Restriction map for pF1KE0195
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# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        ApoI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        TfiI        Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI         1187  GTAGAC               GT^[AC][GT]AC
AfaI         1194  GTAC                 GT^AC
Alw44I       1083  GTGCAC               G^TGCAC
AlwNI         361  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1083  GTGCAC               G^TGCAC
AscI         1139  GGCGCGCC             GG^CGCGCC
BciVI         238  GTATCC               GTATCC(6/5)
BclI         1067  TGATCA               T^GATCA
BfaI          127  CTAG                 C^TAG
BglI           92  GCCCCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1101  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         727  GAGGAG               GAGGAG(10/8)
BsgI         1089  CTGCAC               GTGCAG(16/14)
DraI            6  TTTAAA               TTT^AAA
DrdI          484  GACGGGCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          829  GAAGAG               CTCTTC(1/4)
FauI          972  CCCGC                CCCGC(4/6)
FbaI         1067  TGATCA               T^GATCA
HincII        493  GTCAAC               GT[CT]^[GA]AC
HindII        493  GTCAAC               GT[CT]^[GA]AC
Ksp632I       829  GAAGAG               CTCTTC(1/4)
MaeI          127  CTAG                 C^TAG
MaeII         559  ACGT                 A^CGT
MamI         1101  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI            7  TTAA                 T^TAA
RsaI         1194  GTAC                 GT^AC
SbfI          774  CCTGCAGG             CCTGCA^GG
SexAI         257  ACCAGGT              A^CC[AT]GGT
SfiI           91  GGCCCCAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          229  CTCAAG               C^T[CT][GA]AG
Sse8387I      774  CCTGCAGG             CCTGCA^GG
TaqI          326  TCGA                 T^CGA
Tru9I           7  TTAA                 T^TAA
TthHB8I       326  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaI          385  CCCGAG               C^[CT]CG[GA]G
              466  CCCGAG              
BanII          27  GAGCCC               G[GA]GC[CT]^C
              460  GAGCCC              
BbsI           85  GAAGAC               GAAGAC(2/6)
              481  GAAGAC              
BpuAI          85  GAAGAC               GAAGAC(2/6)
              481  GAAGAC              
BsaI          886  GAGACC               GGTCTC(1/5)
              686  GGTCTC              
BsoBI         385  CCCGAG               C^[CT]CG[GA]G
              466  CCCGAG              
BssHII        300  GCGCGC               G^CGCGC
             1140  GCGCGC              
BstAPI        255  GCACCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              702  GCAGCTGTTGC         
EcoNI         720  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1127  CCTGGCAGAGG         
EcoT14I       863  CCAAGG               C^C[AT][AT]GG
             1199  CCAAGG              
MslI         1076  CACGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1095  CATGCTGATG          
PpuMI        1118  GGGTCCT              [GA]G^G[AT]CC[CT]
             1172  GGGTCCC             
StuI          123  AGGCCT               AGG^CCT
              278  AGGCCT              
StyI          863  CCAAGG               C^C[AT][AT]GG
             1199  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaII       
BanI        BbvI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      TspRI       Van91I      
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