Restriction map for pF1KE0195
 (Linear) (Six-base) MAPSORT of: KIEE0195.seq  Check: 4562  from: 1  to: 1230



KIEE0195.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1096    1230 
   Size:       1096     134
 
AccI GT'mk_AC

Cuts at:      0    1188    1230 
   Size:       1188      42
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1230 
   Size:        706     524
 
AleI CACnn'nnGTG

Cuts at:      0    1080    1230 
   Size:       1080     150
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366    1230 
   Size:        366     864
 
ApaLI G'TGCA_C

Cuts at:      0    1083    1230 
   Size:       1083     147
 
AscI GG'CGCG_CC

Cuts at:      0    1140    1230 
   Size:       1140      90
 
AvaI C'yCGr_G

Cuts at:      0     385     466    1230 
   Size:        385      81     764

  Fragments arranged by size: 

                 764     385      81
 
BanI G'GyrC_C

Cuts at:      0     155     254     261    1230 
   Size:        155      99       7     969

  Fragments arranged by size: 

                 969     155      99       7
 
BanII G_rGCy'C

Cuts at:      0      31     464    1230 
   Size:         31     433     766

  Fragments arranged by size: 

                 766     433      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     488    1230 
   Size:         92     396     742

  Fragments arranged by size: 

                 742     396      92
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     249    1230 
   Size:        249     981
 
BclI T'GATC_A

Cuts at:      0    1067    1230 
   Size:       1067     163
 
BglI GCCn_nnn'nGGC

Cuts at:      0      98    1230 
   Size:         98    1132
 
Bme1580I G_kGCm'C

Cuts at:      0    1087    1230 
   Size:       1087     143
 
BmgBI CAC'GTC

Cuts at:      0     560    1230 
   Size:        560     670
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      89     431    1230 
   Size:         89     342     799

  Fragments arranged by size: 

                 799     342      89
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     388     640     733     934     961    1164    1199 
   Size:        343      45     252      93     201      27     203      35

Cuts at:   1199    1230 
   Size:         31

  Fragments arranged by size: 

                 343     252     203     201      93      45      35      31
                  27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214    1230 
   Size:        214    1016
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     692     880    1230 
   Size:        692     188     350

  Fragments arranged by size: 

                 692     350     188
 
BsaBI GATnn'nnATC

Cuts at:      0    1105    1230 
   Size:       1105     125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     718     748    1230 
   Size:        718      30     482

  Fragments arranged by size: 

                 718     482      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     742    1230 
   Size:        742     488
 
BseYI C'CCAG_C

Cuts at:      0      31     417     584    1230 
   Size:         31     386     167     646

  Fragments arranged by size: 

                 646     386     167      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1074    1230 
   Size:       1074     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     956    1087    1230 
   Size:        332     624     131     143

  Fragments arranged by size: 

                 624     332     143     131
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     332     464     956    1087    1230 
   Size:         31     301     132     492     131     143

  Fragments arranged by size: 

                 492     301     143     132     131      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     506     605     707    1096    1230 
   Size:        506      99     102     389     134

  Fragments arranged by size: 

                 506     389     134     102      99
 
BsrFI r'CCGG_y

Cuts at:      0      65     251     489    1230 
   Size:         65     186     238     741

  Fragments arranged by size: 

                 741     238     186      65
 
BssHII G'CGCG_C

Cuts at:      0     300    1140    1230 
   Size:        300     840      90

  Fragments arranged by size: 

                 840     300      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     261     708    1230 
   Size:        261     447     522

  Fragments arranged by size: 

                 522     447     261
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     300    1230 
   Size:        300     930
 
DraI TTT'AAA

Cuts at:      0       8    1230 
   Size:          8    1222
 
DraIII CAC_nnn'GTG

Cuts at:      0     261     439    1046    1230 
   Size:        261     178     607     184

  Fragments arranged by size: 

                 607     261     184     178
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     490    1230 
   Size:        490     740
 
EarI CTCTTCn'nnn_

Cuts at:      0     824    1230 
   Size:        824     406
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28    1230 
   Size:         28    1202
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     388     640     706     733     934     961    1164 
   Size:        343      45     252      66      27     201      27     203

Cuts at:   1164    1199    1230 
   Size:         35      31

  Fragments arranged by size: 

                 343     252     203     201      66      45      35      31
                  27      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     724    1131    1230 
   Size:        724     407      99

  Fragments arranged by size: 

                 724     407      99
 
EcoO109I rG'GnC_Cy

Cuts at:      0     447     515    1119    1173    1230 
   Size:        447      68     604      54      57

  Fragments arranged by size: 

                 604     447      68      57      54
 
HincII GTy'rAC

Cuts at:      0     495    1230 
   Size:        495     735
 
MslI CAynn'nnrTG

Cuts at:      0    1080    1099    1230 
   Size:       1080      19     131

  Fragments arranged by size: 

                1080     131      19
 
MspA1I CmG'CkG

Cuts at:      0     183     705     786     949    1230 
   Size:        183     522      81     163     281

  Fragments arranged by size: 

                 522     281     183     163      81
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     366    1160    1222    1230 
   Size:        366     794      62       8

  Fragments arranged by size: 

                 794     366      62       8
 
PfoI T'CCnGG_A

Cuts at:      0     359     656    1230 
   Size:        359     297     574

  Fragments arranged by size: 

                 574     359     297
 
PpuMI rG'GwC_Cy

Cuts at:      0    1119    1173    1230 
   Size:       1119      54      57

  Fragments arranged by size: 

                1119      57      54
 
PstI C_TGCA'G

Cuts at:      0     382     704     779     826    1230 
   Size:        382     322      75      47     404

  Fragments arranged by size: 

                 404     382     322      75      47
 
PvuII CAG'CTG

Cuts at:      0     183     705     786    1230 
   Size:        183     522      81     444

  Fragments arranged by size: 

                 522     444     183      81
 
SanDI GG'GwC_CC

Cuts at:      0    1173    1230 
   Size:       1173      57
 
SbfI CC_TGCA'GG

Cuts at:      0     779    1230 
   Size:        779     451
 
SexAI A'CCwGG_T

Cuts at:      0     257    1230 
   Size:        257     973
 
SfcI C'TryA_G

Cuts at:      0     378     700     775     822     922    1230 
   Size:        378     322      75      47     100     308

  Fragments arranged by size: 

                 378     322     308     100      75      47
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      98    1230 
   Size:         98    1132
 
SmlI C'TyrA_G

Cuts at:      0     229    1230 
   Size:        229    1001
 
StuI AGG'CCT

Cuts at:      0     125     280    1230 
   Size:        125     155     950

  Fragments arranged by size: 

                 950     155     125
 
StyI C'CwwG_G

Cuts at:      0     863    1199    1230 
   Size:        863     336      31

  Fragments arranged by size: 

                 863     336      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     450     949    1230 
   Size:        450     499     281

  Fragments arranged by size: 

                 499     450     281
 
TatI w'GTAC_w

Cuts at:      0    1193    1230 
   Size:       1193      37
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     718     750    1230 
   Size:        718      32     480

  Fragments arranged by size: 

                 718     480      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI    ApaLI     AscI     AvaI
     BanI    BanII     BbsI    BciVI     BclI     BglI Bme1580I    BmgBI
     BmrI     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII   BstAPI    BtgZI
     DraI   DraIII     DrdI     EarI     EciI  Eco57MI    EcoNI EcoO109I
   HincII     MslI   MspA1I    PflMI     PfoI    PpuMI     PstI    PvuII
    SanDI     SbfI    SexAI     SfcI     SfiI     SmlI     StuI     StyI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BfrBI    BglII     BlpI     BmtI
     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     EaeI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     ScaI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com