Restriction map for pF1KB7683
 (Linear) (Six-base) MAPSORT of: KIBB7683.seq  Check: 4190  from: 1  to: 1038



KIBB7683.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AatII G_ACGT'C

Cuts at:      0     233    1038 
   Size:        233     805
 
AccI GT'mk_AC

Cuts at:      0     917    1038 
   Size:        917     121
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827    1038 
   Size:        827     211
 
AfeI AGC'GCT

Cuts at:      0     817    1038 
   Size:        817     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     304    1038 
   Size:        304     734
 
ApaI G_GGCC'C

Cuts at:      0     190    1038 
   Size:        190     848
 
ApaLI G'TGCA_C

Cuts at:      0     682    1038 
   Size:        682     356
 
ApoI r'AATT_y

Cuts at:      0     105    1038 
   Size:        105     933
 
BanII G_rGCy'C

Cuts at:      0     190     791    1038 
   Size:        190     601     247

  Fragments arranged by size: 

                 601     247     190
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     944    1038 
   Size:        944      94
 
BglII A'GATC_T

Cuts at:      0     211    1038 
   Size:        211     827
 
Bme1580I G_kGCm'C

Cuts at:      0     190     686    1038 
   Size:        190     496     352

  Fragments arranged by size: 

                 496     352     190
 
BmgBI CAC'GTC

Cuts at:      0     672    1038 
   Size:        672     366
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     991    1038 
   Size:        991      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     961    1038 
   Size:        141     820      77

  Fragments arranged by size: 

                 820     141      77
 
BsaHI Gr'CG_yC

Cuts at:      0     230    1038 
   Size:        230     808
 
BsaWI w'CCGG_w

Cuts at:      0      10    1038 
   Size:         10    1028
 
BsiHKAI G_wGCw'C

Cuts at:      0     686    1038 
   Size:        686     352
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      37    1038 
   Size:         37    1001
 
Bsp1286I G_dGCh'C

Cuts at:      0     190     686     791    1038 
   Size:        190     496     105     247

  Fragments arranged by size: 

                 496     247     190     105
 
BspEI T'CCGG_A

Cuts at:      0      10    1038 
   Size:         10    1028
 
BsrDI GCAATG_nn'

Cuts at:      0     618    1038 
   Size:        618     420
 
BsrGI T'GTAC_A

Cuts at:      0     405    1038 
   Size:        405     633
 
BstYI r'GATC_y

Cuts at:      0     211    1038 
   Size:        211     827
 
BstZ17I GTA'TAC

Cuts at:      0     918    1038 
   Size:        918     120
 
EarI CTCTTCn'nnn_

Cuts at:      0     872    1004    1038 
   Size:        872     132      34

  Fragments arranged by size: 

                 872     132      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827    1038 
   Size:        827     211
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     194    1038 
   Size:        194     844
 
EcoO109I rG'GnC_Cy

Cuts at:      0     187    1038 
   Size:        187     851
 
HaeII r_GCGC'y

Cuts at:      0     819    1038 
   Size:        819     219
 
HindIII A'AGCT_T

Cuts at:      0     644    1038 
   Size:        644     394
 
MslI CAynn'nnrTG

Cuts at:      0     329    1038 
   Size:        329     709
 
PasI CC'CwG_GG

Cuts at:      0     190     383    1038 
   Size:        190     193     655

  Fragments arranged by size: 

                 655     193     190
 
PfoI T'CCnGG_A

Cuts at:      0     113     273     636    1038 
   Size:        113     160     363     402

  Fragments arranged by size: 

                 402     363     160     113
 
PspOMI G'GGCC_C

Cuts at:      0     186    1038 
   Size:        186     852
 
SfcI C'TryA_G

Cuts at:      0     477    1038 
   Size:        477     561
 
SmlI C'TyrA_G

Cuts at:      0     120     976    1038 
   Size:        120     856      62

  Fragments arranged by size: 

                 856     120      62
 
SspI AAT'ATT

Cuts at:      0     471    1038 
   Size:        471     567
 
TatI w'GTAC_w

Cuts at:      0     405    1038 
   Size:        405     633
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      92     124    1038 
   Size:         92      32     914

  Fragments arranged by size: 

                 914      92      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     663    1038 
   Size:        663     375
 
ZraI GAC'GTC

Cuts at:      0     231    1038 
   Size:        231     807
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI    AlwNI     ApaI    ApaLI     ApoI
    BanII     BbsI    BglII Bme1580I    BmgBI     BplI    BpuEI    BsaHI
    BsaWI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrDI    BsrGI    BstYI
  BstZ17I     EarI  Eco57MI    EcoNI EcoO109I    HaeII  HindIII     MslI
     PasI     PfoI   PspOMI     SfcI     SmlI     SspI     TatI     TstI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     XmnI
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