    BanII
     BbsI    BbvCI    BciVI     BglI    BglII Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BstYI     BtgI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     MslI   MspA1I     NspI     PasI    PflMI    PvuII     SacI     SapI
    SexAI     SfiI     SmlI    SnaBI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI     BlpI
    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     EagI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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