Restriction map for pF1KE0517
 (Linear) (Six-base) MAPSORT of: KIEE0517.seq  Check: 9554  from: 1  to: 927



KIEE0517.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     432     445     474     927 
   Size:        432      13      29     453

  Fragments arranged by size: 

                 453     432      29      13
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     898     927 
   Size:        365     533      29

  Fragments arranged by size: 

                 533     365      29
 
AfeI AGC'GCT

Cuts at:      0     376     927 
   Size:        376     551
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     914     927 
   Size:        914      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135     156     466     801     876     927 
   Size:        135      21     310     335      75      51

  Fragments arranged by size: 

                 335     310     135      75      51      21
 
ApaI G_GGCC'C

Cuts at:      0     398     541     927 
   Size:        398     143     386

  Fragments arranged by size: 

                 398     386     143
 
AvaI C'yCGr_G

Cuts at:      0      99     927 
   Size:         99     828
 
BanI G'GyrC_C

Cuts at:      0     547     927 
   Size:        547     380
 
BanII G_rGCy'C

Cuts at:      0     398     541     927 
   Size:        398     143     386

  Fragments arranged by size: 

                 398     386     143
 
BbvCI CC'TCA_GC

Cuts at:      0     459     633     927 
   Size:        459     174     294

  Fragments arranged by size: 

                 459     294     174
 
BlpI GC'TnA_GC

Cuts at:      0     910     927 
   Size:        910      17
 
Bme1580I G_kGCm'C

Cuts at:      0     398     541     552     840     855     927 
   Size:        398     143      11     288      15      72

  Fragments arranged by size: 

                 398     288     143      72      15      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     544     927 
   Size:        544     383
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     303     927 
   Size:        303     624
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     927 
   Size:        520     407
 
Bpu10I CC'TnA_GC

Cuts at:      0     459     511     633     927 
   Size:        459      52     122     294

  Fragments arranged by size: 

                 459     294     122      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205     927 
   Size:        175      30     722

  Fragments arranged by size: 

                 722     175      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220     927 
   Size:        220     707
 
BseYI C'CCAG_C

Cuts at:      0     142     486     490     578     761     927 
   Size:        142     344       4      88     183     166

  Fragments arranged by size: 

                 344     183     166     142      88       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     910     927 
   Size:         15     895      17

  Fragments arranged by size: 

                 895      17      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      43     927 
   Size:         43     884
 
Bsp1286I G_dGCh'C

Cuts at:      0     398     541     552     840     855     927 
   Size:        398     143      11     288      15      72

  Fragments arranged by size: 

                 398     288     143      72      15      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     432     445     474     927 
   Size:        432      13      29     453

  Fragments arranged by size: 

                 453     432      29      13
 
BsrBI CCG'CTC

Cuts at:      0     594     834     927 
   Size:        594     240      93

  Fragments arranged by size: 

                 594     240      93
 
BsrFI r'CCGG_y

Cuts at:      0      22     353     477     927 
   Size:         22     331     124     450

  Fragments arranged by size: 

                 450     331     124      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     801     927 
   Size:        801     126
 
BtgI C'CryG_G

Cuts at:      0      60     616     927 
   Size:         60     556     311

  Fragments arranged by size: 

                 556     311      60
 
BtsI GCAGTG_nn'

Cuts at:      0       9     927 
   Size:          9     918
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     258     293     927 
   Size:        258      35     634

  Fragments arranged by size: 

                 634     258      35
 
EaeI y'GGCC_r

Cuts at:      0     119     147     619     927 
   Size:        119      28     472     308

  Fragments arranged by size: 

                 472     308     119      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     446     927 
   Size:        446     481
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       6     927 
   Size:          6     921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     520     898     927 
   Size:        365     155     378      29

  Fragments arranged by size: 

                 378     365     155      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     754     927 
   Size:        754     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     162     394     538     927 
   Size:        153       9     232     144     389

  Fragments arranged by size: 

                 389     232     153     144       9
 
EcoRV GAT'ATC

Cuts at:      0     241     927 
   Size:        241     686
 
HaeII r_GCGC'y

Cuts at:      0     378     578     710     768     927 
   Size:        378     200     132      58     159

  Fragments arranged by size: 

                 378     200     159     132      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     659     691     927 
   Size:        154      32     473      32     236

  Fragments arranged by size: 

                 473     236     154      32      32
 
MscI TGG'CCA

Cuts at:      0     121     927 
   Size:        121     806
 
MspA1I CmG'CkG

Cuts at:      0      62     132     146     490     771     798     909     927 
   Size:         62      70      14     344     281      27     111      18

  Fragments arranged by size: 

                 344     281     111      70      62      27      18      14
            
 
NaeI GCC'GGC

Cuts at:      0      24     355     479     927 
   Size:         24     331     124     448

  Fragments arranged by size: 

                 448     331     124      24
 
NgoMIV G'CCGG_C

Cuts at:      0      22     353     477     927 
   Size:         22     331     124     450

  Fragments arranged by size: 

                 450     331     124      22
 
NspI r_CATG'y

Cuts at:      0     889     927 
   Size:        889      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     135     927 
   Size:        135     792
 
PpuMI rG'GwC_Cy

Cuts at:      0     162     927 
   Size:        162     765
 
PspOMI G'GGCC_C

Cuts at:      0     394     537     927 
   Size:        394     143     390

  Fragments arranged by size: 

                 394     390     143
 
PstI C_TGCA'G

Cuts at:      0     574     776     827     927 
   Size:        574     202      51     100

  Fragments arranged by size: 

                 574     202     100      51
 
PvuII CAG'CTG

Cuts at:      0     490     771     909     927 
   Size:        490     281     138      18

  Fragments arranged by size: 

                 490     281     138      18
 
SacII CC_GC'GG

Cuts at:      0      63     927 
   Size:         63     864
 
SanDI GG'GwC_CC

Cuts at:      0     162     927 
   Size:        162     765
 
SexAI A'CCwGG_T

Cuts at:      0     695     927 
   Size:        695     232
 
SfcI C'TryA_G

Cuts at:      0     570     772     823     927 
   Size:        570     202      51     104

  Fragments arranged by size: 

                 570     202     104      51
 
StuI AGG'CCT

Cuts at:      0     457     778     927 
   Size:        457     321     149

  Fragments arranged by size: 

                 457     321     149
 
StyI C'CwwG_G

Cuts at:      0     542     927 
   Size:        542     385
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     535     927 
   Size:        535     392
 
TatI w'GTAC_w

Cuts at:      0     176     927 
   Size:        176     751
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     426     458     927 
   Size:        426      32     469

  Fragments arranged by size: 

                 469     426      32
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII    BbvCI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BsaXI    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BstAPI     BtgI     BtsI    CspCI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     MscI   MspA1I
     NaeI   NgoMIV     NspI    PflMI    PpuMI   PspOMI     PstI    PvuII
    SacII    SanDI    SexAI     SfcI     StuI     StyI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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