Restriction map for pF1KE0207
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# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AluI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
AfaI          132  GTAC                 GT^AC
AflIII        622  ACACGT               A^C[GA][CT]GT
AlwNI         668  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
BclI          242  TGATCA               T^GATCA
BfaI          215  CTAG                 C^TAG
BsaAI         623  CACGTA               [CT]AC^GT[GA]
BsiWI         131  CGTACG               C^GTACG
BsmAI         386  GTCTC                GTCTC(1/5)
BsmI          342  GAATGC               GAATGC(1/-1)
BspMI         247  ACCTGC               ACCTGC(4/8)
BsrI          679  ACTGG                ACTGG(1/-1)
BstAPI        667  GCAGTCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          54  CGCG                 CG^CG
DpnII         243  GATC                 ^GATC
DrdI          476  GACCTCTGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          500  CCATGG               C^C[GA][CT]GG
EcoT14I       500  CCATGG               C^C[AT][AT]GG
EcoT22I       289  ATGCAT               ATGCA^T
FbaI          242  TGATCA               T^GATCA
MaeI          215  CTAG                 C^TAG
MboI          243  GATC                 ^GATC
MseI          211  TTAA                 T^TAA
MspA1I         25  CCGCTG               C[AC]G^C[GT]G
MvnI           54  CGCG                 CG^CG
NcoI          500  CCATGG               C^CATGG
NdeII         243  GATC                 ^GATC
NsiI          289  ATGCAT               ATGCA^T
PflMI         495  CCAACCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         188  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          588  CTGCAG               CTGCA^G
RsaI          132  GTAC                 GT^AC
Sau3AI        243  GATC                 ^GATC
SfcI          588  CTGCAG               C^T[GA][CT]AG
SmaI          100  CCCGGG               CCC^GGG
StuI          108  AGGCCT               AGG^CCT
StyI          500  CCATGG               C^C[AT][AT]GG
TfiI          446  GAATC                G^A[AT]TC
Tru9I         211  TTAA                 T^TAA
Van91I        495  CCAACCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         100  CCCGGG               C^CCGGG
XmaI          100  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

ApaI           76  GGGCCC               GGGCC^C
               97  GGGCCC              
AvaI          100  CCCGGG               C^[CT]CG[GA]G
              157  CCCGAG              
AvaII         189  GGACC                G^G[AT]CC
              475  GGACC               
BsoBI         100  CCCGGG               C^[CT]CG[GA]G
              157  CCCGAG              
BsrDI         526  CATTGC               GCAATG(2/0)
              320  GCAATG              
CfoI           53  GCGC                 GCG^C
              612  GCGC                
DdeI           35  CTGAG                C^T[ACGT]AG
              481  CTGAG               
HhaI           53  GCGC                 GCG^C
              612  GCGC                
HinP1I         53  GCGC                 G^CGC
              612  GCGC                
HphI          205  TCACC                GGTGA(8/7)
              245  TCACC               
MslI          270  CATCAACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              292  CATCAGCATG          
PleI          254  GAGTC                GAGTC(4/5)
              483  GAGTC               
SphI          421  GCATGC               GCATG^C
              705  GCATGC              
TaqI           44  TCGA                 T^CGA
              369  TCGA                
Tsp45I        403  GTGAC                ^GT[GC]AC
              670  GTCAC               
TthHB8I        44  TCGA                 T^CGA
              369  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeII       MaeIII      MnlI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       NspI        
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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