Restriction map for pF1KE0418
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BanI        BbeI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII          20  CGCG                 CG^CG
AcsI          304  GAATTC               [GA]^AATT[CT]
AlwNI        1143  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          304  GAATTC               [GA]^AATT[CT]
BamHI         221  GGATCC               G^GATCC
BbrPI         292  CACGTG               CAC^GTG
BglI          661  GCCACGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         292  CACGTG               [CT]AC^GT[GA]
BsiEI          27  CGGCCG               CG[GA][CT]^CG
BsmBI         826  CGTCTC               CGTCTC(1/5)
BsmI         1089  GAATGC               GAATGC(1/-1)
Bsp1407I     1073  TGTACA               T^GTACA
BsrBI         131  GAGCGG               CCGCTC(-3/-3)
BsrFI        1014  GCCGGC               [GA]^CCGG[CT]
BsrGI        1073  TGTACA               T^GTACA
BstEII        138  GGTGACC              G^GT[ACGT]ACC
BstPI         138  GGTGACC              G^GT[ACGT]ACC
BstUI          20  CGCG                 CG^CG
BstXI         152  CCAGCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           21  GCGC                 GCG^C
Cfr10I       1014  GCCGGC               [GA]^CCGG[CT]
DraIII        868  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           27  CGGCCG               C^GGCCG
EarI          643  GAAGAG               CTCTTC(1/4)
EclXI          27  CGGCCG               C^GGCCG
Eco52I         27  CGGCCG               C^GGCCG
EcoO65I       138  GGTGACC              G^GT[ACGT]ACC
EcoRI         304  GAATTC               G^AATTC
EcoT14I       164  CCAAGG               C^C[AT][AT]GG
HhaI           21  GCGC                 GCG^C
HinP1I         21  GCGC                 G^CGC
Ksp632I       643  GAAGAG               CTCTTC(1/4)
MaeII         293  ACGT                 A^CGT
MvnI           20  CGCG                 CG^CG
NaeI         1014  GCCGGC               GCC^GGC
NgoMIV       1014  GCCGGC               G^CCGGC
PmaCI         292  CACGTG               CAC^GTG
PmlI          292  CACGTG               CAC^GTG
PstI          712  CTGCAG               CTGCA^G
PvuII         531  CAGCTG               CAG^CTG
SmaI          298  CCCGGG               CCC^GGG
SspBI        1073  TGTACA               T^GTACA
StuI           84  AGGCCT               AGG^CCT
StyI          164  CCAAGG               C^C[AT][AT]GG
TaqI          726  TCGA                 T^CGA
TfiI         1007  GAATC                G^A[AT]TC
TthHB8I       726  TCGA                 T^CGA
XmaCI         298  CCCGGG               C^CCGGG
XmaI          298  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          196  GACGCC               G[GA]^CG[CT]C
              824  GGCGTC              
ApaI           62  GGGCCC               GGGCC^C
              745  GGGCCC              
Asp700I       718  GAAACCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1204  GAAATTGTTC          
AvaI          298  CCCGGG               C^[CT]CG[GA]G
              495  CCCGAG              
BbsI          568  GAAGAC               GAAGAC(2/6)
              681  GAAGAC              
BcgI          111  CGATAGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               10  GCAGTGTGGTCG        
BpuAI         568  GAAGAC               GAAGAC(2/6)
              681  GAAGAC              
BsaHI         196  GACGCC               G[GA]^CG[CT]C
              824  GGCGTC              
BsaI          262  GGTCTC               GGTCTC(1/5)
              312  GGTCTC              
BseRI         484  CTCCTC               GAGGAG(10/8)
              806  CTCCTC              
BsoBI         298  CCCGGG               C^[CT]CG[GA]G
              495  CCCGAG              
BsrI          218  ACTGG                ACTGG(1/-1)
              244  ACTGG               
BssSI         663  CACGAG               CACGAG(-5/-1)
              397  CTCGTG              
BstYI         221  GGATCC               [GA]^GATC[CT]
              986  AGATCC              
DpnII         222  GATC                 ^GATC
              987  GATC                
DrdI          406  GACCTTGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              571  GACTCCGTGGTC        
HgaI          196  GACGC                GACGC(5/10)
              825  GCGTC               
Hin1I         196  GACGCC               G[GA]^CG[CT]C
              824  GGCGTC              
MboI          222  GATC                 ^GATC
              987  GATC                
MflI          221  GGATCC               [GA]^GATC[CT]
              986  AGATCC              
NdeII         222  GATC                 ^GATC
              987  GATC                
PleI          571  GACTC                GAGTC(4/5)
             1185  GACTC               
Sau3AI        222  GATC                 ^GATC
              987  GATC                
SfcI          528  CTACAG               C^T[GA][CT]AG
              712  CTGCAG              
SmlI          508  CTCAAG               C^T[CT][GA]AG
              829  CTCAAG              
XhoII         221  GGATCC               [GA]^GATC[CT]
              986  AGATCC              
XmnI          718  GAAACCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1204  GAAATTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BalI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PflMI       PpuMI       RsaI        Sau96I      ScrFI       
SfaNI       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        
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