Restriction map for pF1KE0207
 (Linear) (Six-base) MAPSORT of: KIEE0207.seq  Check: 4706  from: 1  to: 831



KIEE0207.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     256     831 
   Size:        256     575
 
AflIII A'CryG_T

Cuts at:      0     622     831 
   Size:        622     209
 
AlwNI CAG_nnn'CTG

Cuts at:      0     673     831 
   Size:        673     158
 
ApaI G_GGCC'C

Cuts at:      0      80     101     831 
   Size:         80      21     730

  Fragments arranged by size: 

                 730      80      21
 
AvaI C'yCGr_G

Cuts at:      0     100     157     831 
   Size:        100      57     674

  Fragments arranged by size: 

                 674     100      57
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     609     642     831 
   Size:        609      33     189

  Fragments arranged by size: 

                 609     189      33
 
BanII G_rGCy'C

Cuts at:      0      41      80     101     783     831 
   Size:         41      39      21     682      48

  Fragments arranged by size: 

                 682      48      41      39      21
 
BclI T'GATC_A

Cuts at:      0     242     831 
   Size:        242     589
 
BfrBI ATG'CAT

Cuts at:      0     291     831 
   Size:        291     540
 
Bme1580I G_kGCm'C

Cuts at:      0      80     101     237     831 
   Size:         80      21     136     594

  Fragments arranged by size: 

                 594     136      80      21
 
BsaAI yAC'GTr

Cuts at:      0     625     831 
   Size:        625     206
 
BseYI C'CCAG_C

Cuts at:      0      94     831 
   Size:         94     737
 
BsiWI C'GTAC_G

Cuts at:      0     131     831 
   Size:        131     700
 
BsmI GAATG_Cn'

Cuts at:      0     348     831 
   Size:        348     483
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      80     101     237     783     831 
   Size:         41      39      21     136     546      48

  Fragments arranged by size: 

                 546     136      48      41      39      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     256     831 
   Size:        256     575
 
BsrDI GCAATG_nn'

Cuts at:      0     327     525     831 
   Size:        327     198     306

  Fragments arranged by size: 

                 327     306     198
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     673     831 
   Size:        673     158
 
BtgI C'CryG_G

Cuts at:      0     500     831 
   Size:        500     331
 
BtsI GCAGTG_nn'

Cuts at:      0     585     671     831 
   Size:        585      86     160

  Fragments arranged by size: 

                 585     160      86
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     666     701     831 
   Size:        666      35     130

  Fragments arranged by size: 

                 666     130      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     482     831 
   Size:        482     349
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76     189     460     831 
   Size:         76     113     271     371

  Fragments arranged by size: 

                 371     271     113      76
 
MslI CAynn'nnrTG

Cuts at:      0     274     296     831 
   Size:        274      22     535

  Fragments arranged by size: 

                 535     274      22
 
MspA1I CmG'CkG

Cuts at:      0      27     831 
   Size:         27     804
 
NcoI C'CATG_G

Cuts at:      0     500     831 
   Size:        500     331
 
NsiI A_TGCA'T

Cuts at:      0     293     831 
   Size:        293     538
 
NspI r_CATG'y

Cuts at:      0     301     425     709     831 
   Size:        301     124     284     122

  Fragments arranged by size: 

                 301     284     124     122
 
PasI CC'CwG_GG

Cuts at:      0     352     831 
   Size:        352     479
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     501     831 
   Size:        501     330
 
PfoI T'CCnGG_A

Cuts at:      0     471     831 
   Size:        471     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     831 
   Size:        189     642
 
PspOMI G'GGCC_C

Cuts at:      0      76      97     831 
   Size:         76      21     734

  Fragments arranged by size: 

                 734      76      21
 
PstI C_TGCA'G

Cuts at:      0     592     831 
   Size:        592     239
 
SfcI C'TryA_G

Cuts at:      0     588     831 
   Size:        588     243
 
SmaI CCC'GGG

Cuts at:      0     102     831 
   Size:        102     729
 
SphI G_CATG'C

Cuts at:      0     425     709     831 
   Size:        425     284     122

  Fragments arranged by size: 

                 425     284     122
 
StuI AGG'CCT

Cuts at:      0     110     831 
   Size:        110     721
 
StyI C'CwwG_G

Cuts at:      0     500     831 
   Size:        500     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     493     831 
   Size:        493     338
 
XmaI C'CCGG_G

Cuts at:      0     100     831 
   Size:        100     731
 

 Enzymes that do cut:

     AarI   AflIII    AlwNI     ApaI     AvaI     BaeI    BanII     BclI
    BfrBI Bme1580I    BsaAI    BseYI    BsiWI     BsmI Bsp1286I    BspMI
    BsrDI   BstAPI     BtgI     BtsI    CspCI     DrdI EcoO109I     MslI
   MspA1I     NcoI     NsiI     NspI     PasI    PflMI     PfoI    PpuMI
   PspOMI     PstI     SfcI     SmaI     SphI     StuI     StyI    TaqII
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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