Restriction map for pF1KE0517
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NarI        NcoI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        Sau3AI      SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AfaI          177  GTAC                 GT^AC
Aor51HI       374  AGCGCT               AGC^GCT
AvaI           99  CCCGAG               C^[CT]CG[GA]G
BalI          119  TGGCCA               TGG^CCA
BanI          547  GGTGCC               G^G[CT][GA]CC
BcnI          165  CCCGG                CC^[GC]GG
BlpI          909  GCTGAGC              GC^T[ACGT]AGC
BpmI          499  CTGGAG               CTGGAG(16/14)
Bpu1102I      909  GCTGAGC              GC^T[ACGT]AGC
BseRI         205  GAGGAG               GAGGAG(10/8)
BsmBI          49  GAGACG               CGTCTC(1/5)
BsoBI          99  CCCGAG               C^[CT]CG[GA]G
BstAPI        795  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         909  GCTGAGC              GC^T[ACGT]AGC
EarI          451  GAAGAG               CTCTTC(1/4)
Eco47III      374  AGCGCT               AGC^GCT
EcoNI         750  CCTTGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         239  GATATC               GAT^ATC
EcoT14I       542  CCTTGG               C^C[AT][AT]GG
Ksp632I       451  GAAGAG               CTCTTC(1/4)
KspI           60  CCGCGG               CCGC^GG
MboII         451  GAAGA                GAAGA(8/7)
MluNI         119  TGGCCA               TGG^CCA
MscI          119  TGGCCA               TGG^CCA
NciI          165  CCCGG                CC^[GC]GG
NspI          885  GCATGT               [GA]CATG^[CT]
PflMI         129  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         161  GGGACCC              [GA]G^G[AT]CC[CT]
RsaI          177  GTAC                 GT^AC
SacII          60  CCGCGG               CCGC^GG
SexAI         695  ACCAGGT              A^CC[AT]GGT
StyI          542  CCTTGG               C^C[AT][AT]GG
TaqI          611  TCGA                 T^CGA
TfiI          230  GAATC                G^A[AT]TC
TthHB8I       611  TCGA                 T^CGA
Van91I        129  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

ApaI          394  GGGCCC               GGGCC^C
              537  GGGCCC              
AvaII         138  GGACC                G^G[AT]CC
              162  GGACC               
BanII         394  GGGCCC               G[GA]GC[CT]^C
              537  GGGCCC              
BbvCI         458  CCTCAGC              CCTCAGC(-5/-2)
              632  CCTCAGC             
BsgI           30  CTGCAC               GTGCAG(16/14)
              889  GTGCAG              
BsrBI         592  GAGCGG               CCGCTC(-3/-3)
              832  GAGCGG              
BsrI          534  ACTGG                ACTGG(1/-1)
              622  CCAGT               
BstF5I        170  CATCC                GGATG(2/0)
              675  CATCC               
DsaI           60  CCGCGG               C^C[GA][CT]GG
              616  CCACGG              
FokI          170  CATCC                GGATG(9/13)
              675  CATCC               
HgaI           51  GACGC                GACGC(5/10)
                6  GCGTC               
HphI          434  TCACC                GGTGA(8/7)
              586  TCACC               
PleI          873  GACTC                GAGTC(4/5)
              280  GAGTC               
StuI          455  AGGCCT               AGG^CCT
              776  AGGCCT              
Tsp45I          8  GTCAC                ^GT[GC]AC
              433  GTCAC               
TspRI          10  CACTG                CA[GC]TG[ACGT][ACGT]^
              234  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MaeIII      MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NgoMIV      
NlaIII      NlaIV       PstI        PvuII       Sau96I      
ScrFI       SfaNI       SfcI        TseI        
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