Restriction map for pF1KE0418
 (Linear) (Six-base) MAPSORT of: KIEE0418.seq  Check: 1256  from: 1  to: 1269



KIEE0418.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     727     897     921    1269 
   Size:        587     140     170      24     348

  Fragments arranged by size: 

                 587     348     170     140      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1148    1269 
   Size:       1148     121
 
ApaI G_GGCC'C

Cuts at:      0      66     749    1269 
   Size:         66     683     520

  Fragments arranged by size: 

                 683     520      66
 
ApoI r'AATT_y

Cuts at:      0     304    1269 
   Size:        304     965
 
AvaI C'yCGr_G

Cuts at:      0     298     495    1269 
   Size:        298     197     774

  Fragments arranged by size: 

                 774     298     197
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     961     994    1269 
   Size:        961      33     275

  Fragments arranged by size: 

                 961     275      33
 
BamHI G'GATC_C

Cuts at:      0     221    1269 
   Size:        221    1048
 
BanII G_rGCy'C

Cuts at:      0      66     749     805    1269 
   Size:         66     683      56     464

  Fragments arranged by size: 

                 683     464      66      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     575     688    1269 
   Size:        575     113     581

  Fragments arranged by size: 

                 581     575     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      33     100     134    1269 
   Size:         33      67      34    1135

  Fragments arranged by size: 

                1135      67      34      33
 
BglI GCCn_nnn'nGGC

Cuts at:      0     667    1269 
   Size:        667     602
 
Bme1580I G_kGCm'C

Cuts at:      0      66     299     749    1269 
   Size:         66     233     450     520

  Fragments arranged by size: 

                 520     450     233      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     824    1149    1269 
   Size:        824     325     120

  Fragments arranged by size: 

                 824     325     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     871     987    1201    1269 
   Size:        871     116     214      68

  Fragments arranged by size: 

                 871     214     116      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     814    1269 
   Size:        493     321     455

  Fragments arranged by size: 

                 493     455     321
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     268     318    1269 
   Size:        268      50     951

  Fragments arranged by size: 

                 951     268      50
 
BsaAI yAC'GTr

Cuts at:      0     294    1269 
   Size:        294     975
 
BsaHI Gr'CG_yC

Cuts at:      0     197     825    1269 
   Size:        197     628     444

  Fragments arranged by size: 

                 628     444     197
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     122     152    1269 
   Size:        122      30    1117

  Fragments arranged by size: 

                1117     122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475     797    1269 
   Size:        475     322     472

  Fragments arranged by size: 

                 475     472     322
 
BseYI C'CCAG_C

Cuts at:      0     442     533    1269 
   Size:        442      91     736

  Fragments arranged by size: 

                 736     442      91
 
BsiEI CG_ry'CG

Cuts at:      0      30    1269 
   Size:         30    1239
 
BsmI GAATG_Cn'

Cuts at:      0    1095    1269 
   Size:       1095     174
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     832    1269 
   Size:        832     437
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     299     749     805    1269 
   Size:         66     233     450      56     464

  Fragments arranged by size: 

                 464     450     233      66      56
 
BsrBI CCG'CTC

Cuts at:      0     133    1269 
   Size:        133    1136
 
BsrFI r'CCGG_y

Cuts at:      0    1014    1269 
   Size:       1014     255
 
BsrGI T'GTAC_A

Cuts at:      0    1073    1269 
   Size:       1073     196
 
BssSI C'ACGA_G

Cuts at:      0     397     663    1269 
   Size:        397     266     606

  Fragments arranged by size: 

                 606     397     266
 
BstEII G'GTnAC_C

Cuts at:      0     138    1269 
   Size:        138    1131
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     159    1269 
   Size:        159    1110
 
BstYI r'GATC_y

Cuts at:      0     221     986    1269 
   Size:        221     765     283

  Fragments arranged by size: 

                 765     283     221
 
BtgI C'CryG_G

Cuts at:      0     125     575     878    1221    1269 
   Size:        125     450     303     343      48

  Fragments arranged by size: 

                 450     343     303     125      48
 
BtsI GCAGTG_nn'

Cuts at:      0      17     326    1269 
   Size:         17     309     943

  Fragments arranged by size: 

                 943     309      17
 
DraIII CAC_nnn'GTG

Cuts at:      0     873    1269 
   Size:        873     396
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     412     577    1269 
   Size:        412     165     692

  Fragments arranged by size: 

                 692     412     165
 
EaeI y'GGCC_r

Cuts at:      0      27      77     149     161     288     359     881    1269 
   Size:         27      50      72      12     127      71     522     388

  Fragments arranged by size: 

                 522     388     127      72      71      50      27      12
            
 
EagI C'GGCC_G

Cuts at:      0      27    1269 
   Size:         27    1242
 
EarI CTCTTCn'nnn_

Cuts at:      0     638    1269 
   Size:        638     631
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     649     687    1269 
   Size:        649      38     582

  Fragments arranged by size: 

                 649     582      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     727     871     897     921     987    1201    1269 
   Size:        587     140     144      26      24      66     214      68

  Fragments arranged by size: 

                 587     214     144     140      68      66      26      24
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     405     551     605     745     918    1269 
   Size:         63     342     146      54     140     173     351

  Fragments arranged by size: 

                 351     342     173     146     140      63      54
 
EcoRI G'AATT_C

Cuts at:      0     304    1269 
   Size:        304     965
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     534    1269 
   Size:        534     735
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1174    1206    1269 
   Size:       1174      32      63

  Fragments arranged by size: 

                1174      63      32
 
MscI TGG'CCA

Cuts at:      0     163     361     883    1269 
   Size:        163     198     522     386

  Fragments arranged by size: 

                 522     386     198     163
 
MslI CAynn'nnrTG

Cuts at:      0     736    1054    1075    1269 
   Size:        736     318      21     194

  Fragments arranged by size: 

                 736     318     194      21
 
MspA1I CmG'CkG

Cuts at:      0     193     234     334     533     849    1269 
   Size:        193      41     100     199     316     420

  Fragments arranged by size: 

                 420     316     199     193     100      41
 
NaeI GCC'GGC

Cuts at:      0    1016    1269 
   Size:       1016     253
 
NgoMIV G'CCGG_C

Cuts at:      0    1014    1269 
   Size:       1014     255
 
PasI CC'CwG_GG

Cuts at:      0      59     922    1269 
   Size:         59     863     347

  Fragments arranged by size: 

                 863     347      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     357     741    1135    1269 
   Size:        357     384     394     134

  Fragments arranged by size: 

                 394     384     357     134
 
PmlI CAC'GTG

Cuts at:      0     294    1269 
   Size:        294     975
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      86     118    1269 
   Size:         86      32    1151

  Fragments arranged by size: 

                1151      86      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     405     551     605     918    1269 
   Size:        405     146      54     313     351

  Fragments arranged by size: 

                 405     351     313     146      54
 
PsiI TTA'TAA

Cuts at:      0     587    1269 
   Size:        587     682
 
PspOMI G'GGCC_C

Cuts at:      0      62     745    1269 
   Size:         62     683     524

  Fragments arranged by size: 

                 683     524      62
 
PstI C_TGCA'G

Cuts at:      0     716    1269 
   Size:        716     553
 
PvuII CAG'CTG

Cuts at:      0     533    1269 
   Size:        533     736
 
SanDI GG'GwC_CC

Cuts at:      0     551     918    1269 
   Size:        551     367     351

  Fragments arranged by size: 

                 551     367     351
 
SfcI C'TryA_G

Cuts at:      0     528     712    1269 
   Size:        528     184     557

  Fragments arranged by size: 

                 557     528     184
 
SmaI CCC'GGG

Cuts at:      0     300    1269 
   Size:        300     969
 
SmlI C'TyrA_G

Cuts at:      0     508     829    1269 
   Size:        508     321     440

  Fragments arranged by size: 

                 508     440     321
 
StuI AGG'CCT

Cuts at:      0      86    1269 
   Size:         86    1183
 
StyI C'CwwG_G

Cuts at:      0     164    1269 
   Size:        164    1105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     433    1269 
   Size:        433     836
 
TatI w'GTAC_w

Cuts at:      0    1073    1269 
   Size:       1073     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     122     154     847     859     879     891    1269 
   Size:        122      32     693      12      20      12     378

  Fragments arranged by size: 

                 693     378     122      32      20      12      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132     369    1162    1269 
   Size:        132     237     793     107

  Fragments arranged by size: 

                 793     237     132     107
 
XmaI C'CCGG_G

Cuts at:      0     298    1269 
   Size:        298     971
 
XmnI GAAnn'nnTTC

Cuts at:      0     722    1208    1269 
   Size:        722     486      61

  Fragments arranged by size: 

                 722     486      61
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI    BanII
     BbsI     BcgI     BglI Bme1580I     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI     BsmI    BsmBI
 Bsp1286I    BsrBI    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI
     BtgI     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI    Hin4I     MscI     MslI   MspA1I
     NaeI   NgoMIV     PasI    PflMI     PmlI     PpiI    PpuMI     PsiI
   PspOMI     PstI    PvuII    SanDI     SfcI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BsaBI    BsaWI
     BsgI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrDI   BssHII
   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI    PshAI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com